FlyBase Allele Report: Dmel\ImpL2[s.UAS]

General Information

Symbol

Dmel\ImpL2^s.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0249386

Feature type

allele

Associated gene

Dmel\ImpL2

Associated Insertion(s)

Carried in Construct

P{UAS-ImpL2.strong}

Also Known As

UAS-s.ImpL2, UAS-sImp-L2

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Honegger et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-ImpL2.strong}

Cytology

Description

UASt regulatory sequences drive expression of ImpL2 lacking the first exon (containing three upstream open reading frames) of the ImpL2-RB transcript.

(Honegger et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ImpL2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^GMR.PF

(Honegger et al., 2008)

atrophy, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

increased cell death, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

lethal, with Scer\GAL4^Act5C.PI

(Honegger et al., 2008)

lethal, with Scer\GAL4^arm.PS

(Honegger et al., 2008)

lethal, with Scer\GAL4^ppl.PP

(Honegger et al., 2008)

long lived, with Scer\GAL4^da.Switch.PT

(Paik et al., 2012)

semi-fertile, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

Phenotype Manifest In

egg, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

egg chamber, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

eye, with Scer\GAL4^GMR.PF

(Honegger et al., 2008)

fat body, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

ovary, with Scer\GAL4^byn.n2

(Figueroa-Clarevega and Bilder, 2015)

Detailed Description

Statement

Reference

Expression of ImpL2^s.Scer\UAS in the hindgut under the control of Scer\GAL4^byn.n2 results in a substantial reduction in ovarian size, which is accompanied by apoptotic egg chambers, loss of mature eggs, and reduced fertility. Hindgut driven ImpL2^s.Scer\UAS-expression also reduces fat body size and induces lipid droplet enlargement.

(Figueroa-Clarevega and Bilder, 2015)

Overexpression of ImpL2^s.Scer\UAS under the control of Scer\GAL4^da.Switch.PT significantly extends the lifespan compared to control flies.

(Paik et al., 2012)

Ubiquitous overexpression of ImpL2^s.Scer\UAS under the control of Scer\GAL4^Act5C.PI leads to lethality.

Ubiquitous overexpression of ImpL2^s.Scer\UAS under the control of Scer\GAL4^arm.PS leads to lethality.

Overexpression of ImpL2^s.Scer\UAS driven by Scer\GAL4^GMR.PF results in a strong reduction of eye size. The body weight of ImpL2^s.Scer\UAS-overexpressing flies is reduced compared with controls. The development of Scer\GAL4^GMR.PF-driven ImpL2^s.Scer\UAS-overexpressing males is delayed by one day.

Driving ImpL2^s.Scer\UAS by Scer\GAL4^ppl.PP results in lethality.

(Honegger et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ImpL2^s.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

ImpL2^s.UAS, Scer\GAL4^ppl.PP is a suppressor of lethal phenotype of Ilp2^UAS.cBa, Scer\GAL4^ppl.PP

(Honegger et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

ImpL2^s.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of InR^UAS.cHa, Scer\GAL4^GMR.PF

(Honegger et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

ImpL2^s.Scer\UAS completely reverses the eye overgrowth resulting from the overexpression of InR^Scer\UAS.cHa driven by Scer\GAL4^GMR.PF.

Co-expression of Ilp2^Scer\UAS.cBa with ImpL2^s.Scer\UAS under the control of Scer\GAL4^ppl.PP suppresses the lethality caused by the overexpression of the individual Ilp2^Scer\UAS.cBa or ImpL2^s.Scer\UAS transgenes.

(Honegger et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ImpL2^s.Scer\UAS

ImpL2^s.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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