mutant larvae show a significantly increased response to gentle touch, as compared to controls.
mutant third instar larvae display mild but significant increase in the density of perinuclear microtubule network in muscle cells compared to controls at room temperature and their microtubules (MT) are also more resistant to cold-induced destabilization (the perinuclear MTs are significantly denser in HDAC6KO
mutant muscle cells than in wild-type after cold treatment).
mutants do not exhibit macro-morphological changes in synaptic features, with the number of T-bars, synaptic vesicles, or mitochondria per boutonic area, not differing from controls. However, using serial-section election microscopy, HDAC6KO
mutant T-bars exhibit significantly smaller 'top sizes' and fewer tethered synaptic vesicles compared to controls.
At low-frequency stimulation, the excitatory junctional current amplitude in HDAC6KO
mutants is marginally smaller.
Homozygotes show no obvious morphological abnormalities under normal culture conditions.
Mutants do not show any notable difference to wild-type animals in response to oxidative or heat stress.
Mutant adults do not show defects in climbing ability (assayed up to 20 days after eclosion).