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General Information
Symbol
Scer\GAL4Gr64f.9.7
Species
S. cerevisiae
Name
FlyBase ID
FBal0249509
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gr64f-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 9.7kb Gr64f promoter fragment is fused upstream of sequence encoding a GAL4 driver.

Approximately 10kb of Gr64f promoter sequence is fused upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in the sugar-responsive neuron of all of the short, intermediate and long taste sensilla of the labellum.

Scer\GAL4Gr64f.9.7 drives expression in sweet taste receptor neurons in the labellar taste hairs, and in about 12 sugar-sensing chemosensory neurons in the prothoracic leg. A subset of the leg neurons project to the ventral nerve cord then ascend through the cervical connective to the posterior gnathal gangion; these neurons, unlike the other Scer\GAL4Gr64f.9.7-expression neurons, do not coexpress Scer\GAL4Gr5a.8.5 or Ecol\lexAVP16.Gr5a.cGa

All labellar and many of the tarsal S cells expressing Scer\GAL4Gr64f.9.7 send projections to the adult subesophageal zone.

Scer\GAL4Gr64f.9.7 is strongly expressed in the sugar-responsive neuron of all of the short, intermediate and long taste sensilla of the labellum.

Scer\GAL4Gr64f.9.7 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of Scer\GAL4Gr64f.9.7 cells in adult females results in a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls.

Inhibition (using Ctet\tetXTNT-LC.UAS) of Scer\GAL4Gr64f.9.7 cells in adults results in a significant reduction in proboscis extensions in response to sucrose or to fatty acids, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of the combined set of Scer\GAL4Gr33a-GAL4 and Scer\GAL4Gr64f.9.7 cells in adult females does not affect preference for oviposition on acid-containing medium, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 635 nm (red) light) of Scer\GAL4Gr64f.9.7 neurons results in a significant increase in proboscis extension reflex (PER) whether this is in the absense of nutritional stimuli or in the presence of 100 mM sucrose, compared to controls.

Inhibition (using Ctet\tetXTNT-LC.UAS) of Scer\GAL4Gr64f.9.7 cells in starved adult males significantly reduces preference for softer sucrose-containing agarose, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 633 nm (red) light) of Scer\GAL4Gr64f.9.7 cells in starved adult males significantly increases preference for softer sucrose-containing agarose, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr64f.9.7 cells in adults significantly reduces preference for sucrose, compared to controls. When inhibition is restricted to a smaller set of cells (using Ecol\lexAVP16.Gr5a.cGa and Scer\GAL80IVS.WPRE.8xlexAop) there is still a significant reduction in preference for sucrose, compared to controls; there is also a significant reduction in proboscis extension reflex probability in response to tarsal, but not labellar, sucrose, and a significant reduction in short term appetitive (sucrose), but not aversive, olfactory memory, compared to controls; significant sugar-induced suppression of angular and linear velocity is seen, similar to controls.

Inhibition (using Ctet\tetXUAS.cUa) of Scer\GAL4Gr64f.9.7 cells, in combination with a Gal80[ts] transgene to restrict expression to the few days of the adult stage prior to testing, significantly reduces the preference of starved flies for higher concentrations of sucrose, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with orange light) of Scer\GAL4Gr64f.9.7 neurons in the absence of food prior to testing in adults does not affect food intake, compared to controls.

Ablation of Scer\GAL4Gr64f.9.7-expressing neurons does not affect sensitivity to the gustatory sex pheromone (S,Z,Z)-CH503: as in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

Inhibition (using Ork1Δ-C.UAS.EGFP) of Scer\GAL4Gr64f.9.7 cells in adults given a choice between sucrose solution and water results in significantly reduced water, but not sucrose, consumption, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4Gr64f.9.7
Name Synonyms
Secondary FlyBase IDs
    References (45)