FlyBase Allele Report: Scer\GAL4[GMR18H11]

General Information

Symbol

Scer\GAL4^GMR18H11

Species

S. cerevisiae

Name

FlyBase ID

FBal0250346

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR18H11-GAL4}

Also Known As

R18H11-GAL4, R18H11

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR18H11-GAL4}

Cytology

Description

A 3354 base pair fragment from the flanking non-coding or intronic region of pdfr is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR18H11

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Drives expression primarily in a subset of DN1p neurons that also express Dh31.

(FlyBase Curators, 2017)

ScerGAL4^GMR18H11 drives expression in a subset of DN1 neurons in the adult dorsal brain; this subset overlaps the DN1 neuron subset labeled by antibodies to VGlut1.

(Guo et al., 2016)

ScerGAL4^GMR18H11 drives expression in four to six Dh31-positive DN1-neurons.

(Kunst et al., 2014)

Marker for

DN1 neuron

(Sun et al., 2022)

Reflects expression of

Pdfr

(Guo et al., 2016, Kunst et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus} with Scer\FLP1^L.8xlexAop and 627 nm (red) light) of cells expressing both Scer\GAL4^GMR18H11 and Ecol\lexA^GMR67F03, in adults significantly increases sleep, compared to controls.

Optogenetic inhibition (using Gthe\ACR1^UAS.d.EYFP with 540 nm (green) light) of Scer\GAL4^GMR18H11 cells significantly reduces sleep, compared to controls.

(Guo et al., 2018)

Activation of Scer\GAL4^GMR18H11-expressing neurons (using heat activation of a TrpA1 construct) significantly suppresses sleep, with the effect being strongest in the hours before and after dawn. Since this effect can be suppressed by inhibiting Scer\GAL4 expression in clock neurons (using Scer\GAL80) and Scer\GAL4^GMR18H11 is normally active in a restricted subset of four to six Dh31-expressing DN1 clock neurons, this indicates that Dh31-expressing DN1 circadian clock neurons are intrinsically wake promoting.

(Kunst et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pdf^{UAS.ML.Tag:SS(trypsin).Tag:MYC.Tag:GPI(lynx1)}, Scer\GAL4^GMR18H11 has abnormal sleep phenotype, suppressible | partially by Dh31^KG09001/Dh31^KG09001

(Kunst et al., 2014)

NOT Suppressor of