Allele Dmel\W^Scer\UAS.cUa

General Information
Symbol	Dmel\W^Scer\UAS.cUa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Unknown	FlyBase ID	FBal0249394
Feature type	allele	Associated gene	Dmel\W

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Shukla and Tapadia, 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of W. (Shukla and Tapadia, 2011)
Carried in construct	P{UAS-W.U} (Keene et al., 2011, Kanao et al., 2010, Shukla and Tapadia, 2011, Schoenmann et al., 2010, Charroux and Royet, 2009, Kim et al., 2010, Protzer et al., 2009, Broderick et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^Hml.Δ (Charroux and Royet, 2009) increased cell death, with Scer\GAL4^Hml.Δ (Charroux and Royet, 2009) increased cell death \| larval stage, with Scer\GAL4^Hml.Δ (Broderick et al., 2012) lethal, with Scer\GAL4^c42 (Shukla and Tapadia, 2011) lethal, with Scer\GAL4^Hml.Δ (Broderick et al., 2012) partially lethal - majority die \| pupal stage, with Scer\GAL4^Hml.Δ (Charroux and Royet, 2009) visible, with Scer\GAL4^GMR.PF (Kanao et al., 2010) visible, with Scer\GAL4^GMR.PU (Protzer et al., 2009)
Phenotype Manifest In
	embryonic/larval lymph gland \| larval stage, with Scer\GAL4^Hml.Δ (Broderick et al., 2012) eye, with Scer\GAL4^GMR.PF (Kanao et al., 2010) eye, with Scer\GAL4^GMR.PU (Protzer et al., 2009) plasmatocyte, with Scer\GAL4^Hml.Δ (Charroux and Royet, 2009)
Detailed Description
	Statement Reference The lymph glands of larvae expressing W[Scer\UAS.cUa] under the control of Scer\GAL4[Hml.Δ] stain with acridine orange, indicating cell death. TUNEL labelling indicates that the cell death is due to apoptosis. (Broderick et al., 2012) Expression of W[Scer\UAS.cUa] in all circadian clock neurons (Scer\GAL4[tim.PE]) has no effect on light avoidance. (Keene et al., 2011) Expression of W[Scer\UAS.cUa] under the control Scer\GAL4[c42] results in lethality during embryonic and first instar larval stages. (Shukla and Tapadia, 2011) Expression of W[Scer\UAS.cUa] under the control of Scer\GAL4[GMR.PF] results in eye degeneration. (Kanao et al., 2010) Expression of W[Scer\UAS.cUa] under the control of Scer\GAL4[Hml.Δ] affects the level of plasmatocytes in third instar larvae, with these larvae possessing less than 2% of non-apoptotic plasmatocytes. A significant percentage of these larvae reach pharate adult stage and emerge as phenotypically normal adults. Such adults contain very few remaining plasmatocytes compared to controls. These mutants reach the pupal stage in the same time as wild-type controls and do not contain melanotic tunours or any other obvious phenotypes. Scer\GAL4[Hml.Δ], W[Scer\UAS.cUa] flies show a clear increased susceptibility to infection by both Gram-positive and Gram-negative bacteria. Approximately 67% of plasmatocyte-depleted Scer\GAL4[Hml.Δ], W[Scer\UAS.cUa] flies succumb to infection by Enterococcus faecalis within 68 hours of infection. This lethality is correlated with a faster and higher bacterial growth in plasmatocyte depleted Scer\GAL4[Hml.Δ], W[Scer\UAS.cUa] flies compared to wild-type. Similar susceptibility is observed when the flies are infected with fungi. Approximately 55% of Scer\GAL4[Hml.Δ], W[Scer\UAS.cUa] larvae die during post-embryonic development, more specifically, at the pupal stage. This pupal partial lethality is completely suppressed by antibiotic treatment. (Charroux and Royet, 2009) Expression of W[Scer\UAS.cUa] under the control of Scer\GAL4[GMR.PU] results in adults with small, rough eyes. (Protzer et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has visible phenotype, non-enhanceable by α4GT1^{dsRNA.Scer\UAS.P\T}, Scer\GAL4^GMR.PU (Protzer et al., 2009) Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has visible phenotype, non-enhanceable by α4GT1^EP797, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^Hml.Δ, W^Scer\UAS.cUa has increased cell death \| larval stage phenotype, suppressible by BacA\p35^Scer\UAS.cUa, Scer\GAL4^Hml.Δ (Broderick et al., 2012) Scer\GAL4^Hml.Δ, W^Scer\UAS.cUa has increased cell death phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Hml.Δ (Charroux and Royet, 2009) Scer\GAL4^Hml.Δ, W^Scer\UAS.cUa has partially lethal - majority die \| pupal stage phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Hml.Δ (Charroux and Royet, 2009)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has visible phenotype, non-suppressible by α4GT1^{dsRNA.Scer\UAS.P\T}, Scer\GAL4^GMR.PU (Protzer et al., 2009) Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has visible phenotype, non-suppressible by α4GT1^EP797, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Other
Statement Reference Scer\GAL4^Rh5.PT, W^Scer\UAS.cUa, rpr^Scer\UAS.C has phototaxis defective \| larval stage phenotype (Keene et al., 2011) Scer\GAL4^tim.PE, W^Scer\UAS.cUa, rpr^Scer\UAS.C has phototaxis defective \| larval stage phenotype (Keene et al., 2011)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has eye phenotype, non-enhanceable by α4GT1^{dsRNA.Scer\UAS.P\T}, Scer\GAL4^GMR.PU (Protzer et al., 2009) Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has eye phenotype, non-enhanceable by α4GT1^EP797, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Suppressed by
Statement Reference Scer\GAL4^Hml.Δ, W^Scer\UAS.cUa has embryonic/larval lymph gland \| larval stage phenotype, suppressible by BacA\p35^Scer\UAS.cUa, Scer\GAL4^Hml.Δ (Broderick et al., 2012) Scer\GAL4^Hml.Δ, W^Scer\UAS.cUa has plasmatocyte phenotype, suppressible by BacA\p35^Scer\UAS.cHa, Scer\GAL4^Hml.Δ (Charroux and Royet, 2009)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has eye phenotype, non-suppressible by α4GT1^{dsRNA.Scer\UAS.P\T}, Scer\GAL4^GMR.PU (Protzer et al., 2009) Scer\GAL4^GMR.PU, W^Scer\UAS.cUa has eye phenotype, non-suppressible by α4GT1^EP797, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of both W[Scer\UAS.cUa] and rpr[Scer\UAS.C] under the control of Scer\GAL4[Rh5.PT] abolishes light avoidance at the larval stage. However, expression under the control of Scer\GAL4[Rh6.PD] does not affect light avoidance. Expression of both W[Scer\UAS.cUa] and rpr[Scer\UAS.C] efficiently ablates all circadian clock neurons (Scer\GAL4[tim.PE]), leaving larvae with a seriously disrupted light avoidance phenotype. This phenotype is no different in LD or DD conditions. Expression of both W[Scer\UAS.cUa] and rpr[Scer\UAS.C] efficiently ablates the lateral neurons (Scer\GAL4[P2.4.Pdf]) but has no effect on light avoidance. (Keene et al., 2011) Co-expression of either α4GT1[EP797] or α4GT1[dsRNA.Scer\UAS.P\T] has no effect on the adult eye phenotype caused by expression of W[Scer\UAS.cUa] under the control of Scer\GAL4[GMR.PU]. (Protzer et al., 2009)
Xenogenetic Interactions
Statement Reference Co-expression of BacA\p35[Scer\UAS.cUa] suppresses the cell death seen in the lymph glands of larvae expressing W[Scer\UAS.cUa] under the control of Scer\GAL4[Hml.Δ]. (Broderick et al., 2012) Expression of BacA\p35[Scer\UAS.cHa] rescues the pupal partial lethality found upon expression of W[Scer\UAS.cUa] under the control of Scer\GAL4[Hml.Δ] through blocking plasmatocyte apoptosis. (Charroux and Royet, 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	W^Scer\UAS.cUa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs

References ( 8 )
Research paper	Broderick et al., 2012, PLoS ONE 7(9): e44567 Drosophila ninjurin a induces nonapoptotic cell death. [FBrf0219544] Keene et al., 2011, J. Neurosci. 31(17): 6527--6534 Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment. [FBrf0213587] Shukla and Tapadia, 2011, Europ. J. Cell Biol. 90(1): 72--80 Differential localization and processing of apoptotic proteins in Malpighian tubules of Drosophila during metamorphosis. [FBrf0212365] Kanao et al., 2010, Hum. Mol. Genet. 19(19): 3747--3758 Activation of FoxO by LRRK2 induces expression of proapoptotic proteins and alters survival of postmitotic dopaminergic neuron in Drosophila. [FBrf0211787] Kim et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(18): 8440--8445 Drosophila TRPA1 channel mediates chemical avoidance in gustatory receptor neurons. [FBrf0210738] Schoenmann et al., 2010, J. Neurosci. 30(18): 6375--6386 Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. [FBrf0210748] Charroux and Royet, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(24): 9797--9802 Elimination of plasmatocytes by targeted apoptosis reveals their role in multiple aspects of the Drosophila immune response. [FBrf0208147] Protzer et al., 2009, Cell Tissue Res. 336(1): 149--158 Drosophila alpha-1,4-glycosyltransferase (alpha-4GT1) inhibits reaper-mediated apoptosis in the eye. [FBrf0215427]

Allele Dmel\WScer\UAS.cUa

Allele Dmel\W^Scer\UAS.cUa