FlyBase Allele Report: Dmel\Akt[HMS00007]

General Information

Symbol

Dmel\Akt^HMS00007

Species

D. melanogaster

Name

FlyBase ID

FBal0257056

Feature type

allele

Associated gene

Dmel\Akt

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00007}

Also Known As

UAS-AKT^RNAi, UAS-Akt RNAi, UAS-Akt1 RNAi, HMS00007, UAS-Akt-RNAi, Akt1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00007}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00007

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Lee et al., 2021)

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut epithelium | somatic clone, with Scer\GAL4^Tub.PU

(Andriatsilavo et al., 2018)

eye, with Scer\GAL4^eya.enh1+E+2

(Pletcher et al., 2019)

female germline stem cell, with Scer\GAL4^nanos.PG

(Wang et al., 2019)

germarium, with Scer\GAL4^nanos.PG

(Wang et al., 2019)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Andriatsilavo et al., 2018)

ovary, with Scer\GAL4^{VP16.nanos.UTR}

(Wei and Lilly, 2014)

wing disc | third instar larval stage, with Scer\GAL4^en-e16E

(Sun et al., 2020)

Detailed Description

Statement

Reference

Expression of Akt^HMS00007 under the control of Scer\GAL4^en-e16E (in combination with Gal80[ts] to control the timing of expression) leads to increased cell death in third instar larval wing discs.

(Sun et al., 2020)

Expression under the control of Scer\GAL4^eya.enh1+E+2 results in a small eye phenotype.

(Pletcher et al., 2019)

The expression of Akt1^HMS00007 under the control of Scer\GAL4^nos.PG leads to germline degeneration in the germarium.

(Wang et al., 2019)

Adulthood-generated midgut clones expressing Akt1^HMS00007 under the control of Scer\GAL4^Tub.PU have significantly fewer cells, but an insignificant change in the proportion of intestinal stem cells, as compared to control clones.

(Andriatsilavo et al., 2018)

Females heterozygous for Akt1^HMS00007 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to Akt1^HMS00007 males results in: no eggs; egg/embryonic phenotype: no vitellogenic egg chambers; oogenesis defect.

(Sopko et al., 2014)

Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of Akt1^HMS00007 results in a block to oogenesis with females containing extremely small ovaries.

(Wei and Lilly, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Akt^HMS00007, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Suppressor of

Statement

Reference

Akt^HMS00007/Scer\GAL4^Tub.PU is a suppressor | partially of increased cell number | somatic clone | adult stage phenotype of spen⁵

(Andriatsilavo et al., 2018)

Akt^HMS00007, Scer\GAL4^NP0001 is a suppressor of increased cell size phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Akt^HMS00007, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Akt^HMS00007, Scer\GAL4^ppk.PG is a suppressor of abnormal neuroanatomy | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Akt^HMS00007, Scer\GAL4^{VP16.nanos.UTR} has ovary phenotype, suppressible by Tsc1^GL00012, Scer\GAL4^{VP16.nanos.UTR}

(Wei and Lilly, 2014)

NOT suppressed by

Statement

Reference

Akt^HMS00007, Scer\GAL4^{VP16.nanos.UTR} has ovary phenotype, non-suppressible by Nprl2^RNAi.UAS.V22, Scer\GAL4^{VP16.nanos.UTR}

(Wei and Lilly, 2014)

Akt^HMS00007, Scer\GAL4^{VP16.nanos.UTR} has ovary phenotype, non-suppressible by Nprl3^RNAi.UAS.V22, Scer\GAL4^{VP16.nanos.UTR}

(Wei and Lilly, 2014)

Enhancer of

Statement

Reference

Akt^HMS00007, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Suppressor of

Statement

Reference

Akt^HMS00007/Scer\GAL4^Tub.PU is a suppressor | partially of adult midgut epithelium | somatic clone phenotype of spen⁵

(Andriatsilavo et al., 2018)

Akt^HMS00007/Scer\GAL4^Tub.PU is a suppressor | partially of intestinal stem cell | somatic clone | adult stage phenotype of spen⁵

(Andriatsilavo et al., 2018)

Akt^HMS00007, Scer\GAL4^NP0001 is a suppressor of adult midgut epithelium phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Akt^HMS00007, Scer\GAL4^insc-Mz1407 is a suppressor of type II neuroblast | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Akt^HMS00007, Scer\GAL4^ppk.PG is a suppressor of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Cul1^GD9650, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Akt1^HMS00007 suppresses the increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

Co-expression of Tsc1^GL00012, but not Nprl2^{dsRNA.shRNA.Scer\UAS.P\T} or Nprl3^{dsRNA.shRNA.Scer\UAS.P\T}, rescues the small ovary phenotype resulting from Scer\GAL4^{nos.UTR.T:Hsim\VP16}-mediated expression of Akt1^HMS00007.

(Wei and Lilly, 2014)

Co-expression of Akt1^HMS00007 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Lee et al., 2013)

Expression of Akt1^HMS00007 dramatically suppresses the dendrite pruning defects seen in ddaC neurons expressing lin19^GD9650 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

(Wong et al., 2013)

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2B^Intron5.UAS under the control of Scer\GAL4^GMR.PU is strongly enhanced by co-expression of Akt1^HMS00007.

(West et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33615

y¹ v¹; P{TRiP.HMS00007}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Akt1^HMS00007

Akt^HMS00007

Akt^RNAi

(Wen et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (30)

Report Sections

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