Allele Dmel\EcR^{dsRNA.Scer\UAS.cCa}

General Information
Symbol	Dmel\EcR^{dsRNA.Scer\UAS.cCa}	Species	D. melanogaster
Name		FlyBase ID	FBal0249021
Feature type	allele	Associated gene	Dmel\EcR

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Colombani et al., 2005, Colombani et al., 2005) in vitro construct - RNAi (Schubiger et al., 2005, Colombani et al., 2005, Colombani et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of a 663bp fragment of EcR (nts 2354- 3017 of GenBank Accession M74048) organised as an inverted repeat. (Colombani et al., 2005) Construct: Scer\UAS sequences drive expression of a 663bp fragment of EcR (nts 2354-3017 of GenBank Accession M74048) organised as an inverted repeat. (Colombani et al., 2005) Construct: A 663bp fragment corresponding to the EcR-B1 cDNA sequence is cloned into pUAST, first in a reverse orientation and then in a forward orientation (Schubiger et al., 2005)
Carried in construct	P{UAS-EcR-RNAi} (Mou et al., 2012, Costantino et al., 2008, Chawla and Sokol, 2012, Gradilla et al., 2011, Tricoire et al., 2009, Colombani et al., 2005, Colombani et al., 2005, Varghese and Cohen, 2007, Slaidina et al., 2009, Schubiger et al., 2005, Ninov et al., 2007, Tricoire et al., 2009, König et al., 2011, Fichelson et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^phm.PU (Gradilla et al., 2011) long lived \| male \| RU486 conditional, with Scer\GAL4^Switch1.106 (Tricoire et al., 2009) short lived \| female \| RU486 conditional, with Scer\GAL4^da.Switch.PT (Tricoire et al., 2009)
Phenotype Manifest In
	female germline cell \| somatic clone \| supernumerary, with Scer\GAL4^Act.PU (König et al., 2011) germline cell \| supernumerary \| heat sensitive, with Scer\GAL4^bab1-Pgal4-2, Scer\GAL80^ts.αTub84B (König et al., 2011) germline cell \| supernumerary \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (König et al., 2011) photoreceptor, with Scer\GAL4^GMR.PF (Fichelson et al., 2012)
Detailed Description
	Statement Reference Photoreceptors expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[GMR.PF] display apical membrane maturation defects. (Fichelson et al., 2012) Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of either Scer\GAL4[ptc-559.1] or Scer\GAL4[bab1-Pgal4-2] in the somatic cells of the germarium (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to restrict expression to the adult stage) results in supernumerary germline cells containing a single spectrosome at the expense of developing cysts. Single cell somatic clones in the ovary expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[Act.PU] and induced during niche development resemble niche cells by their shape and their ability to anchor germline cells containing a single spectrosome (SSCs). The mutant germaria contain supernumerary SSCs compared to controls. (König et al., 2011) Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT] in male flies in the presence of 50μg ml[-1] RU486 results in an increase in the median and maximum lifespan compared to controls (increase of 26% and 45% respectively). These males show a significant improvement in climbing ability at 4 weeks of age compared to controls. In the presence of 100μg ml[-1] RU486 there is no effect on the median lifespan and maximum lifespan is only increased by 17% in males expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT]. In the presence of 200μg ml[-1] RU486 the median lifespan of these males is 14% lower than that of controls. Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[Switch1.106] in male flies in the presence of RU486 increases their mean and median lifespans over the full range of RU486 concentrations tested. Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT] in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner. A few days after starting RU4896 treatment, the number of eggs laid per female is lower than that laid by control females and is RU486 dose-dependent. Most of the eggs do not develop and the eggs show a number of defects including wide, branched dorsal appendages and thin eggshells. Ovaries of the treated females only show defects from stage 10 of oogenesis, notably delayed border cell migration and abnormal dorsal appendage morphogenesis. (Tricoire et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The deleterious effect on female lifespan of expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT] in the presence of RU486 is partially suppressed by ovo[D1]. (Tricoire et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	EcR^{dsRNA.Scer\UAS.cCa} EcR^{dsRNA.Scer\UAS} EcR^IR.Scer\UAS
Name Synonym
Secondary FlyBase IDs
	FBal0189813 FBal0189818
References ( 14 )
Research paper	Chawla and Sokol, 2012, Development 139(10): 1788--1797 Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. [FBrf0218077] Fichelson et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(20): 7893--7898 Orthodenticle and Kruppel homolog 1 regulate Drosophila photoreceptor maturation. [FBrf0218313] Mou et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(8): 2949--2954 Control of target gene specificity during metamorphosis by the steroid response gene E93. [FBrf0217473] Gradilla et al., 2011, Genetics 189(3): 871--883 Isoform-Specific Regulation of a Steroid Hormone Nuclear Receptor by an E3 Ubiquitin Ligase in Drosophila melanogaster. [FBrf0216551] König et al., 2011, EMBO J. 30(8): 1549--1562 Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation. [FBrf0213531] Slaidina et al., 2009, Dev. Cell 17(6): 874--884 A Drosophila insulin-like peptide promotes growth during nonfeeding states. [FBrf0209691] Tricoire et al., 2009, Mech. Ageing Dev. 130(8): 547--552 The steroid hormone receptor EcR finely modulates Drosophila lifespan during adulthood in a sex-specific manner. [FBrf0208400] Costantino et al., 2008, PLoS Genet. 4(6): e1000102 A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila. [FBrf0205257] Ninov et al., 2007, Development 134(2): 367--379 Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. [FBrf0194691] Varghese and Cohen, 2007, Genes Dev. 21(18): 2277--2282 microRNA miR-14 acts to modulate a positive autoregulatory loop controlling steroid hormone signaling in Drosophila. [FBrf0201357] Colombani et al., 2005, Science 310(5748): 667--670 Antagonistic actions of ecdysone and insulins determine final size in Drosophila. [FBrf0190605] Schubiger et al., 2005, Development 132(23): 5239--5248 Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing. [FBrf0190319]
Supplementary material	Tricoire et al., 2009, Mech. Ageing Dev. 130(8): Supplementary information: Materials and methods. [FBrf0212919] Colombani et al., 2005, Science 310(5748): Antagonistic actions of ecdysone and insulins determine final size in Drosophila. [FBrf0191480]

Allele Dmel\EcRdsRNA.Scer\UAS.cCa

Allele Dmel\EcR^{dsRNA.Scer\UAS.cCa}