FlyBase Allele Report: Scer\GAL4[Gr64f.9.7]

ScerGAL4^Gr64f.9.7 drives expression in sweet taste receptor neurons in the labellar taste hairs, and in about 12 sugar-sensing chemosensory neurons in the prothoracic leg. A subset of the leg neurons project to the ventral nerve cord then ascend through the cervical connective to the posterior gnathal gangion; these neurons, unlike the other ScerGAL4^Gr64f.9.7-expression neurons, do not coexpress ScerGAL4^Gr5a.8.5 or EcollexA^{VP16.Gr5a.cGa}

(Thoma et al., 2016)

All labellar and many of the tarsal S cells expressing ScerGAL4^Gr64f.9.7 send projections to the adult subesophageal zone.

(Fujii et al., 2015)

ScerGAL4^Gr64f.9.7 is strongly expressed in the sugar-responsive neuron of all of the short, intermediate and long taste sensilla of the labellum.

(Weiss et al., 2011)

ScerGAL4^Gr64f.9.7 is expressed in all the sugar-sensitive receptor neurons of the gustatory sensilla of the labellum.

(Weiss et al., 2011)

Marker for

prothoracic leg taste bristle chemosensory neuron

(Ahn et al., 2017)

sugar-sensing neuron

(Yang et al., 2021, Zhang et al., 2020)

sugar-sensing neuron of taste bristle

(Ganguly et al., 2017, Fujii et al., 2015)

Reflects expression of

Gr64f

(Yang et al., 2021, Zhang et al., 2020, Ahn et al., 2017, Thoma et al., 2016, Fujii et al., 2015, Ling et al., 2014, Fan et al., 2013, Weiss et al., 2011, Weiss et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with alka¹, alka^P276A.UAS

(Mi et al., 2023)

abnormal chemosensory behavior | adult stage, with alka¹, alka^UAS.cMa

(Mi et al., 2023)

abnormal chemosensory behavior | adult stage, with Ctet\tetX^{TNT-LC.UAS.cUa}

(Zhan et al., 2016)

abnormal chemosensory behavior | adult stage, with Ctet\tetX^TNT-LC.UAS

(Ahn et al., 2017, Sánchez-Alcañiz et al., 2017)

abnormal chemosensory behavior | adult stage, with Ecol\lexA^{VP16.Gr5a.cGa}, Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Thoma et al., 2016)

abnormal chemosensory behavior | adult stage, with Ir56d^GD2096

(Ahn et al., 2017)

abnormal chemosensory behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Sánchez-Alcañiz et al., 2017)

abnormal feeding behavior | adult stage, with Ctet\tetX^{TNT-LC.UAS.cUa}

(Zhan et al., 2016)

abnormal feeding behavior | adult stage, with Ork1^{Δ-C.UAS.EGFP}

(Sun et al., 2014)

abnormal locomotor behavior | adult stage, with Ecol\lexA^{VP16.Gr5a.cGa}, Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal locomotor behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Thoma et al., 2016)

abnormal memory | adult stage, with Ecol\lexA^{VP16.Gr5a.cGa}, Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

abnormal neurophysiology | adult stage, with alka¹, alka^P276A.UAS

(Mi et al., 2023)

abnormal neurophysiology | adult stage, with Cbeta\DT^A-I.UAS

(Dweck et al., 2022)

abnormal oviposition | chemical conditional, with Hsap\KCNJ2^{IVS.10xUAS.EGFP}

(Wu et al., 2019)

abnormal proboscis extension reflex, with alka¹, alka^UAS.cMa

(Mi et al., 2023)

abnormal proboscis extension reflex, with Ctet\tetX^TNT-LC.UAS

(Ahn et al., 2017)

abnormal proboscis extension reflex | adult stage, with Ctet\tetX^TNT-LC.UAS

(Li and Montell, 2021)

abnormal proboscis extension reflex | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Klapoetke et al., 2014)

abnormal proboscis extension reflex | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Kim et al., 2017)

abnormal tarsal response, with Ecol\lexA^{VP16.Gr5a.cGa}, Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^{IVS.WPRE.8xlexAop}

(Thoma et al., 2016)

Phenotype Manifest In

large labellar taste bristle, with Cbeta\DT^A-I.UAS

(Dweck et al., 2022)

L-type sensillum L7, with alka¹, alka^P276A.UAS

(Mi et al., 2023)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of Scer\GAL4^Gr64f.9.7 cells in adult females results in a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls.

(Wu et al., 2019)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Gr64f.9.7 cells in adults results in a significant reduction in proboscis extensions in response to sucrose or to fatty acids, compared to controls.

(Ahn et al., 2017)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of the combined set of Scer\GAL4^Gr33a-GAL4 and Scer\GAL4^Gr64f.9.7 cells in adult females does not affect preference for oviposition on acid-containing medium, compared to controls.

(Chen and Amrein, 2017)

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 635 nm (red) light) of Scer\GAL4^Gr64f.9.7 neurons results in a significant increase in proboscis extension reflex (PER) whether this is in the absense of nutritional stimuli or in the presence of 100 mM sucrose, compared to controls.

(Kim et al., 2017)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Gr64f.9.7 cells in starved adult males significantly reduces preference for softer sucrose-containing agarose, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 633 nm (red) light) of Scer\GAL4^Gr64f.9.7 cells in starved adult males significantly increases preference for softer sucrose-containing agarose, compared to controls.

(Sánchez-Alcañiz et al., 2017)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Gr64f.9.7 cells in adults significantly reduces preference for sucrose, compared to controls. When inhibition is restricted to a smaller set of cells (using Ecol\lexA^{VP16.Gr5a.cGa} and Scer\GAL80^{IVS.WPRE.8xlexAop}) there is still a significant reduction in preference for sucrose, compared to controls; there is also a significant reduction in proboscis extension reflex probability in response to tarsal, but not labellar, sucrose, and a significant reduction in short term appetitive (sucrose), but not aversive, olfactory memory, compared to controls; significant sugar-induced suppression of angular and linear velocity is seen, similar to controls.

(Thoma et al., 2016)

Inhibition (using Ctet\tetX^UAS.cUa) of Scer\GAL4^Gr64f.9.7 cells, in combination with a Gal80[ts] transgene to restrict expression to the few days of the adult stage prior to testing, significantly reduces the preference of starved flies for higher concentrations of sucrose, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with orange light) of Scer\GAL4^Gr64f.9.7 neurons in the absence of food prior to testing in adults does not affect food intake, compared to controls.

(Zhan et al., 2016)

Ablation of Scer\GAL4^Gr64f.9.7-expressing neurons does not affect sensitivity to the gustatory sex pheromone (S,Z,Z)-CH503: as in wild type, courtship defects are seen in these flies in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Inhibition (using Ork1^{Δ-C.UAS.EGFP}) of Scer\GAL4^Gr64f.9.7 cells in adults given a choice between sucrose solution and water results in significantly reduced water, but not sucrose, consumption, compared to controls.

(Sun et al., 2014)

External Data

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Gr22e^UAS.cSa, Gr32a^UAS.cSa, Gr66a^UAS.Tag:MYC, Scer\GAL4^Gr64f.9.7 has abnormal taste perception | adult stage phenotype, suppressible | partially by Df(3L)ΔGr64af

(Sung et al., 2017)

Gr22e^UAS.cSa, Gr32a^UAS.cSa, Gr66a^UAS.Tag:MYC, Scer\GAL4^Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Df(3L)ΔGr64af