Site-directed optogenetic activation (using Crei\ChR2^Scer\UAS.cSa with blue light) of Scer\GAL4^GMR81E10 neurons induces significant grooming behavior compared to control flies; light directed to the posterior dorsal body surface elicits wing grooming whereas illumination of the anterior dorsal surface elicits notum grooming.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light) of Scer\GAL4^GMR81E10 neurons across the body induces significant grooming behavior; flies groom their heads at the onset of a five-second red light stimulus and then transition to groom their posterior bodies during the period after the light is turned off. Even when these flies are engaged in posterior grooming, delivery of the next red light stimulus causes flies to switch to head grooming. In decapitated flies, red-light activation of Scer\GAL4^GMR81E10 neurons directly elicits immediate posterior grooming behavior.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^GMR81E10 cells in fed adult females significantly increases spontaneous proboscis extensions, compared to controls, with no effect on the intake of water or other solutions.

Inactivation (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^GMR81E10 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, in adult females does not significantly affect consumption of sugar solution following starvation, compared to controls.