Open Close
General Information
Symbol
Hsap\HTT16Q.FL.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0249416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt drives expression of the entire 3144 amino acid Hsap\HTT protein with a wild type polyQ tract of 16 amino acids.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\HTT16Q.FL.Scer\UAS under the control of Scer\GAL4elav.PU has no significant effect on nighttime sleep. A small, but significant increase in daytime sleep bout number is seen, but overall daytime rest and average daytime sleep bout length are normal.

Expression of Hsap\HD16Q.FL.Scer\UAS using the strong Scer\GAL4GMR.PF driver does not result in depigmentation or disorganization of ommatidia.

Flies expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4C164 do not show impaired motor performance as a function of age, as measured by a flying assay.

Aged flies expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4C164 do not show a neurodegenerative phenotype: motor neurons innervating the indirect flight muscles and neuromuscular junctions are normal.

Excitatory junction potentials (EJPs) at neuromuscular junctions of third instar larvae expressing Hsap\HD16Q.FL.Scer\UAS using Scer\GAL4elav-C155 are similar to controls at 1.2 mM, 0.25 mM and 0.6 mM extracellular Ca[2+].

Resting synaptic Ca[2+] levels at presynaptic terminals are elevated by ~2-fold in Hsap\HD16Q.FL.Scer\UAS, Scer\GAL4elav-C155 larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the htt98E2 homozygous mutant background induces prominent collapse of the thorax due to severe loss of the muscles below, these phenotypes are not observed in either the Hsap\MAPTScer\UAS.T:Avic\GFP expressing flies or the htt98E2 mutants alone and can be rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.

The age-dependent loss of climbing ability as well as the reduced lifespan characteristic for htt98E2 homozygous adults is partially suppressed by expression of Hsap\HTT16Q.FL.Scer\UAS under the Scer\GAL4arm.PS driver and further worsened by expression of Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 in the mutant background. This worsened phenotype can in turn also be fully partially rescued by co-expression of Hsap\HTT16Q.FL.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\HD16Q.FL.Scer\UAS
Hsap\HTT16Q.FL.Scer\UAS
Hsap\HTT16Q.FL.UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)