FlyBase Allele Report: Scer\GAL4[GMR23E10]

General Information

Symbol

Scer\GAL4^GMR23E10

Species

S. cerevisiae

Name

FlyBase ID

FBal0250760

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR23E10-GAL4}

Also Known As

R23E10-GAL4, 23E10-GAL4, R23E10Gal4, R23E10 Gal4, R23E10

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR23E10-GAL4}

Cytology

Description

A 771 base pair fragment from the flanking non-coding or intronic region of AlstR is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR23E10

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P7 -- pharate adult stage P15

adult lateral fan-shaped body tangential-protocerebrum neuron ExFl2

(Chakravarti Dilley et al., 2020)

Additional Information

Statement

Reference

Drives expression in a group of neurons that project to the dorsal layer of the fan-shaped body. These neurons receive inputs from ellipsoid body R2 neurons.

(Ni et al., 2019)

Drives expression in a group of neurons that project to the dorsal layer of the fan-shaped body.

(FlyBase Curators, 2017)

ScerGAL4^GMR23E10 drives expression exclusively in the dorsal part of the fan-shaped body.

(Donlea et al., 2014)

Marker for

Reflects expression of

AstA-R1

(Ni et al., 2019)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Bhal\NaChBac^UAS.cNa

(Troup et al., 2018)

abnormal locomotor behavior | adult stage, with Inx6^GD3692

(Troup et al., 2018)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Troup et al., 2018)

abnormal memory | adult stage, with cv-c^RNAi.UAS

(Donlea et al., 2014)

abnormal neurophysiology, with sand^GD1866

(Pimentel et al., 2016)

abnormal neurophysiology | adult stage, with Dop1R2^KK110947

(Pimentel et al., 2016)

abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Troup et al., 2018)

abnormal sleep, with Dop1R2^KK110947

(Pimentel et al., 2016)

abnormal sleep, with Hk^KK109058

(Pimentel et al., 2016)

abnormal sleep, with qvr^KK109915

(Pimentel et al., 2016)

abnormal sleep, with sand^GD1866

(Pimentel et al., 2016)

abnormal sleep, with Sh^KK109112

(Pimentel et al., 2016)

abnormal sleep, with Shab^KK111134

(Pimentel et al., 2016)

abnormal sleep | adult stage, with Bhal\NaChBac^UAS.cNa

(Troup et al., 2018)

abnormal sleep | adult stage, with cv-c^RNAi.UAS

(Donlea et al., 2014)

abnormal sleep | adult stage, with Inx6^GD3692

(Troup et al., 2018)

abnormal sleep | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Troup et al., 2018)

abnormal stress response | adult stage, with cv-c^RNAi.UAS

(Donlea et al., 2014)

increased sleep | adult stage, with TrpA1^UAS.cUa

(Gong et al., 2021)

Phenotype Manifest In

fan-shaped body, with Dop1R2^KK110947

(Pimentel et al., 2016)

fan-shaped body, with sand^GD1866

(Pimentel et al., 2016)

fan-shaped body | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Troup et al., 2018)

Detailed Description

Statement

Reference

Chronic activation (using Bhal\NaChBac^UAS.cNa) of Scer\GAL4^GMR23E10 cells in adults results in a more negative correlation between nighttime, or daytime, sleep duration and behavioral responsiveness (movement) to disturbance, compared to controls.

Prolonged optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults significantly increases sleep duration, significantly decreases responsiveness in sleeping and awake flies, and significantly increases sleep intensity, compared to controls, with no effect on walking speed in awake flies prior to disturbance stimulus, compared to controls; responsiveness is also reduced following acute activation, compared to controls.

Acute optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults results in increased membrane potential and secondary spikes in the activated cells and a significant increase in extracellular local field potential power in the dorsal fan-shaped body.

Transiently increasing or decreasing synaptic activity (using Syx1A^T254I.UAS or shi^1.UAS, respectively, at 31[o]C) of Scer\GAL4^GMR23E10 cells in adults does not significantly affect sleep or behavioral responsiveness, compared to controls.

(Troup et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR23E10, TrpA1^UAS.cUa has increased sleep | adult stage phenotype, suppressible by Iswi^{QUAS.HMS00628}/Ncra\QF^{elav-C155-QF2}

(Gong et al., 2021)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10 has abnormal sleep | adult stage | conditional phenotype, suppressible | partially by Inx6^GD3692, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Inx6^GD3692, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10 has abnormal neurophysiology | adult stage | conditional phenotype, suppressible | partially by Inx6^GD3692, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Inx6^GD3692, Scer\GAL4^GMR23E10 has abnormal sleep | adult stage phenotype, suppressible | conditional | partially by Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Suppressor of

Statement

Reference

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal sleep | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal locomotor behavior | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of abnormal neurophysiology | adult stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10 is a suppressor | conditional | partially of abnormal sleep | adult stage phenotype of Inx6^GD3692, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10 has fan-shaped body | conditional phenotype, suppressible | partially by Inx6^GD3692, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Suppressor of

Statement

Reference

Inx6^GD3692, Scer\GAL4^GMR23E10 is a suppressor | partially of fan-shaped body | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR23E10

(Troup et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Inx6^GD3692 under the control of Scer\GAL4^GMR23E10 blocks the secondary spikes and local field potential oscillations, but not the increased membrane potential, induced by acute optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults; this expression also suppresses the sleep duration and sleep intensity increases and the responsiveness decrease caused by prolonged optogenetic activation.

Prolonged optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with (red) light) of Scer\GAL4^GMR23E10 cells in adults suppresses the sleep duration decrease, but not the responsiveness increase or the sleep intensity decrease caused by expression of Inx6^GD3692 under the control of Scer\GAL4^GMR23E10.

(Troup et al., 2018)

Complementation and Rescue Data

Partially rescues

Scer\GAL4^GMR23E10/cv-c^UAS.cDa partially rescues cv-c^C524/cv-c^MB03717

(Donlea et al., 2014)

Fails to rescue

Scer\GAL4^GMR23E10/Dop2R^UAS.cDa fails to rescue Dop2R^attP

(Deng et al., 2019)

Comments

Images (0)

Mutant

Wild-type