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General Information
Symbol
Scer\GAL4Gr64e.8
Species
S. cerevisiae
Name
FlyBase ID
FBal0249508
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gr64e-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 8kb Gr64e promoter fragment is fused upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4Gr64e.8 drives expression in about 12 sugar-sensing chemosensory neurons in the prothoracic leg. A subset of the leg neurons project to the ventral nerve cord then ascend through the cervical connective to the posterior gnathal gangion.

Scer\GAL4Gr64e.8 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in multidendritic neurons with ladder-like processes along the dorsal abdominal wall.

Scer\GAL4Gr64e.8 is expressed in the sugar-responsive neuron of almost all of the short, intermediate and long taste sensilla of the labellum.

Scer\GAL4Gr64e.8 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

Scer\GAL4Gr64e.8 drives expression in the gustatory receptor neurons of a subset of the labellar taste pegs and bristles, the labral sense organ and taste bristles in the leg. The gustatory receptor neurons projections in the brain are also labelled with Scer\GAL4Gr64e.8. These projections label the labial and pharyngeal nerves, and cervical connective and their entry point in the subesophageal ganglion. The projections of the gustatory receptor neurons labelled with Scer\GAL4Gr64e.8 are located close to the projections of sugar-sensitive neurons.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr64e.8 cells in adults significantly reduces preference for sucrose, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with orange light) of Scer\GAL4Gr64e.8 neurons in the absence of food prior to testing in adults does not affect food intake, compared to controls.

Inhibition (using Ork1Δ-C.UAS.EGFP) of Scer\GAL4Gr64e.8 cells in adults given a choice between sucrose solution and water does not affect water or sucrose consumption, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4Gr64e.8
Name Synonyms
Secondary FlyBase IDs
    References (14)