FlyBase Allele Report: Scer\GAL4[GMR58E02]

General Information

Symbol

Scer\GAL4^GMR58E02

Species

S. cerevisiae

Name

FlyBase ID

FBal0253714

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR58E02-GAL4}

Also Known As

R58E02-GAL4, R58E02, GMR58E02-GAL4, 58E02-GAL4, PAM-Gal4, R58E02GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR58E02-GAL4}

Cytology

Description

A 1235 base pair fragment from the flanking non-coding or intronic region of DAT is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR58E02

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron h1

(Saumweber et al., 2018, Rohwedder et al., 2016)

larval mushroom body dopaminergic neuron i1

(Saumweber et al., 2018, Rohwedder et al., 2016)

larval mushroom body dopaminergic neuron j1

(Saumweber et al., 2018, Rohwedder et al., 2016)

larval mushroom body dopaminergic neuron k1

(Rohwedder et al., 2016)

adult stage

mushroom body medial lobe | restricted

(Riemensperger et al., 2013)

adult dopaminergic PAM neuron

(Yamagata et al., 2015, Vogt et al., 2014, Perisse et al., 2013, Riemensperger et al., 2013, Liu et al., 2012)

adult brain | restricted

(Shuai et al., 2015, Yamagata et al., 2015)

mushroom body extrinsic neuron

(Shuai et al., 2015)

mushroom body gamma lobe slice 1

(Shuai et al., 2015)

mushroom body gamma lobe slice 2

(Shuai et al., 2015)

mushroom body gamma lobe slice 3

(Shuai et al., 2015)

mushroom body gamma lobe slice 4

(Shuai et al., 2015)

mushroom body gamma lobe slice 5

(Shuai et al., 2015)

mushroom body beta lobe slice 1

(Shuai et al., 2015)

mushroom body beta lobe slice 2

(Shuai et al., 2015)

mushroom body beta' lobe slice 1

(Shuai et al., 2015)

mushroom body beta' lobe slice 2

(Shuai et al., 2015)

mushroom body alpha lobe slice 1

(Shuai et al., 2015)

dopaminergic PAM neuron 13

(Shuai et al., 2015)

dopaminergic PAM neuron 14

(Shuai et al., 2015)

adult optic lobe glial cell

(Liu et al., 2012)

adult dopaminergic PAM neuron | subset

(Otto et al., 2020, Meissner et al., 2019, Huetteroth et al., 2015)

medial lobe of adult mushroom body

(Huetteroth et al., 2015)

Additional Information

Statement

Reference

Drives expression in a subset of dopaminergic protocerebral anterior medial (PAM) neurons.

(FlyBase Curators, 2017)

ScerGAL4^GMR58E02 drives expression in ple positive larval dopaminergic pPAM neurons. Expression in larval dopaminergic pPAM2 neuron is stochastic and very rare.

(Rohwedder et al., 2016)

ScerGAL4^GMR58E02 drives expression in mushroom body extrinsic neurons that innervate mushroom body gamma lobe slices 1, 2, 3, 4 and 5, mushroom body beta lobe slices 1 and 2, mushroom body beta' lobe slices 1 and 2 and mushroom body alpha lobe slice 1. Expression partially overlaps with that driven by EcollexA^GMR24E12 in 13 dopaminergic PAM neurons 13 and 14.

(Shuai et al., 2015)

ScerGAL4^GMR58E02 labels ~90 dopaminergic PAM neurons that innervate the medial lobe of the adult mushroom body.

(Huetteroth et al., 2015)

ScerGAL4^GMR58E02 expression found in around 80% of dopaminergic PAM neurons. Labeled neurons project to mushroom body medial lobe.

(Riemensperger et al., 2013)

ScerGAL4^GMR58E02 labels dopaminergic PAM neurons that innervate the alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells.

(Perisse et al., 2013)

ScerGAL4^GMR58E02 drives expression in DAT positive dopaminergic PAM neurons as well as adult optic lobe glial cells.

(Liu et al., 2012)

Marker for

adult dopaminergic PAM neuron

(Otto et al., 2020, Vogt et al., 2014)

Reflects expression of

DAT

(Liu et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal adult locomotory behavior | progressive, with Spyo\Cas9^UAS.uM, mbt^HD_CFD00807

(Pütz et al., 2021)

abnormal learning | temperature conditional, with shi^1.UAS

(Lin et al., 2014)

abnormal learning | temperature conditional, with shi^{ts1.IVS.p10.20xUAS}

(Lin et al., 2014)

abnormal locomotor behavior | adult stage, with Hsap\SNCA^A30P.UAS

(Riemensperger et al., 2013)

abnormal locomotor behavior | adult stage, with Lam^UAS.GFP

(Lin et al., 2022)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^ple.PF, TrpA1^UAS.(B).cKa

(Sun et al., 2018)

abnormal locomotor behavior | adult stage | heat sensitive, with shi^1.UAS

(Sun et al., 2018)

abnormal memory | adult stage | :heat sensitive | conditional, with shi^1.UAS

(Vogt et al., 2014)

abnormal memory | adult stage | conditional, with Crei\ChR2^UAS.cSa

(Rohwedder et al., 2016)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Felsenberg et al., 2018, Rohwedder et al., 2016, Huetteroth et al., 2015, Liu et al., 2012)

abnormal memory | adult stage | heat sensitive, with shi^{ts1.IVS.p10.20xUAS}

(Yamagata et al., 2015)

abnormal memory | adult stage | heat sensitive, with TrpA1^UAS.(B).cKa

(Huetteroth et al., 2015, Yamagata et al., 2015, Vogt et al., 2014, Liu et al., 2012)

abnormal memory | larval stage, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Eichler et al., 2017)

abnormal neuroanatomy, with Fer2^RNAi.UAS.4

(Bou Dib et al., 2014)

abnormal neuroanatomy, with Fer2^RNAi.UAS.5

(Bou Dib et al., 2014)

abnormal neuroanatomy | adult stage | progressive, with Lrrk^I1915T.UAS

(Miozzo et al., 2022)

abnormal neuroanatomy | adult stage | progressive, with Lrrk^UAS.cIa

(Miozzo et al., 2022)

decreased cell number | adult stage, with foxo^GD4348

(Tas et al., 2018)

decreased cell number | adult stage, with foxo^HMS00422

(Tas et al., 2018)

decreased cell number | adult stage, with foxo^HMS00793

(Tas et al., 2018)

decreased cell number | adult stage | progressive, with Lrrk^I1915T.UAS

(Miozzo et al., 2022)

decreased cell number | adult stage | progressive, with Lrrk^UAS.cIa

(Miozzo et al., 2022)

decreased efficacy of long-term memory | adult stage, with Gthe\ACR1^UAS.d.EYFP

(Jacob and Waddell, 2020)

decreased efficacy of long-term memory | adult stage, with shi^1.UAS

(Jacob and Waddell, 2020)

decreased rate of adult locomotory behavior, with Aprt^KK108921

(Petitgas et al., 2024)

decreased rate of adult locomotory behavior, with Aprt^KK108921, Scer\GAL4^ple.PF

(Petitgas et al., 2024)

short lived, with Spyo\Cas9^UAS.uM, mbt^HD_CFD00807

(Pütz et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 30-33[o]C) of Scer\GAL4^GMR58E02 neurons in adults, only during aversive (CS+) odor re-exposure following training, but not only during testing, prevents the aversive olfactory memory extinction that results from re-exposure, compared to controls.

(Felsenberg et al., 2018)

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^GMR58E02 cells in adults results in a small, but significant, increase in startle-induced climbing behavior, compared to controls; inhibition of the combined set of Scer\GAL4^GMR58E02 and Scer\GAL4^ple.PF cells in adults does not affect startle-induced climbing behavior, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^GMR58E02 cells in adults does not affect startle-induced climbing behavior, compared to controls; thermogenetic activation of the combined set of Scer\GAL4^GMR58E02 and Scer\GAL4^ple.PF cells in adults significantly reduces startle-induced climbing behavior, compared to controls.

(Sun et al., 2018)

Acute optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of Scer\GAL4^GMR58E02 neurons in first instar larvae results in a significant short-term preference for a paired odor, compared to controls.

(Eichler et al., 2017)

Ablation (using hid^UAS.cUa and rpr^UAS.cUa) of Scer\GAL4^GMR58E02 neurons significantly impairs short-term appetitive olfactory memory following training with fructose or sorbitol, but not arabinose, compared to controls; there is no effect on aversive olfactory memory following training with quinine, compared to controls.

Inhibition (using shi^1.UAS at 35[o]C) of Scer\GAL4^GMR58E02 neurons significantly impairs short-term appetitive olfactory memory following training with fructose, compared to controls.

Optogenetic activation (using Crei\ChR2^UAS.cSa with 470 nm (blue) light) of Scer\GAL4^GMR58E02 neurons, in combination with a paired odor, results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

(Rohwedder et al., 2016)

Inhibition (using shi^ts.UAS.cUa at 33[o]C) of Scer\GAL4^GMR58E02 cells in adults does not affect short-term associative aversive olfactory memory, compared to controls.

(Yamagata et al., 2016)

Blocking the output (using shi^1.UAS at 33[o]C) of Scer\GAL4^GMR58E02 neurons shortly before and during training significantly impairs long-term olfactory memory in starved flies trained with sucrose, compared to controls.

Acute thermogenetic activation (using TrpA1^UAS.(B).cKa at 33[o]C) of Scer\GAL4^GMR58E02 neurons results in a significant long-term preference for a paired odor (artificial memory) in starved flies, compared to controls.

(Huetteroth et al., 2015)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^GMR58E02 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, does not affect 3 hour aversive olfactory memory, compared to controls.

(Shuai et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^GMR58E02 cells in adults, in combination with a paired odor, results in a significant increase in short-term and long-term associative attractive olfactory memory, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 33[o]C) of Scer\GAL4^GMR58E02 cells in adults during training significantly reduces short-term and long-term associative appetitive (sucrose) olfactory memory, compared to controls.

(Yamagata et al., 2015)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^GMR58E02 cells in adults significantly reduces appetitive, but not aversive, 1-min visual memory, compared to controls; inhibition does not significantly affect sugar preference, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of Scer\GAL4^GMR58E02 cells, in combination with a paired light stimulus, results in a significant increase in short-term associative attractive visual memory, compared to controls.

(Vogt et al., 2014)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^GMR58E02 cells in starved adults, in combination with a paired odor, results in a significant increase in 2-min associative attractive olfactory memory, compared to controls.

Inhibition (using shi^1.UAS at 32[o]C) of Scer\GAL4^GMR58E02 cells before and during training, but not after training and during testing, significantly reduces 30-min associative appetitive olfactory memory, compared to controls; inhibition has no effect on naive sugar preference, compared to controls.

(Liu et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR58E02, TrpA1^UAS.(B).cKa has abnormal memory | adult stage | heat sensitive phenotype, non-enhanceable by Tbh^nM18

(Liu et al., 2012)

Suppressed by