The expression of
EcRdsRNA.UAS.cCa under the control of
Scer\GAL4Cg.PA blocks fat body cell migration into the pupal head.
The clonal expression of
EcRdsRNA.UAS.cCa under the control of
Scer\GAL4Act5C.PP significantly decreases the third instar salivary gland cell size in a cell autonomous manner, as compared to neighboring controls.
The expression of
EcRdsRNA.UAS.cCa under the control of
Scer\GAL4fkh.PH decreases the third instar salivary gland cell size, as compared to controls.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4GMR.PU results in a defective eye phenotype. 66.1% of flies show a slight phenotype, 25.6% show a moderate phenotype and 8.3% have a severe phenotype.
Animals expressing
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4c739 have defects in the mushroom body α/β lobes: the lobes may appear slim or underdeveloped, or the β lobes may be fused.
Animals expressing
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4let-7-C have defects in the mushroom body α/β lobes: the lobes may appear slim or occasionally underdeveloped, or the β lobes may be fused.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of either
Scer\GAL4ptc-559.1 or
Scer\GAL4bab1-Pgal4-2 in the somatic cells of the germarium (using the temperature sensitive
Scer\GAL80ts.αTub84B allele to restrict expression to the adult stage) results in supernumerary germline cells containing a single spectrosome at the expense of developing cysts.
Single cell somatic clones in the ovary expressing
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4Act.PU and induced during niche development resemble niche cells by their shape and their ability to anchor germline cells containing a single spectrosome (SSCs). The mutant germaria contain supernumerary SSCs compared to controls.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4da.Switch.PT in male flies in the presence of 50μg ml[-1] RU486 results in an increase in the median and maximum lifespan compared to controls (increase of 26% and 45% respectively). These males show a significant improvement in climbing ability at 4 weeks of age compared to controls. In the presence of 100μg ml[-1] RU486 there is no effect on the median lifespan and maximum lifespan is only increased by 17% in males expressing
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4da.Switch.PT. In the presence of 200μg ml[-1] RU486 the median lifespan of these males is 14% lower than that of controls.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4Switch1.106 in male flies in the presence of RU486 increases their mean and median lifespans over the full range of RU486 concentrations tested.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4da.Switch.PT in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner. A few days after starting RU4896 treatment, the number of eggs laid per female is lower than that laid by control females and is RU486 dose-dependent. Most of the eggs do not develop and the eggs show a number of defects including wide, branched dorsal appendages and thin eggshells. Ovaries of the treated females only show defects from stage 10 of oogenesis, notably delayed border cell migration and abnormal dorsal appendage morphogenesis.
Expression of
EcRdsRNA.Scer\UAS.cCa under the control of
Scer\GAL4ppk.1.9 results in a significant reduction in the mean density of the dendritic branches of the ddaC dendritic arborisation neurons compared to controls.
