|Feature type||allele||Associated gene||Dmel\EcR|
|Mutagen||in vitro construct - RNAi, in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Construct: Scer\UAS sequences drive expression of a 663bp fragment of EcR (nts 2354- 3017 of GenBank Accession M74048) organised as an inverted repeat.
Construct: Scer\UAS sequences drive expression of a 663bp fragment of EcR (nts 2354-3017 of GenBank Accession M74048) organised as an inverted repeat.
Construct: A 663bp fragment corresponding to the EcR-B1 cDNA sequence is cloned into pUAST, first in a reverse orientation and then in a forward orientation
|Carried in construct|
(Mou et al., 2012, Costantino et al., 2008, Chawla and Sokol, 2012, Gradilla et al., 2011, Tricoire et al., 2009, Colombani et al., 2005, Colombani et al., 2005, Varghese and Cohen, 2007, Slaidina et al., 2009, Schubiger et al., 2005, Ninov et al., 2007, Tricoire et al., 2009, König et al., 2011, Fichelson et al., 2012)
|Phenotype Manifest In|
Photoreceptors expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[GMR.PF] display apical membrane maturation defects.
Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of either Scer\GAL4[ptc-559.1] or Scer\GAL4[bab1-Pgal4-2] in the somatic cells of the germarium (using the temperature sensitive Scer\GAL80[ts.αTub84B] allele to restrict expression to the adult stage) results in supernumerary germline cells containing a single spectrosome at the expense of developing cysts. Single cell somatic clones in the ovary expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[Act.PU] and induced during niche development resemble niche cells by their shape and their ability to anchor germline cells containing a single spectrosome (SSCs). The mutant germaria contain supernumerary SSCs compared to controls.
Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT] in male flies in the presence of 50μg ml[-1] RU486 results in an increase in the median and maximum lifespan compared to controls (increase of 26% and 45% respectively). These males show a significant improvement in climbing ability at 4 weeks of age compared to controls. In the presence of 100μg ml[-1] RU486 there is no effect on the median lifespan and maximum lifespan is only increased by 17% in males expressing EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT]. In the presence of 200μg ml[-1] RU486 the median lifespan of these males is 14% lower than that of controls. Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[Switch1.106] in male flies in the presence of RU486 increases their mean and median lifespans over the full range of RU486 concentrations tested. Expression of EcR[dsRNA.Scer\UAS.cCa] under the control of Scer\GAL4[da.Switch.PT] in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner. A few days after starting RU4896 treatment, the number of eggs laid per female is lower than that laid by control females and is RU486 dose-dependent. Most of the eggs do not develop and the eggs show a number of defects including wide, branched dorsal appendages and thin eggshells. Ovaries of the treated females only show defects from stage 10 of oogenesis, notably delayed border cell migration and abnormal dorsal appendage morphogenesis.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 3 )|
|Secondary FlyBase IDs|
|References ( 14 )|