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General Information
Symbol
Dmel\Gr61a1
Species
D. melanogaster
Name
FlyBase ID
FBal0256895
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔGr61a, Δ61a
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of the Gr61a gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Gr61a1 mutants exhibit a statistically significant reduction in the response to trehalose, melezitose, m-glucoside, and glucose. However, responses to sucrose, maltose, maltotriose, fructose and glycerol are unaffected.

Gr61a1 Gr5aLEXA Df(3L)ΔGr64 mutants expressing P{t15.3.R1} and P{UAS-Rescue.R2} (i.e. lacking all 8 sweet gustatory receptor genes) do not exhibit any defects in fructose preference in two-choice preference assays.

Electrophysiological recordings of the L-type labellar sensilla of mutant flies in response to a number of sugars are very similar to those of control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Adult females homozygous for Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64, or with Gr5aLEXA/+, Gr61a1/+ and Gr64fLEXA/Df(3L)ΔGr64, have a significant increase in preference for softer 100 mM sucrose-containing, but not plain, agarose for egg laying, compared to controls; these mutants also have a significant reduction in preference for 100 mM sucrose-containing (rather than plain) agarose for egg laying, compared to controls.

Adults with homozygous Gr5aLEXA, Gr61a1 and Df(3L)ΔGr64 have proboscis extension rates that are significantly reduced in response to sucrose and significantly increased in response to some fatty acids, compared to controls.

Gr5aLEXA, Gr61a1, Df(3L)ΔGr64 triple homozygotes that also bear P{t15.3.R1} (P{t15.3.R1} rescues the lethality of Df(3L)ΔGr64 homozygosity) have impaired responsiveness to sweet chemical: sweet taste 5b sensilla GRNs virtually lack the expected responsiveness to the chemicals Sucrose, Fructose, Maltose, Trehalose, Glucose, Melezitose, Arabinose and Glycerol, as compared to controls; sweet taste 5v sensilla GRNs show the expected responsive only to Sucrose and Fructose, but lack responses to Maltose, Trehalose and Glucose.

Expressing Gr64aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5v sensilla GRNs only to Maltose but does not affect the responses of 5b sensilla GRNs.

Expressing Gr61aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a moderate response of 5v sensilla GRNs only to Glucose but does not affect the responses of 5b sensilla GRNs.

Expressing Gr5aUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores a small response of 5v sensilla GRNs only to Trehalose but does not affect the responses of 5b sensilla GRNs.

Co-expressing Gr64aUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Maltose and Sucrose, but does not affect the responses by 5v sensilla GRNs.

Co-expressing Gr64bUAS.cUa and Gr64eUAS.cUa under the control of Scer\GAL4Gr61a.4.9 restores the response of 5b sensilla GRNs only to Glycerol, but does not affect the responses by 5v sensilla GRNs.

The Ca[2+] response of taste neurons in the forelegs to fructose, sucrose, glucose, trehalose, arabinose or maltose is abolished in flies triply homozygous Gr43aGAL4 ; Gr61a1 Df(3L)ΔGr64.

Gr61a1 Df(3L)ΔGr64 double mutant flies show a significantly reduced proboscis extension reflex to fructose and sucrose and fail to respond to glucose.

Gr43aGAL4 ; Gr61a1 Df(3L)ΔGr64 triple mutant flies show completely lack a proboscis extension reflex to fructose and sucrose.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (9)