A 4407 base pair fragment from the flanking non-coding or intronic region of hth is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.
A FLP/FRT intersectional strategy shows expression of Scer\GAL4GMR46F09 in ventrolateral protocerebrum projection neuron 1 when combined with Ecol\lexAfru.P1.
courtship behavior defective | male limited, with Ctet\tetXTNT-LC.FRT.stop.UAS, Ecol\lexAfru.P1, Scer\FLP1L.8xlexAop
courtship behavior defective | male limited | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.20xUAS.FRT.Venus, Ecol\lexAfru.P1, Scer\FLP1L.8xlexAop
Inhibition (using Ctet\TeTxLCFRT.stop.UAS with Scer\FLP1L.8xlexAop) of neurons expressing Ecol\lexAfru.P1 and Scer\GAL4GMR46F09 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.
Optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexAfru.P1 and Scer\GAL4GMR46F09 significantly increases male-male courtship chaining, compared to controls.