FlyBase Allele Report: Dmel\EcR[dsRNA.UAS.cCa]

General Information

Symbol

Dmel\EcR^{dsRNA.UAS.cCa}

Species

D. melanogaster

Name

FlyBase ID

FBal0249021

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR-RNAi}

Also Known As

UAS-EcR-RNAi, UAS-EcR RNAi, EcR RNAi, UAS-IR-EcR, UAS-EcR-RNAi¹⁰⁴, UAS-EcR^RNAi, EcRi, EcR-RNAi

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Colombani et al., 2005, Colombani et al., 2005, Schubiger et al., 2005)

in vitro construct - regulatory fusion

(Colombani et al., 2005, Colombani et al., 2005)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-EcR-RNAi}

Cytology

Nature of the lesion

Statement

Reference

UASt sequences drive expression of a 663bp fragment of EcR (nts 2354- 3017 of GenBank Accession M74048) organised as an inverted repeat.

(Colombani et al., 2005)

Construct: A 663bp fragment corresponding to the EcR-B1 cDNA sequence is cloned into pUAST, first in a reverse orientation and then in a forward orientation

(Schubiger et al., 2005)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

cell migration defective | pupal stage, with Scer\GAL4^Cg.PA

(Okamoto et al., 2018)

decreased cell size | cell autonomous | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Okamoto et al., 2018)

decreased cell size | third instar larval stage, with Scer\GAL4^fkh.PH

(Okamoto et al., 2018)

decreased fecundity | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

lethal, with Scer\GAL4^phm.PU

(Gradilla et al., 2011)

long lived | male | RU486 conditional, with Scer\GAL4^Switch1.106

(Tricoire et al., 2009)

neuroanatomy defective, with Scer\GAL4^c739

(Kucherenko et al., 2012)

neuroanatomy defective, with Scer\GAL4^let7C

(Kucherenko et al., 2012)

neuroanatomy defective, with Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012, Ou et al., 2008)

neuroanatomy defective, with Scer\GAL4^Tab2-201Y

(Loncle and Williams, 2012)

short lived | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Tricoire et al., 2009)

visible, with Scer\GAL4^GMR.PU

(Song et al., 2013)

Phenotype Manifest In

adult mushroom body beta-lobe, with Scer\GAL4^c739

(Kucherenko et al., 2012)

adult mushroom body beta-lobe, with Scer\GAL4^let7C

(Kucherenko et al., 2012)

dendrite, with Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012, Ou et al., 2008)

dendrite, with Scer\GAL4^Tab2-201Y

(Loncle and Williams, 2012)

embryonic/larval salivary gland | cell autonomous | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Okamoto et al., 2018)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^fkh.PH

(Okamoto et al., 2018)

eye, with Scer\GAL4^GMR.PU

(Song et al., 2013)

fat cell | pupal stage, with Scer\GAL4^Cg.PA

(Okamoto et al., 2018)

female germline cell | somatic clone | supernumerary, with Scer\GAL4^Act.PU

(König et al., 2011)

gamma Kenyon cell, with Scer\GAL4^Tab2-201Y

(Loncle and Williams, 2012)

germline cell | supernumerary | heat sensitive, with Scer\GAL4^bab1-Pgal4-2, Scer\GAL80^ts.αTub84B

(König et al., 2011)

germline cell | supernumerary | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(König et al., 2011)

head | pupal stage, with Scer\GAL4^Cg.PA

(Okamoto et al., 2018)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012, Ou et al., 2008)

lobe system of adult mushroom body, with Scer\GAL4^c739

(Kucherenko et al., 2012)

lobe system of adult mushroom body, with Scer\GAL4^let7C

(Kucherenko et al., 2012)

photoreceptor, with Scer\GAL4^GMR.PF

(Fichelson et al., 2012)

Detailed Description

Statement

Reference

The expression of EcR^{dsRNA.UAS.cCa} under the control of Scer\GAL4^Cg.PA blocks fat body cell migration into the pupal head.

The clonal expression of EcR^{dsRNA.UAS.cCa} under the control of Scer\GAL4^Act5C.PP significantly decreases the third instar salivary gland cell size in a cell autonomous manner, as compared to neighboring controls.

The expression of EcR^{dsRNA.UAS.cCa} under the control of Scer\GAL4^fkh.PH decreases the third instar salivary gland cell size, as compared to controls.

(Okamoto et al., 2018)

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^GMR.PU results in a defective eye phenotype. 66.1% of flies show a slight phenotype, 25.6% show a moderate phenotype and 8.3% have a severe phenotype.

(Song et al., 2013)

Photoreceptors expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^GMR.PF display apical membrane maturation defects.

(Fichelson et al., 2012)

Animals expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^c739 have defects in the mushroom body α/β lobes: the lobes may appear slim or underdeveloped, or the β lobes may be fused.

Animals expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^let-7-C have defects in the mushroom body α/β lobes: the lobes may appear slim or occasionally underdeveloped, or the β lobes may be fused.

(Kucherenko et al., 2012)

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^Tab2-201Y results in a suppression of the normal pruning of mushroom body gamma neurons.

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^ppk.1.9 results in severe dendrite severing defects in the ddaC dorsal dendritic arborising neurons at 18 hours after puparium formation.

(Loncle and Williams, 2012)

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^bab1-Pgal4-2 in the somatic cells of the germarium (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to restrict expression to the adult stage) results in supernumerary germline cells containing a single spectrosome at the expense of developing cysts.

Single cell somatic clones in the ovary expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^Act.PU and induced during niche development resemble niche cells by their shape and their ability to anchor germline cells containing a single spectrosome (SSCs). The mutant germaria contain supernumerary SSCs compared to controls.

(König et al., 2011)

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^da.Switch.PT in male flies in the presence of 50μg ml[-1] RU486 results in an increase in the median and maximum lifespan compared to controls (increase of 26% and 45% respectively). These males show a significant improvement in climbing ability at 4 weeks of age compared to controls. In the presence of 100μg ml[-1] RU486 there is no effect on the median lifespan and maximum lifespan is only increased by 17% in males expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^da.Switch.PT. In the presence of 200μg ml[-1] RU486 the median lifespan of these males is 14% lower than that of controls.

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^Switch1.106 in male flies in the presence of RU486 increases their mean and median lifespans over the full range of RU486 concentrations tested.

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 in female flies decreases lifespan in a RU486 dose-dependent manner. A few days after starting RU4896 treatment, the number of eggs laid per female is lower than that laid by control females and is RU486 dose-dependent. Most of the eggs do not develop and the eggs show a number of defects including wide, branched dorsal appendages and thin eggshells. Ovaries of the treated females only show defects from stage 10 of oogenesis, notably delayed border cell migration and abnormal dorsal appendage morphogenesis.

(Tricoire et al., 2009)

Expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^ppk.1.9 results in a significant reduction in the mean density of the dendritic branches of the ddaC dendritic arborisation neurons compared to controls.

(Ou et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

EcR^{dsRNA.UAS.cCa}, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Brms1[+]/Brms1^050-2

(Song et al., 2013)

Suppressed by

Statement

Reference

EcR^{dsRNA.UAS.cCa}, Scer\GAL4^ppk.1.9 has neuroanatomy defective phenotype, suppressible | partially by hdc^UAS.cSa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Enhancer of

Statement

Reference

EcR^{dsRNA.UAS.cCa}/Scer\GAL4^Sgs3.PD is an enhancer of lethal - all die during pupal stage phenotype of Utx¹

(Denton et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

EcR^{dsRNA.UAS.cCa}, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Brms1^050-2

(Song et al., 2013)

Suppressed by

Statement

Reference

EcR^{dsRNA.UAS.cCa}, Scer\GAL4^ppk.1.9 has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by hdc^UAS.cSa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

EcR^{dsRNA.UAS.cCa}, Scer\GAL4^ppk.1.9 has dendrite phenotype, suppressible | partially by hdc^UAS.cSa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Sgs3.PD-mediated expression of EcR^{dsRNA.Scer\UAS.cCa} in a Utx¹ background significantly reduces the proportion of animals surviving to head eversion, compared to Utx¹ animals alone.

(Denton et al., 2013)

Brms1^050-2/+ enhances the severity of the defective eye phenotype caused expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^GMR.PU. 8.9% of flies show a slight phenotype, 46.6% show a moderate phenotype and 44.5% have a severe phenotype.

(Song et al., 2013)

Co-expression of hdc^Scer\UAS.cSa partially suppresses the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^ppk.1.9 at 18 hours after puparium formation.

(Loncle and Williams, 2012)

The deleterious effect on female lifespan of expression of EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^da.Switch.PT in the presence of RU486 is partially suppressed by ovo^D1.

(Tricoire et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9326

w¹¹¹⁸; P{UAS-EcR-RNAi}97

9327

w¹¹¹⁸; P{UAS-EcR-RNAi}104

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

EcR^IR.Scer\UAS

EcR^{dsRNA.Scer\UAS.cCa}

EcR^{dsRNA.Scer\UAS}

EcR^{dsRNA.UAS.cCa}

Name Synonyms

Secondary FlyBase IDs

FBal0189818
FBal0189813

References (30)

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