FlyBase Allele Report: Dmel\stet[1A3]

General Information

Symbol

Dmel\stet^1A3

Species

D. melanogaster

Name

FlyBase ID

FBal0248860

Feature type

allele

Associated gene

Dmel\stet

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Liu et al., 2010)

Progenitor genotype

Caused by aberration

Df(3L)stet^1A3

Cytology

Description

A deletion allele resulting from the imprecise excision of a P-element. The deleted region includes that part of stet which encodes a seven transmembrane domain believed to be necessary for enzymatic activity.

(Liu et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Liu et al., 2010)

viable

(Liu et al., 2010)

Phenotype Manifest In

germarium

(Liu et al., 2010)

germarium | germline clone

(Liu et al., 2010)

spectrosome | germline clone | increased number

(Liu et al., 2010)

spectrosome | increased number

(Liu et al., 2010)

Detailed Description

Statement

Reference

stet^1A3 homozygotes are viable but sterile.

Germaria from stet^1A3 mutant animals exhibit supernumerary spectrosome-containing cells. Virtually identical phenotypes are observed when stet function is specifically removed from the germ line. Germaria with a single stet^1A3 mutant germline stem cell clone exhibit non-cell autonomous defects regarding supernumerary spectrosome-containing cells.

stet^1A3 mutant germaria have similar numbers of cap cells as wild-type ones.

(Liu et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

stet^1A3 has germarium phenotype, suppressible by dally^GD5918/Scer\GAL4^unspecified

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, suppressible by dally^GD5918/Scer\GAL4^unspecified

(Liu et al., 2010)

stet^1A3 has germarium phenotype, suppressible by Krn^{s.UASp.Tag:MYC}/Scer\GAL4^nanos.PU

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, suppressible by Krn^{s.UASp.Tag:MYC}/Scer\GAL4^nanos.PU

(Liu et al., 2010)

stet^1A3 has germarium phenotype, suppressible | partially by Scer\GAL4^C587/Raf^UAS.F179

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, suppressible | partially by Scer\GAL4^C587/Raf^UAS.F179

(Liu et al., 2010)

stet^1A3 has germarium phenotype, suppressible by tkv[+]/tkv^P906

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, suppressible by tkv[+]/tkv^P906

(Liu et al., 2010)

stet^1A3 has germarium phenotype, suppressible by dpp^hr56/dpp[+]

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, suppressible by dpp^hr56/dpp[+]

(Liu et al., 2010)

NOT suppressed by

Statement

Reference

stet^1A3 has germarium phenotype, non-suppressible by dlp^VDRC.cUa/Scer\GAL4^unspecified

(Liu et al., 2010)

stet^1A3 has spectrosome | increased number phenotype, non-suppressible by dlp^VDRC.cUa/Scer\GAL4^unspecified

(Liu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The supernumerary spectrosome-containing cell phenotype of stet^1A3 mutants is suppressed by Krn^{s.Scer\UAS.T:Hsap\MYC} expression driven by Scer\GAL4^nos.PU.

The supernumerary spectrosome-containing cell phenotype of stet^1A3 mutants is partially suppressed by phl^{Scer\UAS.F179} expression driven by Scer\GAL4^C587.

tkv^P906/+ suppresses the formation of ectopic spectrosome-containing cells in stet^1A3 mutant germaria.

dpp^hr56/+ suppresses the formation of ectopic spectrosome-containing cells in stet^1A3 mutant germaria.

Expression of dlp^VDRC.cUa driven by Scer\GAL4^unspecified in escort stem cells and escort cells fails to suppresses the supernumerary spectrosome-containing cell phenotype in stet^1A3 mutant germaria.

Expression of dally^GD5918 driven by Scer\GAL4^unspecified in escort stem cells and escort cells partially suppresses the supernumerary spectrosome-containing cell phenotype in stet^1A3 mutant germaria.

(Liu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

stet^1A3 is rescued by stet^UASp.cGa/Scer\GAL4^nanos.PU

(Liu et al., 2010)

Partially rescued by

stet^1A3 is partially rescued by stet^UASp.cGa/Scer\GAL4^VP16.bam

(Liu et al., 2010)

stet^1A3 is partially rescued by stet^bam.PL

(Liu et al., 2010)

Comments

The female sterility and the supernumerary spectrosome-containing cell phenotype of stet^1A3 mutants is rescued by the expression of stet^{Scer\UAS.P\T.cGa} driven by Scer\GAL4^nos.PU in the germ line.

stet^bam.PL largely reverts the supernumerary spectrosome-containing cell phenotype and rescues the female sterility of stet^1A3 mutants. However, many stet^1A3 mutant germaria still have more spectrosome-containing cells than their wild-type counterparts.

The supernumerary spectrosome-containing cell phenotype of stet^1A3 mutants is partially reverted by the expression of stet^{Scer\UAS.P\T.cGa} driven by Scer\GAL4^{bam.T:Hsim\VP16}.

(Liu et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

stet^1A3

(Zhao et al., 2018, Liu et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (2)

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