FlyBase Allele Report: Dmel\Su(var)3-3[ΔN]

General Information

Symbol

Dmel\Su(var)3-3^ΔN

Species

D. melanogaster

Name

FlyBase ID

FBal0248941

Feature type

allele

Associated gene

Dmel\Su(var)3-3

Associated Insertion(s)

PBac{WHr}Su(var)3-3^ΔN

Carried in Construct

Also Known As

Lsd1^ΔN, dLsd1^ΔN

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Di Stefano et al., 2007)

Progenitor genotype

PBac{WH}Su(var)3-3^f00678

(Di Stefano et al., 2007)

PBac{WH}f03544

(Di Stefano et al., 2007)

Associated Insertion(s)

PBac{WHr}Su(var)3-3^ΔN

Cytology

Description

Recombination between the two progenitor insertions has resulted in the deletion of the sequence between them, removing the presumptive promoter region and N-terminal portion of Su(var)3-3.

(Di Stefano et al., 2007)

Allele components

Component

Use(s)

Inserted element

PBac{WHr}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | oogenesis

(Shao et al., 2022)

female sterile, with Scer\GAL4^C587, Su(var)3-3^UAS.cDa

(Eliazer et al., 2011)

female sterile | recessive

(Di Stefano et al., 2007)

flightless | recessive

(Di Stefano et al., 2007)

increased cell number | cell non-autonomous | oogenesis | somatic clone

(Eliazer et al., 2014)

lethal | male | recessive

(Di Stefano et al., 2011)

male sterile | recessive

(Di Stefano et al., 2007)

partially lethal - majority die | male | recessive

(Di Stefano et al., 2007)

partially lethal - majority die | recessive

(Di Stefano et al., 2007)

suppressor of variegation, with In(1)y^3P

(Dietz et al., 2015)

suppressor of variegation, with T(2;3)Sb^V

(Dietz et al., 2015)

suppressor of variegation | dominant

(Di Stefano et al., 2011, Di Stefano et al., 2007)

visible

(Di Stefano et al., 2011)

visible | adult stage

(Dietz et al., 2015)

visible | recessive

(Di Stefano et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

The positional effect variegation (PEV) at the Sb locus caused by T(2;3)Sb^V or at the y locus caused by In(1)y^3P is significantly suppressed by single copy of Su(var)3-3^ΔN. Su(var)3-3^ΔN homozygous mutants also display ectopic vein material at the posterior crossvein, although the penetrance is rather low.

(Dietz et al., 2015)

Ovaries bearing Su(var)3-3^ΔN escort cell clones, namely middle escort clone, can show increased number of germline stem cell-like cells; in these cases, germaria can show an elongated morphology.

(Eliazer et al., 2014)

Heterozygotes have normal wings.

(Curtis et al., 2011)

36% of homozygotes have ectopic wing vein tissue, which emanates from the posterior crossvein, posterior to the L5 longitudinal vein.

80% of homozygotes have a held-out wing phenotype.

The number of prefollicle and follicle cells is strongly reduced in mutant ovaries, and they fail to completely encapsulate the germline cyst.

Su(var)3-3^ΔN/+ suppresses the variegation at the w locus which is seen in the In(1)w^m4h chromosome and suppresses the variegation at the mutant Sb locus which is seen in the T(2;3)Sb^V chromosome.

(Di Stefano et al., 2011)

Compared with wild-type controls, homozygous Su(var)3-3^ΔN mutant ovaries contain an increased number of single undifferentiated germline stem cell-like cells with round fusomes.

Homozygous Su(var)3-3^ΔN mutant germline clones differentiate into morphologically normal egg chambers without any apparent block in differentiation even over long periods of time. The Su(var)3-3^ΔN mutant germline stem cell (GSC) clones are maintained at levels similar to control clones.

Homozygous Su(var)3-3^ΔN mutant follicle cell clones appear normal and are able to envelope germline cysts fully without any obvious defects. Su(var)3-3^ΔN mutant follicle cell clones do not appear to engender an abnormal number of GSCs. Su(var)3-3^ΔN mutant follicle stem cell clones are maintained over long periods of time.

Expression of Su(var)3-3^Scer\UAS.cDa under the control of Scer\GAL4^C587 rescues the germarium and ovariole phenotypes of Su(var)3-3^ΔN mutants. However, the fully matured eggs produced by the Su(var)3-3^ΔN mutants females expressing Su(var)3-3^Scer\UAS.cDa remain sterile.

(Eliazer et al., 2011)

Crosses of heterozygotes produce only one-third of the expected number of homozygous progeny. The reduction in viability of homozygotes is more dramatic in the male progeny (approximately 90% of the recovered homozygotes are females).

Homozygous females lack proper ovariole structures in the ovary. The formation of egg chambers is abnormal at very early stages. Both germline and follicle cells appear abnormal and the follicle cells fails to encapsulate the 16-cell cysts.

The testes appear morphologically intact in homozygous males, but DNA staining suggest defects during spermatogenesis.

Homozygotes have a held-out wing phenotype and are flightless.

Su(var)3-3^ΔN/+ suppresses the variegation at the mutant Sb locus which is seen in the T(2;3)Sb^V chromosome and suppresses the variegation at the y locus which is seen in the In(1)y^3P chromosome.

(Di Stefano et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Su(var)3-3^ΔN has visible | adult stage phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Suppressed by

Statement

Reference

Su(var)3-3^ΔN has decreased cell number | oogenesis phenotype, suppressible by Df(3L)LINR-2Δ

(Shao et al., 2022)

Su(var)3-3^ΔN has visible phenotype, suppressible | partially by lid[+]/Kdm5¹⁰⁴²⁴

(Di Stefano et al., 2011)

Su(var)3-3^ΔN has lethal | recessive | male phenotype, suppressible | partially by lid[+]/Kdm5¹⁰⁴²⁴

(Di Stefano et al., 2011)

Enhancer of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of visible | dominant phenotype of mor³

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of visible | dominant phenotype of mor⁶

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of visible | dominant phenotype of polybromo^EY14080

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of visible | dominant phenotype of polybromo^EY14730

(Curtis et al., 2011)

NOT Enhancer of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a non-enhancer of visible | dominant phenotype of Snr1^E1

(Curtis et al., 2011)

Suppressor of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a suppressor of visible | dominant phenotype of mor⁵

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is a suppressor of visible | dominant phenotype of N^Ax-16

(Di Stefano et al., 2011)

NOT Suppressor of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a non-suppressor of visible | dominant phenotype of Snr1^E1

(Curtis et al., 2011)

Other

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN, brm² has visible | dominant phenotype

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN, mor¹ has visible | dominant phenotype

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN, polybromo^Δ86 has visible | dominant phenotype

(Curtis et al., 2011)

Bap170^Δ65, Su(var)3-3[+]/Su(var)3-3^ΔN has visible | dominant phenotype

(Curtis et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Su(var)3-3^ΔN has posterior crossvein phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Suppressed by

Statement

Reference

Su(var)3-3^ΔN has main body follicle cell | decreased number phenotype, suppressible by Df(3L)LINR-2Δ

(Shao et al., 2022)

Su(var)3-3^ΔN has wing vein | ectopic phenotype, suppressible by lid[+]/Kdm5¹⁰⁴²⁴

(Di Stefano et al., 2011)

Su(var)3-3^ΔN has wing phenotype, suppressible | partially by lid[+]/Kdm5¹⁰⁴²⁴

(Di Stefano et al., 2011)

Su(var)3-3^ΔN has follicle cell phenotype, suppressible | partially by lid[+]/Kdm5¹⁰⁴²⁴

(Di Stefano et al., 2011)

Su(var)3-3^ΔN has ovary phenotype, suppressible | partially by Su(H)[+]/Su(H)⁰⁵

(Di Stefano et al., 2011)

Su(var)3-3^ΔN has female germline stem cell | increased number phenotype, suppressible | partially by dpp^hr92

(Eliazer et al., 2011)

Su(var)3-3^ΔN has female germline stem cell | increased number phenotype, suppressible | partially by dpp^hr56

(Eliazer et al., 2011)

NOT suppressed by

Statement

Reference

Su(var)3-3^ΔN has ovary phenotype, non-suppressible by Scer\GAL4^Act5C.PI/piwi^{UASp.tdTomato}

(Lepesant et al., 2020)

Enhancer of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of wing vein | ectopic phenotype of mor³

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of wing vein | ectopic phenotype of mor⁶

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of wing vein | ectopic phenotype of polybromo^EY14080

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is an enhancer of wing vein | ectopic phenotype of polybromo^EY14730

(Curtis et al., 2011)

NOT Enhancer of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a non-enhancer of wing vein | ectopic phenotype of Snr1^E1

(Curtis et al., 2011)

Suppressor of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a suppressor of wing vein | ectopic phenotype of mor⁵

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN is a suppressor of wing vein phenotype of N^Ax-16

(Di Stefano et al., 2011)

NOT Suppressor of

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN is a non-suppressor of wing vein | ectopic phenotype of Snr1^E1

(Curtis et al., 2011)

Other

Statement

Reference

Su(var)3-3[+]/Su(var)3-3^ΔN, brm² has wing vein | ectopic phenotype

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN, mor¹ has wing vein | ectopic phenotype

(Curtis et al., 2011)

Su(var)3-3[+]/Su(var)3-3^ΔN, polybromo^Δ86 has wing vein | ectopic phenotype

(Curtis et al., 2011)

Bap170^Δ65, Su(var)3-3[+]/Su(var)3-3^ΔN has wing vein | ectopic phenotype

(Curtis et al., 2011)

Su(var)3-3^ΔN, bam^hs.PO has female germline cyst phenotype

(Eliazer et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the ectopic venation phenotype observed in Su(var)3-3^ΔN homozygous adults is significantly increased upon combination with htt^int.

(Dietz et al., 2015)

Su(var)3-3^ΔN brm² double heterozygotes can have ectopic wing vein material distal to the posterior crossvein.

Su(var)3-3^ΔN mor¹ double heterozygotes often have ectopic wing vein material posterior to vein L5 and distal to the posterior crossvein.

100% of Su(var)3-3^ΔN mor³ double heterozygotes have ectopic wing vein material.

61% of Su(var)3-3^ΔN mor⁶ double heterozygotes have ectopic wing vein material.

(Curtis et al., 2011)

lid¹⁰⁴²⁴/+ partially suppresses the held-out wing phenotype and largely suppresses the extra wing vein phenotype which is seen in Su(var)3-3^ΔN homozygotes.

lid¹⁰⁴²⁴/+ partially suppresses the follicle cell defects seen in Su(var)3-3^ΔN females.

Su(H)⁰⁵/+ partially suppresses the oogenesis defects of Su(var)3-3^ΔN homozygous females.

The wing vein truncation phenotype which is seen in N^Ax-16/+ flies is suppressed by Su(var)3-3^ΔN/+.

(Di Stefano et al., 2011)

Expression of bam^hs.PO in Su(var)3-3^ΔN mutants results in the formation of multicellular cysts that contain branched fusomes indicating that Su(var)3-3^ΔN mutant germline cells can undergo differentiation and formation of multicellular cysts.

dpp^hr56/+ partially suppresses the Su(var)3-3^ΔN mutant supernumerary germline cell phenotype resulting in an increased number of germline cysts with branched fusomes and maturing egg chambers.

dpp^hr92/+ partially suppresses the Su(var)3-3^ΔN mutant supernumerary germline cell phenotype resulting in an increased number of germline cysts with branched fusomes and maturing egg chambers.

(Eliazer et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Su(var)3-3^ΔN is rescued by Scer\GAL4^C587/Su(var)3-3^UASp.Tag:HA

(Eliazer et al., 2014)

Su(var)3-3^ΔN is rescued by Scer\GAL4^C587/Su(var)3-3^UASt.Tag:HA

(Eliazer et al., 2014)

Partially rescued by

Su(var)3-3^ΔN is partially rescued by Su(var)3-3^UAS.cDa

(Eliazer et al., 2011)

Comments

(Eliazer et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

91358

w^*; PBac{WHr}Su(var)3-3^ΔN/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Lsd1^ΔN

(Shao et al., 2022, Eliazer et al., 2014, Eliazer et al., 2011)

P(lsd1)^ΔN

(Curtis et al., 2011)

Su(var)3-3^DeltaN

(Goupil et al., 2022)

Su(var)3-3^ΔN

dLsd1^ΔN

(Lepesant et al., 2020, Dietz et al., 2015, Di Stefano et al., 2011, Di Stefano et al., 2007)

lsd1^ΔN

(Lee and Spradling, 2014, Curtis et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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