FlyBase Allele Report: Dmel\Iswi[HMS00628]

General Information

Symbol

Dmel\Iswi^HMS00628

Species

D. melanogaster

Name

FlyBase ID

FBal0257313

Feature type

allele

Associated gene

Dmel\Iswi

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00628}

Also Known As

UAS-shIswi, HMS00628

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Ni et al., 2010.12.1)

in vitro construct - regulatory fusion

(Ni et al., 2010.12.1)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{TRiP.HMS00628}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Iswi

Also carries

loxP

recombinase target site

Associated Sequence Features

dsRNA-HMS00628

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

(Börner et al., 2016)

increased cell death, with Scer\GAL4^tj.PU

(Börner et al., 2016)

lethal, with Scer\GAL4^Act5C.PI

(Dietz et al., 2015)

size defective, with Scer\GAL4^tj.PU

(Börner et al., 2016)

size defective | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

size defective | adult stage, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

(Börner et al., 2016)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

egg, with Scer\GAL4^tj.PU

(Börner et al., 2016)

egg chamber, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

(Börner et al., 2016)

egg chamber, with Scer\GAL4^tj.PU

(Börner et al., 2016)

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

follicle cell, with Scer\GAL4^tj.PU

(Börner et al., 2016)

germarium, with Scer\GAL4^tj.PU

(Börner et al., 2016)

ovary, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

(Börner et al., 2016)

wing blade, with Scer\GAL4^en.PU

(Dietz et al., 2015)

Detailed Description

Statement

Reference

Expression of Iswi^HMS00628 under the control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in an agametic phenotype with small ovaries and defects in early oogenesis. If expression is restricted to adulthood by rearing flies at 18[o]C until adulthood and subsequently incubating adult females at 29[o]C for three days, the egg chambers display increased apoptosis and cell packaging defects, as compared with controls.

Expression of Iswi^HMS00628 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} or Scer\GAL4^C587 does not result in any apparent phenotype, as compared with controls.

Expression of Iswi^HMS00628 under the control of Scer\GAL4^tj.PU results in increased apoptosis in the egg chamber; cell packaging defects in the egg chamber including compound egg chambers, egg chambers with 3 or more oocytes in non-opposing positions, or egg chambers with abnormal cell numbers ranging from only one to eight cells with a decreased number of ring canals, observable in the germarium; gaps in the follicle cell epithelium; additional stalk-like structures; and yields a "dumpless" phenotype with short eggs, as compared with controls.

(Börner et al., 2016)

Expression of Iswi^HMS00628 results in: small rough eye phenotype (with Scer\GAL4^ey.PH), occasional lack of anterior crossvein (with Scer\GAL4^nub.PK), wing notches and occasional blisters at 25[o]C (with Scer\GAL4^en.PU) - no phenotype at 18[o]C. Scer\GAL4^Act5C.PI-driven expression is lethal; no overt adult phenotype is observed with the Scer\GAL4^GMR.PF driver.

(Dietz et al., 2015)

Expression of Iswi^HMS00628 under the control of Scer\GAL4^bbg-C96 does not result in any obvious wing phenotype.

(Ellis et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^en.PU has visible | adult stage phenotype, suppressible | partially by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^{dsRNA.Sym.UAS}

(Ellis et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^en.PU has wing blade phenotype, suppressible | partially by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^bbg-C96 is an enhancer of wing phenotype of Scer\GAL4^bbg-C96, dom^{dsRNA.Sym.UAS}

(Ellis et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotype observed in adult flies expressing Iswi^HMS00628 under the control of Scer\GAL4^en.PU is weakly suppressed by co-expression of htt^GD14943.

(Dietz et al., 2015)

Co-expression of Iswi^HMS00628 enhances the wing nicking phenotype of flies expressing dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96.

(Ellis et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32845

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00628}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Iswi^HMS00628

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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