Allele Dmel\bft^Δ263a

General Information
Symbol	Dmel\bft^Δ263a	Species	D. melanogaster
Name		FlyBase ID	FBal0257039
Feature type	allele	Associated gene	Dmel\bft

Allele class
Mutagen	recombination, ends-out gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Hilgers et al., 2010) recombination (Hilgers et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Ends-out homologous recombination has resulted in a 350bp deletion that removes the mir-263a miRNA hairpin (which is encoded by bft). The miRNA hairpin has been replaced by a mini-w marker. (Hilgers et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	visible (Hilgers et al., 2010) visible (with bft²⁴) (Hilgers et al., 2010) visible (with bft^Δ263a-G4) (Hilgers et al., 2010)
Phenotype Manifest In
	interommatidial bristle (Hilgers et al., 2010) interommatidial bristle (with bft²⁴) (Hilgers et al., 2010) interommatidial bristle (with bft^Δ263a-G4) (Hilgers et al., 2010)
Detailed Description
	Statement Reference Homozygous and bft[Δ263a]/bft[24] flies show loss of approximately 80% of interommatidial bristles. bft[Δ263a]/bft[Δ263a-G4] flies show loss of interommatidial bristles. The mutant retina appears normal in homozygous flies at 24 hours after puparium formation (APF). However, the majority of bristle shaft progenitor cells are missing in the mutant retina at 40 hours APF. Apoptotic nuclei corresponding to the bristle shaft cells are seen in the mutant retina at 35 hours APF. (Hilgers et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference bft²⁴/bft^Δ263a has visible phenotype, enhanceable by Df(3L)X-21.2/mir-263b^Δ (Hilgers et al., 2010)
Suppressed by
Statement Reference bft²⁴/bft^Δ263a has visible phenotype, suppressible by Scer\GAL4^{mir-263b-Δ-G4}/mir-263b^{Scer\UAS.T:Disc\RFP-DsRed2} (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has visible phenotype, suppressible \| partially by W[+]/W¹ (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has visible phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/W[+] (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has visible phenotype, suppressible by W^GD1673/Scer\GAL4^{mir-263b-Δ-G4} (Hilgers et al., 2010)
Enhancer of
Statement Reference bft^Δ263a is an enhancer of visible phenotype of W^Ala5.GMR (Hilgers et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference bft²⁴/bft^Δ263a has interommatidial bristle phenotype, enhanceable by Df(3L)X-21.2/mir-263b^Δ (Hilgers et al., 2010)
Suppressed by
Statement Reference bft²⁴/bft^Δ263a has interommatidial bristle phenotype, suppressible by Scer\GAL4^{mir-263b-Δ-G4}/mir-263b^{Scer\UAS.T:Disc\RFP-DsRed2} (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has interommatidial bristle phenotype, suppressible \| partially by W[+]/W¹ (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has interommatidial bristle phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/W[+] (Hilgers et al., 2010) bft^unspecified/bft^Δ263a has interommatidial bristle phenotype, suppressible by W^GD1673/Scer\GAL4^{mir-263b-Δ-G4} (Hilgers et al., 2010)
Enhancer of
Statement Reference bft^Δ263a is an enhancer of eye phenotype of W^Ala5.GMR (Hilgers et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference The loss of interommatidial bristles seen in bft[Δ263a]/bft[24] flies is significantly enhanced if the flies are also carrying mir-263b[Δ]/Df(3L)X-21.2. Expression of mir-263b[Scer\UAS.T:Disc\RFP-DsRed2] under the control of Scer\GAL4[mir-263b-Δ-G4] rescues the loss of interommatidial bristles which is seen in bft[Δ263a]/bft[24] flies. W[05014]/+ and W[1]/+ each partially suppress the loss of interommatidial bristles which is seen in bft[Δ263a]/bft[unspecified] flies. Expression of W[GD1673] under the control of Scer\GAL4[mir-263b-Δ-G4] strongly suppresses the loss of interommatidial bristles which is seen in bft[Δ263a]/bft[unspecified] flies. (Hilgers et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	bft²⁴/bft^Δ263a is partially rescued by bft^{Scer\UAS.T:Disc\RFP-DsRed2} (Hilgers et al., 2010)
Comments	Leaky expression of bft[Scer\UAS.T:Disc\RFP-DsRed2] in the absence of a Scer\GAL4 driver partially rescues the loss of interommatidial bristles which is seen in bft[Δ263a]/bft[24] flies. (Hilgers et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	bft^Δ263a Δ263a (Hilgers et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Hilgers et al., 2010, PLoS Biol. 8(6): e1000396 Drosophila microRNAs 263a/b Confer Robustness during Development by Protecting Nascent Sense Organs from Apoptosis. [FBrf0211074]

Allele Dmel\bftΔ263a

Allele Dmel\bft^Δ263a