Allele Dmel\rhea^{R367A.Ubi-p63E}

General Information
Symbol	Dmel\rhea^{R367A.Ubi-p63E}	Species	D. melanogaster
Name		FlyBase ID	FBal0256724
Feature type	allele	Associated gene	Dmel\rhea

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Tanentzapf and Brown, 2006) in vitro construct - regulatory fusion (Tanentzapf and Brown, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Ubi-p63E promoter sequences drive expression of a fragment of rhea (starting 457 bases upstream of the ATG to 100 bases downstream of the stop codon), carrying the mutation R367A. (Tanentzapf and Brown, 2006)
Carried in construct	P{Ubi-p63E-talin.R367A} (Tanentzapf and Brown, 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	muscle attachment site \| embryonic stage \| germline clone, with Df(3L)79a (Tanentzapf and Brown, 2006) presumptive embryonic/larval muscle system \| embryonic stage \| germline clone, with Df(3L)79a (Tanentzapf and Brown, 2006)
Detailed Description
	Statement Reference Animals expressing rhea[R367A.Ubi-p63E] in a Df(3L)79a mutant background display a late-muscle-detachment phenotype. (Tanentzapf and Brown, 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Df(3L)79a, rhea^{R367A.Ubi-p63E} has muscle attachment site \| germline clone \| embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/if^{ΔGFFNR.Scer\UAS}/Scer\GAL4^how-24B (Tanentzapf and Brown, 2006) Df(3L)79a, rhea^{R367A.Ubi-p63E} has presumptive embryonic/larval muscle system \| germline clone \| embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/if^{ΔGFFNR.Scer\UAS}/Scer\GAL4^how-24B (Tanentzapf and Brown, 2006)
Additional Comments
Genetic Interactions
Statement Reference Expression of if[ΔGFFNR.Scer\UAS] under the control of Scer\GAL4[how-24B] rescues the late-muscle-detachment phenotype of embryos expressing rhea[R367A.Ubi-p63E] in a Df(3L)79a mutant background. (Tanentzapf and Brown, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	rhea^{R367A.Ubi-p63E} partially rescues Df(3L)79a (Tanentzapf and Brown, 2006)
Comments	Expression of rhea[R367A.Ubi-p63E] fails to rescue the lethality of of Df(3L)79a germ-line clones. However, expression does rescue defects in gut migration, germ-band retraction, dorsal closure, and muscle attachment. Late-onset muscle detachment is observed in these animals. Retraction of one end of some of the ventral longitudinal muscles from the attachment site is observed. (Tanentzapf and Brown, 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	rhea^{R367A.Ubi-p63E}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Ellis et al., 2011, J. Cell Sci. 124(11): 1844--1856 In vivo functional analysis reveals specific roles for the integrin-binding sites of talin. [FBrf0213701] Tanentzapf and Brown, 2006, Nat. Cell Biol. 8(6): 601--606 An interaction between integrin and the talin FERM domain mediates integrin activation but not linkage to the cytoskeleton. [FBrf0193338]

Allele Dmel\rheaR367A.Ubi-p63E

Allele Dmel\rhea^{R367A.Ubi-p63E}