Allele Dmel\cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0256921
Feature type	allele	Associated gene	Dmel\cnn

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Zhang and Megraw, 2007) in vitro construct - deletion (Zhang and Megraw, 2007) in vitro construct - regulatory fusion (Zhang and Megraw, 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of a cnn coding sequence which lacks the amino terminal 17 amino acids of the maternal cnn-RA transcript and in which 13 core amino acid residues in the conserved "motif 1" have been deleted. The cnn sequences are tagged with T:Avic\GFP[EGFP]. (Zhang and Megraw, 2007)
Carried in construct	P{UASp-cnn.Δ1} (Zhang and Megraw, 2007)
Tagged with	T:Avic\GFP^EGFP (Zhang and Megraw, 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| maternal effect, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Zhang and Megraw, 2007)
Phenotype Manifest In
	centriolar satellite, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Zhang and Megraw, 2007) centrosome, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Zhang and Megraw, 2007)
Detailed Description
	Statement Reference Embryos expressing cnn[Δ1.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] do not hatch. Centrosome duplication appears normal in these embryos, but centrosome separation fails. The mutant embryos also have reduced numbers of centrosomal satellites. Expression of cnn[Δ1.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[tub.PU] from late embryogenesis onwards is not zygotically lethal. (Zhang and Megraw, 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue cnn^HK21/cnn^25cn1 (Zhang and Megraw, 2007)
Comments	Expression of cnn[Δ1.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] fails to rescue the maternal effect lethality of cnn[HK21]/cnn[25cn1] embryos. (Zhang and Megraw, 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Zhang and Megraw, 2007, Mol. Biol. Cell 18(10): 4037--4049 Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1. [FBrf0202160]

Allele Dmel\cnnΔ1.Scer\UAS.T:Avic\GFP-EGFP

Allele Dmel\cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP}