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General Information
Symbol
Dmel\mir-bftΔ263a
Species
D. melanogaster
Name
FlyBase ID
FBal0257039
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

350bp deletion that removes the mir-263a miRNA hairpin (which is encoded by bft). The miRNA hairpin has been replaced by a mini-w marker.

Insertion components
TI{TI}mir-bftΔ263a
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

bftΔ263a homozygous adults present an impaired immune response, as shown by the increased load of internal commensal bacteria and by the decreased survival upon bacterial infection with Pseudomonas aeruginosa, as compared to controls; the adult posterior midgut of these animals are significantly shorter and wider, frequently exhibit a multi-layered epithelium and evidence of anoikis of enterocytes, as shown by delamination and the presence of large lysosomes, and exhibit significant increases in the number of intestinal stem cells, which undergo symmetric cell divisions, in the number of mitotic cells at 14 days (but not at 7 days) post eclosion, in the transverse cell area (but not in the transverse nuclear area) and cell volume of enterocytes, as compared to controls.

bftΔ263a homozygous mutant clones within a mosaic adult midgut present significant increases in the numbers of cells and intestinal stem cells, and also induce a cell non-autonomous increase in the numbers of mitotic cells and intestinal stem cells, as compared to control clones.

The adult posterior midguts of bftΔ263a/bft24 transheterozygotes display a significant increase in the number of mitotic cells at 14 days post eclosion, as compared to controls.

bftΔ263a/bftΔ263a-G4 transheterozygotes exhibit an overall increase in the gut pH and a significant increase in the number of mitotic cells in the adult posterior midgut at 14 days post eclosion, as compared to controls; these adult posterior midguts also exhibit a significant decrease in the peritrophic matrix thickness, which becomes significantly thicker upon the additional expression of bftScer\UAS.T:Ppyr\LUC under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 allele, where it replaces the bft hairpin sequence, as compared to controls.

Homozygous and bftΔ263a/Df(2L)BSC323 females show a significant decrease in median lifespan compared to controls.

Homozygous and bftΔ263a/bft24 flies show loss of approximately 80% of interommatidial bristles.

bftΔ263a/bftΔ263a-G4 flies show loss of interommatidial bristles.

The mutant retina appears normal in homozygous flies at 24 hours after puparium formation (APF). However, the majority of bristle shaft progenitor cells are missing in the mutant retina at 40 hours APF. Apoptotic nuclei corresponding to the bristle shaft cells are seen in the mutant retina at 35 hours APF.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

mir-bftΔ263a is an enhancer of eye phenotype of hidAla5.GMR

Other
Additional Comments
Genetic Interactions
Statement
Reference

The expression of either NachJF02566 or NachGD2039 suppresses the increased number of mitotic cells in the adult posterior midgut exhibited by bftΔ263a/bftΔ263a-G4 transheterozygotes (expression under the control of Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 alele, where it replaces the bft hairpin sequence) or bftΔ263a homozygotes (expression under the control of Scer\GAL4Myo31DF-NP0001); the expression of NachJF02566 under the control of Scer\GAL4bft-Δ263a-G4 also suppresses the overall increase in the gut pH and the decreased thickness of the adult posterior midgut peritrophic matrix of bftΔ263a/bftΔ263a-G4 heterozygotes, even leading to its significant increase in thickness as compared to wild-type controls.

The increased number of mitotic cells observed in the adult posterior midgut of bftΔ263a homozygotes is suppressed by the expression of either ppk6JF01919, ppk16JF01931 or ppk28JF02153, under the control of Scer\GAL4Myo31DF-NP0001.

The loss of interommatidial bristles seen in bftΔ263a/bft24 flies is significantly enhanced if the flies are also carrying mir-263bΔ/Df(3L)X-21.2.

Expression of mir-263bScer\UAS.T:Disc\RFP-DsRed2 under the control of Scer\GAL4mir-263b-Δ-G4 rescues the loss of interommatidial bristles which is seen in bftΔ263a/bft24 flies.

W05014/+ and W1/+ each partially suppress the loss of interommatidial bristles which is seen in bftΔ263a/bftunspecified flies.

Expression of WGD1673 under the control of Scer\GAL4mir-263b-Δ-G4 strongly suppresses the loss of interommatidial bristles which is seen in bftΔ263a/bftunspecified flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4bft-Δ263a-G4, the inherent Gal4 driver in the bftΔ263a-G4 allele, where it replaces the bft hairpin sequence, suppresses the overall increase in the gut pH, and suppresses both the increased number of mitotic cells and the decreased thickness of the peritrophic matrix in the posterior midgut of bftΔ263a/bftΔ263a-G4 transheterozygous mutant adults, even leading to a significant increase in the peritrophic matrix thickness as compared to wild-type controls.

The expression of bftScer\UAS.T:Ppyr\LUC driven by Scer\GAL4Myo31DF-NP0001 does not significantly affect (it does not rescue or enhance) the increased number of mitotic cells observed in the adult posterior midgut of bftΔ263a homozygotes.

Leaky expression of bftScer\UAS.T:Disc\RFP-DsRed2 in the absence of a Scer\GAL4 driver partially rescues the loss of interommatidial bristles which is seen in bftΔ263a/bft24 flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
bftΔ263a
miR-263aΔ
mir-263aKO
mir-bftΔ263a
Name Synonyms
Secondary FlyBase IDs
    References (5)