Open Close
General Information
Symbol
Dmel\EcRdsRNA.UAS.cCa
Species
D. melanogaster
Name
FlyBase ID
FBal0249021
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-EcR-RNAi, UAS-EcR RNAi, UAS-IR-EcR, UAS-EcR-RNAi104, EcR RNAi, EcRi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt sequences drive expression of a 663bp fragment of EcR (nts 2354- 3017 of GenBank Accession M74048) organised as an inverted repeat.
Construct: A 663bp fragment corresponding to the EcR-B1 cDNA sequence is cloned into pUAST, first in a reverse orientation and then in a forward orientation
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of EcRdsRNA.UAS.cCa under the control of Scer\GAL4Cg.PA blocks fat body cell migration into the pupal head. The clonal expression of EcRdsRNA.UAS.cCa under the control of Scer\GAL4Act5C.PP significantly decreases the third instar salivary gland cell size in a cell autonomous manner, as compared to neighboring controls. The expression of EcRdsRNA.UAS.cCa under the control of Scer\GAL4fkh.PH decreases the third instar salivary gland cell size, as compared to controls.
Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4GMR.PU results in a defective eye phenotype. 66.1% of flies show a slight phenotype, 25.6% show a moderate phenotype and 8.3% have a severe phenotype.
Photoreceptors expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4GMR.PF display apical membrane maturation defects.
Animals expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4c739 have defects in the mushroom body α/β lobes: the lobes may appear slim or underdeveloped, or the β lobes may be fused. Animals expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4let-7-C have defects in the mushroom body α/β lobes: the lobes may appear slim or occasionally underdeveloped, or the β lobes may be fused.
Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4Tab2-201Y results in a suppression of the normal pruning of mushroom body gamma neurons. Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4ppk.1.9 results in severe dendrite severing defects in the ddaC dorsal dendritic arborising neurons at 18 hours after puparium formation.
Expression of EcRdsRNA.Scer\UAS.cCa under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4bab1-Pgal4-2 in the somatic cells of the germarium (using the temperature sensitive Scer\GAL80ts.αTub84B allele to restrict expression to the adult stage) results in supernumerary germline cells containing a single spectrosome at the expense of developing cysts. Single cell somatic clones in the ovary expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4Act.PU and induced during niche development resemble niche cells by their shape and their ability to anchor germline cells containing a single spectrosome (SSCs). The mutant germaria contain supernumerary SSCs compared to controls.
Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4da.Switch.PT in male flies in the presence of 50μg ml[-1] RU486 results in an increase in the median and maximum lifespan compared to controls (increase of 26% and 45% respectively). These males show a significant improvement in climbing ability at 4 weeks of age compared to controls. In the presence of 100μg ml[-1] RU486 there is no effect on the median lifespan and maximum lifespan is only increased by 17% in males expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4da.Switch.PT. In the presence of 200μg ml[-1] RU486 the median lifespan of these males is 14% lower than that of controls. Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4Switch1.106 in male flies in the presence of RU486 increases their mean and median lifespans over the full range of RU486 concentrations tested. Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4da.Switch.PT in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner. A few days after starting RU4896 treatment, the number of eggs laid per female is lower than that laid by control females and is RU486 dose-dependent. Most of the eggs do not develop and the eggs show a number of defects including wide, branched dorsal appendages and thin eggshells. Ovaries of the treated females only show defects from stage 10 of oogenesis, notably delayed border cell migration and abnormal dorsal appendage morphogenesis.
Expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4ppk.1.9 results in a significant reduction in the mean density of the dendritic branches of the ddaC dendritic arborisation neurons compared to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
EcRdsRNA.UAS.cCa, Scer\GAL4GMR.PU has visible phenotype, enhanceable by Brms1[+]/Brms1050-2
Suppressed by
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Scer\GAL4Sgs3.PD-mediated expression of EcRdsRNA.Scer\UAS.cCa in a Utx1 background significantly reduces the proportion of animals surviving to head eversion, compared to Utx1 animals alone.
Brms1050-2/+ enhances the severity of the defective eye phenotype caused expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4GMR.PU. 8.9% of flies show a slight phenotype, 46.6% show a moderate phenotype and 44.5% have a severe phenotype.
Co-expression of hdcScer\UAS.cSa partially suppresses the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4ppk.1.9 at 18 hours after puparium formation.
The deleterious effect on female lifespan of expression of EcRdsRNA.Scer\UAS.cCa under the control of Scer\GAL4da.Switch.PT in the presence of RU486 is partially suppressed by ovoD1.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
EcRIR.Scer\UAS
EcRdsRNA.Scer\UAS.cCa
EcRdsRNA.Scer\UAS
EcRdsRNA.UAS.cCa
Name Synonyms
Secondary FlyBase IDs
  • FBal0189818
  • FBal0189813
References (25)