Allele Hsap\TARDBP^{mutNES.Scer\UAS}

General Information
Symbol	Hsap\TARDBP^{mutNES.Scer\UAS}	Species	H. sapiens
Name		FlyBase ID	FBal0248855
Feature type	allele	Associated gene	Hsap\TARDBP

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - site directed mutagenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Miguel et al., 2011) in vitro construct - site directed mutagenesis (Miguel et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\TARDBP sequences where the nuclear export signal (NES) is mutated. (Miguel et al., 2011)
Carried in construct	P{UAS-Hsap\TARDBPmutNES-FLAG} (Miguel et al., 2011)
Tagged with	T:Zzzz\FLAG (Miguel et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^{elav.Switch.PO} (Miguel et al., 2011) lethal \| pupal stage, with Scer\GAL4^elav-C155 (Miguel et al., 2011) short lived \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Miguel et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Hsap\TARDBP[mutNES.Scer\UAS] neuronally under the control of Scer\GAL4[elav.Switch.PO] at 25[o]C results in early death. Expression of Hsap\TARDBP[mutNES.Scer\UAS] neuronally under the control of Scer\GAL4[elav-C155] at 25[o]C results in pupal lethality (with a few rare escapers). Glial expression of Hsap\TARDBP[mutNES.Scer\UAS] in the developing nervous system under the control of Scer\GAL4[repo] at 25[o]C does not generate a phenotype. Expression of Hsap\TARDBP[mutNES.Scer\UAS] in developing muscle cells under the control of Scer\GAL4[how-24B] at 25[o]C does not generate a phenotype. Expression of Hsap\TARDBP[mutNES.Scer\UAS] in the developing eye under the control of Scer\GAL4[GMR.PF] at 25[o]C does not generate a phenotype. Expression of Hsap\TARDBP[mutNES.Scer\UAS] in the developing eye under the control of Scer\GAL4[GMR.PF] at 29[o]C does not generate a phenotype. Expression of Hsap\TARDBP[mutNES.Scer\UAS] under the control of Scer\GAL4[elav.Switch.PO] (in a RU486 background) greatly reduces adult fly longevity (though not as much as expression of the wild-type form). (Miguel et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhancer of
Statement Reference Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, TER94^Scer\UAS.cRa (Ritson et al., 2010)
NOT Suppressor of
Statement Reference Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, TER94^Scer\UAS.cRa (Ritson et al., 2010)
Phenotype Manifest In
NOT Enhancer of
Statement Reference Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, TER94^Scer\UAS.cRa (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010)
NOT Suppressor of
Statement Reference Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, TER94^Scer\UAS.cRa (Ritson et al., 2010) Hsap\TARDBP^{mutNES.Scer\UAS}, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, TER94^{R152H.Scer\UAS.R} (Ritson et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\TARDBP^{mutNES.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Miguel et al., 2011, Neurobiol. Disease 41(2): 398--406 Both cytoplasmic and nuclear accumulations of the protein are neurotoxic in Drosophila models of TDP-43 proteinopathies. [FBrf0212655] Ritson et al., 2010, J. Neurosci. 30(22): 7729--7739 TDP-43 mediates degeneration in a novel Drosophila model of disease caused by mutations in VCP/p97. [FBrf0210957]

Allele Hsap\TARDBPmutNES.Scer\UAS

Allele Hsap\TARDBP^{mutNES.Scer\UAS}