Allele Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG}	Species	H. sapiens
Name		FlyBase ID	FBal0248853
Feature type	allele	Associated gene	Hsap\TARDBP

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Miguel et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\TARDBP coding sequences. (Miguel et al., 2011)
Carried in construct	P{UAS-Hsap\TARDBP-FLAG} (Miguel et al., 2011)
Tagged with	T:Zzzz\FLAG (Miguel et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^{elav.Switch.PO} (Miguel et al., 2011) lethal \| first instar larval stage, with Scer\GAL4^elav-C155 (Miguel et al., 2011) lethal \| larval stage, with Scer\GAL4^how-24B (Miguel et al., 2011) lethal \| pupal stage, with Scer\GAL4^repo (Miguel et al., 2011) short lived \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Miguel et al., 2011)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Miguel et al., 2011) eye \| heat sensitive, with Scer\GAL4^GMR.PF (Miguel et al., 2011) interommatidial bristle, with Scer\GAL4^GMR.PF (Miguel et al., 2011) interommatidial bristle \| heat sensitive, with Scer\GAL4^GMR.PF (Miguel et al., 2011) ommatidium, with Scer\GAL4^GMR.PF (Miguel et al., 2011) ommatidium \| heat sensitive, with Scer\GAL4^GMR.PF (Miguel et al., 2011)
Detailed Description
	Statement Reference Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] neuronally under the control of Scer\GAL4[elav.Switch.PO] at 25[o]C results in early death. Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] neuronally under the control of Scer\GAL4[elav-C155] at 25[o]C results in L1 larval lethality. Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] in glia in the developing nervous system under the control of Scer\GAL4[repo] at 25[o]C results in pupal lethality. Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] in developing muscle cells under the control of Scer\GAL4[how-24B] at 25[o]C results in larval lethality. Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] in the developing eye under the control of Scer\GAL4[GMR.PF] at 25[o]C results in a mild rough eye phenotype with minor disruption in the external lattice with occasionally interommatidial bristles missing. Expression at 29[o]C results in an additional loss of pigment phenotype. These phenotypes worsen over time. Expression of Hsap\TARDBP[M.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav.Switch.PO] (in a RU486 background) greatly reduces adult fly longevity. Examination of the brains of 20 day old mutants fails to identify any obvious abnormalities such as vacuolar pathology. (Miguel et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Miguel et al., 2011, Neurobiol. Disease 41(2): 398--406 Both cytoplasmic and nuclear accumulations of the protein are neurotoxic in Drosophila models of TDP-43 proteinopathies. [FBrf0212655]

Allele Hsap\TARDBPM.Scer\UAS.T:Zzzz\FLAG

Allele Hsap\TARDBP^{M.Scer\UAS.T:Zzzz\FLAG}