FlyBase Allele Report: Dmel\dpp[HMS00011]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\dpp^HMS00011

General Information

Symbol

Dmel\dpp^HMS00011

Species

D. melanogaster

Name

FlyBase ID

FBal0257059

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00011}

Also Known As

UAS-dpp^RNAi, UAS-dpp RNAi, UAS-dppRNAi, dpp^RNAi

Key Links

Genomic Maps

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00011}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2013)

abnormal circadian rhythm | heat sensitive, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS

(Beckwith et al., 2013)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mhc.PW

(Robles-Murguia et al., 2020)

abnormal neuroanatomy | larval stage, with Scer\GAL4^GMR82E12

(Kaneko et al., 2024)

abnormal proboscis extension reflex, with Scer\GAL4^Mhc.PW

(Robles-Murguia et al., 2020)

abnormal proboscis extension reflex | RU486 conditional, with Scer\GAL4^GS.Act88F

(Robles-Murguia et al., 2020)

abnormal size | adult stage | cell autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

abnormal size | adult stage | cell non-autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

decreased cell number | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

short lived | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Bai et al., 2013)

Phenotype Manifest In

axon terminus | larval stage, with Scer\GAL4^GMR82E12

(Kaneko et al., 2024)

cystoblast, with Scer\GAL4^C587

(Liu et al., 2015)

dense core granule | adult stage, with Scer\GAL4^NP0261

(Redhai et al., 2016)

germarium, with Scer\GAL4^C587

(Liu et al., 2015)

larval nociceptive neuropil, with Scer\GAL4^GMR82E12

(Kaneko et al., 2024)

lysosome | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^NP0261

(Redhai et al., 2016)

male accessory gland secondary cell | cell autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

male accessory gland secondary cell | cell non-autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

multivesicular body | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

nucleus | adult stage | cell autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

nucleus | adult stage | cell non-autonomous, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

secretory vesicle | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

Detailed Description

Statement

Reference

Expression of dpp^HMS00011 under the control of Scer\GAL4^Mhc.PW leads to an increase in the spontaneous locomotion and in the probability of proboscis extension reflex (also occurs with Scer\GAL4^GS.Act88F and RU486 feeding) when compared to control adults.

(Robles-Murguia et al., 2020)

The adulthood-only expression of dpp^HMS00011 under the control of Scer\GAL4^esg.PU (in combination with Gal80[ts], for temporal regulation of expression) leads to significant decreases in the relative size of secondary cell nuclei, as compared to the neighboring main cell nuclei; this effect is both cell-autonomous and non-cell autonomous. The Scer\GAL4^esg.PU-driven expression also leads to a significant decrease in the number of non-acidic secretory vesicles, but not of acidic secretory vesicles, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^dsx.KI and Gal80[ts] leads to a small but significant increase the size of the largest multivesicular body/lysosome and leads to a small but significant increase in the number of non-acidic, but not of acidic, secretory vesicles within accessory gland secondary cells, as compared to controls; this is associated with a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.

The adulthood-only expression of dpp^HMS00011 under the combined control of Scer\GAL4^spi-NP0261 and Gal80[ts] results in both virgin and mated males showing a significant increase in the number of dense core granule compartments within accessory gland secondary cells, as compared to matched controls.

(Redhai et al., 2016)

Germaria in flies expressing dpp^HMS00011 under the control of Scer\GAL4^C587 exhibit fewer cystoblasts, but no significant difference in germline stem cell number, as compared to controls.

(Liu et al., 2015)

The expression of dpp^HMS00011 under the control of Scer\GAL4^C587 leads to some germaria losing germ line stem cells, as compared to controls.

(Ma et al., 2014)

Scer\GAL4^da.Switch.PT dpp^HMS00011 adults, fed with RU486 to activate expression, show reduced lifespan compared to controls.

(Bai et al., 2013)

Down-regulation of dpp in Pdf-positive neurons, through expression of dpp^HMS00011 under the control of Scer\GAL4^P2.4.Pdf induces strong locomotor arrhythmicity. Expression of dpp^HMS00011 under the control of Scer\GAL4^tim.PE and suppressed in Pdf-positive cells through the presence of Scer\GAL80^Pdf.PS results in some rescue of the arrhythmic phenotype, with arrhythmicity found in approximately 75-80% of flies.

(Beckwith et al., 2013)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

dpp^HMS00011/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^C587/dpp^HMS00011 is a suppressor | partially of increased cell number | oogenesis phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

dpp^HMS00011/Scer\GAL4^Bx-MS1096 is an enhancer of wing hinge phenotype of Scer\GAL4^Bx-MS1096, hng1^KK103642

(Shukla et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^C587/dpp^HMS00011 is a suppressor | partially of germarium phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Scer\GAL4^C587/dpp^HMS00011 is a suppressor | partially of female germline stem cell phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expressing dpp^HMS00011 enhances the wing hinge phenotype seen when hng1^KK103642 is expressed under the control of Scer\GAL4^Bx-MS1096.

(Shukla et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

33618

y¹ v¹; P{TRiP.HMS00011}attP2

HMS00011

y¹ v¹; P{TRiP.HMS00011}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^BL33618

(Liu et al., 2015)

dpp^HMS00011

dpp^RNAi33

(Velarde et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections