FB2025_01 , released February 20, 2025
Allele: Dmel\Crtc25-3
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General Information
Symbol
Dmel\Crtc25-3
Species
D. melanogaster
Name
FlyBase ID
FBal0256962
Feature type
allele
Associated gene
Dmel\Crtc
Associated Insertion(s)
Carried in Construct
Also Known As
TORC25-3
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Wang et al., 2008)
Progenitor genotype
CrtcEY00004
(Wang et al., 2008)
Cytology
Description

Δ2-3-induced transposition of P{EPgy2}TORCEY00004 generates an approximate 10kb deletion removing the entire TORC coding sequence.

(Wang et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In response to water-only starvation, TORC25-3 flies live an average of less than 36 hours, while wild-type flies live for an average 72 hours.

      TORC25-3 flies are sensitive to oxidative stress. Following exposure to paraquat, their mean survival time is reduced by 50% relative to controls.

      TORC25-3 flies exhibit a similar food intake to wild-type flies and comparable or elevated levels of physical activity, particularly during fasting.

      Respiratory quotients are indistinguishable between TORC25-3 mutant and wild-type flies, indicating that glucose oxidation is appropriately induced during feeding, transitioning to fat burning in response to starvation. Consistent with their starvation sensitivity, however, TORC25-3 mutant flies have lower amounts of stored glycogen and lipid relative to wild-type. These reductions appear specific for adults, as wild-type and TORC25-3 mutant larvae have comparable lipid levels.

      (Wang et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Crtc25-3 has short lived phenotype, non-enhanceable by foxo25/foxo[+]

      (Wang et al., 2008)

      Crtc25-3 has abnormal stress response phenotype, non-enhanceable by foxo25/foxo[+]

      (Wang et al., 2008)
      NOT suppressed by
      Statement
      Reference

      Crtc25-3 has short lived phenotype, non-suppressible by foxo25/foxo[+]

      (Wang et al., 2008)

      Crtc25-3 has abnormal stress response phenotype, non-suppressible by foxo25/foxo[+]

      (Wang et al., 2008)
      Suppressor of
      Statement
      Reference

      Crtc25-3 is a suppressor of long lived | calorie restriction conditional phenotype of Sik2Δ41

      (Choi et al., 2011)
      Other
      Statement
      Reference

      Crtc25-3, foxo21, foxo25 has lethal phenotype

      (Wang et al., 2008)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Crtc25-3 is a suppressor of eye phenotype of Pp2B-14Dact.UAS, Scer\GAL4GMR.PF

      (Wang et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A TORC25-3 heterozygous background decreases the lipid and glycogen levels in a fed and starved SIK2Δ41 mutant. A TORC25-3 homozygous background further decreases the lipid and glycogen levels in a fed and starved SIK2Δ41 mutant. As the level of TORC decreases, the flies become more sensitive to starvation.

      (Choi et al., 2011)

      A foxo25 heterozygous background has no effect on survival or lipid accumulation in starved TORC25-3 homozygotes.

      Removal of both copies of foxo (foxo25/foxo21) is lethal in TORC25-3 homozygotes.

      Eye-specific expression of Pp2B-14Dact.Scer\UAS under the control of Scer\GAL4GMR.PF fails to promote a rough eye phenotype in TORC25-3 mutant flies.

      (Wang et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Crtc25-3 is rescued by CrtcUAS.cWa/Scer\GAL4AD.elav

      (Wang et al., 2008)
      Not rescued by

      Crtc25-3 is not rescued by CrtcUAS.cWa/Scer\GAL4r4

      (Wang et al., 2008)
      Comments

      Pan-neuronal expression of TORCScer\UAS.cWa under the control of Scer\GAL4AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2 rescues both the starvation- and paraquat-sensitive phenotypes of TORC25-3 mutant flies. In keeping with this, total lipid levels are elevated in rescued flies. However, lipid levels are only partially restored.

      TORCScer\UAS.cWa expression in the fat body under the control of Scer\GAL4r4 does not rescue the starvation sensitivity seen in TORC25-3 mutant flies.

      (Wang et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CRTC25-3
      (Dondi et al., 2024, Choi et al., 2015, Choi et al., 2011)
      Crtc25-3
      (Shen et al., 2016)
      TORC25-3
      (Yin et al., 2022, Wang et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)