Excision of P{EPgy2}CASKEY07081, resulting in a 2624 bp deletion of 5'UTR and the entire first coding exon of CASK. This first exon contains the translational start site an the first 20 codons of the ORF.
neurite | larval stage (with Df(3R)cakiX-313)
neurite | larval stage (with Df(3R)X-307)
The neuromuscular junction of mutant third instar larvae is altered compared to wild type. The number of type Is boutons/muscle area is normal, but the bouton area is reduced compared to wild type. For type Ib boutons, bouton number/muscle area is increased and bouton area is reduced.
Mutant larvae are deficient in the ability to associate an odour with a positive reward (fructose) in an appetitive associative learning assay.
Mutant flies have reduced reactivity to electric shock compared to controls.
Mutant flies show normal learning (measured as 2 minute memory) in an olfactory aversive conditioning assay. However, middle-term memory (measured 3 hours after one cycle of training) is completely abolished in the mutant flies. Mutant flies have no long-term memory (measured 24 hours after 5 cycles of spaced training). Mutant flies are unable to form anesthesia-resistant memory (measured 24 hours after 5 cycles of massed training).
CASKβ-null mutants exhibit a significant reduction of acquired tolerance to ethanol vapor-induced sedation, and a significant increase in ethanol sensitivity, as compared to controls.
Mature CASKP18 males show a significantly lower overall level of immature-male courtship than the control. Courtship initiation latency of this mutant is also significantly longer than the control.
Mature CASKP18 males have a normal ability to sense and habituate to immature male pheromones.
CASKP18 homozygous flies exhibit significantly less movement than control flies, while CASKP18/+ flies fall somewhere in the middle of these two groups. Flies bearing CASKP18 in trans with CASKX-313, CASKX-307 or Df(3R)Exel6187 show similar activity levels to CASKP18 homozygotes.
The CASKP18 locomotor phenotype, whether as a homozygote or hemizygote, manifests as a complex deficit involving problems with motor initiation, motor maintenance, speed and acceleration. CASKP18/+ heterozygous flies show an intermediate phenotype, between control and homozygous phenotype, in all locomotor aspects examined.
Expression of CASKScer\UAS.cBa using Scer\GAL4C164 not only rescues all locomotor parameters that are deficient in CASKP18 flies, but enhances locomotor activity above wild type levels.
CASKP18 flies have normal circadian locomotor rhythms and wild type free running periods.
CASKβ-null has abnormal memory phenotype, suppressible by Scer\GAL4c305a/Hsap\CASKUAS.cMa
Expression of Hsap\CASKScer\UAS.cMa under the control of Scer\GAL4c305a fully rescues the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in CASKβ-null flies in an olfactory aversive conditioning assay.
CASKβ-null is rescued by CASKUAS.cBa/Scer\GAL4c305a
CASKβ-null is rescued by CASKUAS.cBa/Scer\GAL4C164
CASKβ-null is partially rescued by Scer\GAL4elav-C155/CASKUAS.cBa
CASKβ-null is partially rescued by Scer\GAL4elav-C155/CASKUAS.cBa
CASKβ-null is not rescued by Scer\GAL4VGlut1-OK371/CASKUAS.cBa
Expression of CASKScer\UAS.cBa under the control of Scer\GAL4c305a fully rescues the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in CASKβ-null flies in an olfactory aversive conditioning assay.
Expression of CASKScer\UAS.cBa using Scer\GAL4elav-C155 partially rescues all locomotor parameters that are deficient in CASKP18 flies.
Expression of CASKScer\UAS.cBa using Scer\GAL4C164 fully rescues all locomotor parameters that are deficient in CASKP18 flies.
Expression of CASKScer\UAS.cBa using Scer\GAL4VGlut-OK371 fails to rescues the locomotor parameters that are deficient in CASKP18 flies.
