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General Information
Symbol
Dmel\HDAC6KO
Species
D. melanogaster
Name
FlyBase ID
FBal0248834
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dHDAC6KO
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:
Mutation in ATG codon and frameshift introduced by homologous recombination. AAC[ATG]GT (start codon indicated) replaced with AGATCT.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Mutation in the translation start codon and a frame shift in the HDAC6 open reading frame.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
HDAC6KO mutant larvae show a significantly increased response to gentle touch, as compared to controls.
HDAC6KO mutant third instar larvae display mild but significant increase in the density of perinuclear microtubule network in muscle cells compared to controls at room temperature and their microtubules (MT) are also more resistant to cold-induced destabilization (the perinuclear MTs are significantly denser in HDAC6KO mutant muscle cells than in wild-type after cold treatment).
HDAC6KO mutants do not exhibit macro-morphological changes in synaptic features, with the number of T-bars, synaptic vesicles, or mitochondria per boutonic area, not differing from controls. However, using serial-section election microscopy, HDAC6KO mutant T-bars exhibit significantly smaller 'top sizes' and fewer tethered synaptic vesicles compared to controls. At low-frequency stimulation, the excitatory junctional current amplitude in HDAC6KO mutants is marginally smaller.
Homozygotes show no obvious morphological abnormalities under normal culture conditions. Mutants do not show any notable difference to wild-type animals in response to oxidative or heat stress. Mutant adults do not show defects in climbing ability (assayed up to 20 days after eclosion).
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
HDAC6KO is a non-suppressor of microtubule phenotype of Scer\GAL4C57, spasUAS.cTa
Additional Comments
Genetic Interactions
Statement
Reference
The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing spasScer\UAS.cTa under the control of Scer\GAL4C57 is not altered by the presence of HDAC6KO. The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing Kat60Scer\UAS.cMa under the control of Scer\GAL4C57 is not altered by the presence of HDAC6KO. The microtubule fibres are significantly shorter in the double mutant cells.
Xenogenetic Interactions
Statement
Reference
A HDAC6KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBPA315T.cMa.Scer\UAS under the control of Scer\GAL4elav.PU. A HDAC6KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBPA382T.cMa.Scer\UAS under the control of Scer\GAL4elav.PU. While heterozygosity for HDAC6KO alone has no effect on activity or negative geotaxis, these conditions both significant suppress the activity and geotaxis defects in flies expressing Hsap\TARDBPA315T.cMa.Scer\UAS under the control of Scer\GAL4elav.PU.
HDAC6KO and HDAC6KO/Df(1)ED7294 each rescue the defects in perinuclear microtubule density seen in the muscles of animals expressing either Hsap\MAPTScer\UAS.cWa or Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4C57. HDAC6KO completely suppresses the increase in the number of satellite boutons at the neuromuscular junction (NMJ) and the decrease in average NMJ length caused by expression of either Hsap\MAPTScer\UAS.cWa or Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4elav.PU.
HDAC6KO enhances the loss of dopaminergic (DA) neurons caused by expression of Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4ple.PF; the neuron degeneration phenotype is apparent at 10 days after eclosion in the double mutant flies, and 20 days after eclosion it is more severe than that seen in the Hsap\SNCAScer\UAS.cFa, Scer\GAL4ple.PF single mutants. HDAC6KO enhances the retinal degeneration phenotype seen in adults expressing Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4GMR.PU. HDAC6KO enhances the climbing ability defect caused by expression of Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4ple.PF; defects are seen 10 days after eclosion in the double mutant flies (defects are not seen until 20 days after eclosion in Hsap\SNCAScer\UAS.cFa, Scer\GAL4ple.PF single mutants) and the defects at 20 days after eclosion are more severe in the double mutants. HDAC6KO decreases the number of Lewy body-like Hsap\SNCA positive inclusions seen in the brains of adults expressing Hsap\SNCAScer\UAS.cFa under the control of Scer\GAL4ple.PF such that no inclusions are seen in 10 day old flies and the number of inclusions seen in 20 days old flies is reduced.
Complementation and Rescue Data
Partially rescued by
Comments
Expression of either HDAC6Scer\UAS.cDa or HDAC6H237A.Scer\UAS under the control of Scer\GAL4C57 completely suppresses the ability of HDAC6KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4C57. Expression of HDAC6H664A.Scer\UAS under the control of Scer\GAL4C57 partially suppresses the ability of HDAC6KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4C57. Expression of HDAC6H237A.H664A.Scer\UAS under the control of Scer\GAL4C57 does not affect the ability of HDAC6KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4C57.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)