A 3718 base pair fragment from the flanking non-coding or intronic region of mspo is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.
A FLP/FRT intersectional strategy shows expression of Scer\GAL4GMR49F09 in antennal mechanosensory and motor center B1 projection neuron when combined with Ecol\lexAfru.P1.
abnormal courtship behavior | male limited, with Ctet\tetXTNT-LC.FRT.stop.UAS, Ecol\lexAfru.P1, Scer\FLP1L.8xlexAop
Inhibition (using Ctet\TeTxLCFRT.stop.UAS with Scer\FLP1L.8xlexAop) of neurons expressing Ecol\lexAfru.P1 and Scer\GAL4GMR49F09 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.
Optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexAfru.P1 and Scer\GAL4GMR49F09 does not affect male-male courtship chaining, compared to controls.