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General Information
Symbol
Scer\GAL4GMR18H11
Species
S. cerevisiae
Name
FlyBase ID
FBal0250346
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R18H11-GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 3354 base pair fragment from the flanking non-coding or intronic region of pdfr is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4GMR18H11 drives expression in a subset of DN1 neurons in the adult dorsal brain; this subset overlaps the DN1 neuron subset labeled by antibodies to VGlut.

Scer\GAL4GMR18H11 drives expression in four to six Dh31-positive DN1-neurons.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and 627 nm (red) light) of cells expressing both Scer\GAL4GMR18H11 and Ecol\lexAGMR67F03, in adults significantly increases sleep, compared to controls.

Optogenetic inhibition (using Gthe\ACR1UAS.d.EYFP with 540 nm (green) light) of Scer\GAL4GMR18H11 cells significantly reduces sleep, compared to controls.

Activation of Scer\GAL4GMR18H11-expressing neurons (using heat activation of a TrpA1 construct) significantly suppresses sleep, with the effect being strongest in the hours before and after dawn. Since this effect can be suppressed by inhibiting Scer\GAL4 expression in clock neurons (using Scer\GAL80) and Scer\GAL4GMR18H11 is normally active in a restricted subset of four to six Dh31-expressing DN1 clock neurons, this indicates that Dh31-expressing DN1 circadian clock neurons are intrinsically wake promoting.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Scer\GAL4GMR18H11
Name Synonyms
Secondary FlyBase IDs
    References (13)