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General Information
Symbol
Dmel\pydex147
Species
D. melanogaster
Name
FlyBase ID
FBal0248975
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, P{GawB}pydNP4400, results in a deletion encompassing a large region of pyd.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The nephrocytes of pydex147 homozygous stage 16 embryos are abnormally agglutinated, with electron-dense regions at membranes contact sites, thickening of the basement membrane, and complete absence of slit diaphragms; as in the wild-type controls, the only junctions between adjacent nephrocytes are found at the most external region contacting the basement membrane; however, the septate junction markers Dlg, Fas3 and Lgl localize at ectopic junctional complexes.

Nearly all pydex147 adults exhibit extra dorsal thoracic macrochaeta.

Embryos maternally and zygotically mutant for pydex180 exhibit 86% embryonic lethality.

Substantial embryonic lethality (43-58%) is found in flies from pydex147 mothers crossed with pydB12 fathers.

The ventral epidermis is intact in pydex147 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 26% of pydex147 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (45%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (29%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

The ventral epidermis is intact in pydB12/pydex147 maternal/zygotic mutant embryos and the CNS is not obviously hypertrophied. While approximately 17% of pydB12/pydex147 maternal/zygotic mutant embryos appear wild-type, most mutant embryos (64%) exhibit defects in head involution that result in defective head skeletons, whereas the most severe (19%) display complete failure of head involution, leading to a hole in the cuticle. In addition, some show minor defects in dorsal closure.

Over 90% of pydex147/Df(3R)p-XT103 flies display more than four dorsocentral macrochaetae.

Wings from pydex147/Df(3R)p-XT103 flies are broader compared to control wings. A 10% increase in the number of cells found between the L3 and L4 veins is observed. In third instar larval wing discs, mutant cells appear smaller, and there are 15% more cells per surface unit compared to controls.

Wings from pydex147/pydtam flies are broader compared to control wings.

Both pydex147/+ and pydex147/Df(3R)p-XT103 mutants display an increase in the number of ovarian niche cap cells.

An increase in the number of stem cells per ovariole is observed in pydex147/Df(3R)p-XT103 flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

pydtam/pydex147 has visible phenotype, non-enhanceable by N[+]/N55e11

NOT Enhancer of
Statement
Reference

pydex147/pyd[+] is a non-enhancer of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

NOT Suppressor of
Statement
Reference

pydex147/pyd[+] is a non-suppressor of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Phenotype Manifest In
Enhanced by
Statement
Reference

pydtam/pydex147 has wing phenotype, enhanceable by ex[+]/exe1

NOT Enhanced by
Statement
Reference

pydtam/pydex147 has wing phenotype, non-enhanceable by N[+]/N55e11

pydtam/pydex147 has wing phenotype, non-enhanceable by Ptendj189/Pten[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by Ptenc494/Pten[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by Su(dx)sp/Df(2L)Exel6006

pydtam/pydex147 has wing phenotype, non-enhanceable by Su(H)8/Su(H)[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by Su(dx)sp/Su(dx)[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by yps[+]/ypsJM2

pydtam/pydex147 has wing phenotype, non-enhanceable by Cdk43/Cdk4[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by Cdk4k06503/Cdk4[+]

pydtam/pydex147 has wing phenotype, non-enhanceable by Tor[+]/TorΔP

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

pydex147/pyd[+] is a non-enhancer of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

NOT Suppressor of
Statement
Reference

pydex147/pyd[+] is a non-suppressor of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Additional Comments
Genetic Interactions
Statement
Reference

The rough eye phenotype observed in adults expressing MagiScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU is not modified by combination with single copy of pydex147.

100% of N55e11/+; pydex147/Df(3R)p-XT103 flies display more than four dorsocentral macrochaetae.

The broader wings observed in pydex147/pydtam flies are not enhanced in a N55e11/+ background.

N55e11/+ suppresses the increased ovarian cap cell numbers observed in pydex147/+ mutants.

The increase in the number of ovarian cap cells observed in pydex147/+ animals is suppressed in a Su(dx)32/Su(dx)56 background.

The increase in the number of ovarian cap cells observed in pydex147/pydex147 animals is not suppressed in a Su(dx)32/Su(dx)56 background.

The increased number of dorsocentral macrochaetae in animals expressing Su(dx)Scer\UAS.cCa under the control of Scer\GAL4dpp.PU is enhanced in a pydex147/+ background.

Wings from pydex147/pydtam flies are broader compared to control wings, and this phenotype is enhanced in a exe1/+ background.

Wings from pydex147/pydtam flies are broader compared to control wings, and this phenotype is not modified in a Su(H)8/+, Su(dx)sp/+, ypsJM2/+, Cdk43/+, Cdk4k06503/+, TorΔP/+, Ptendj189/+, or Ptenc494/+ background.

Wings from pydex147/pydtam flies are broader compared to control wings, and this phenotype is not modified in a Su(dx)sp/Df(2L)Exel6006 background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The increased size of adult wings in pydex147/pydtam mutant flies is restored in animals expressing pydScer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is restored in animals expressing pydΔPDZ1.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is restored in animals expressing pydΔEx6.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is not restored in animals expressing pydΔPDZ2.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is not restored in animals expressing pydΔSH3.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is not restored in animals expressing pydΔGUK.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is not restored in animals expressing pydΔPro-Rich.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

The increased size of adult wings in pydex147/pydtam mutant flies is partially restored in animals expressing pydΔPDZ3.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)