FlyBase Allele Report: Dmel\Iswi[HMS00628]

Adults expressing Iswi^HMS00628 under the control of Scer\GAL4^elav.PU display decreased climbing ability; they present a small adult brain and structural mushroom body defects: smaller vertical and horizontal lobes; bilaterally missing vertical lobe.

(Li et al., 2021)

Expression of Iswi^HMS00628 under the control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in an agametic phenotype with small ovaries and defects in early oogenesis. If expression is restricted to adulthood by rearing flies at 18[o]C until adulthood and subsequently incubating adult females at 29[o]C for three days, the egg chambers display increased apoptosis and cell packaging defects, as compared with controls.

Expression of Iswi^HMS00628 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} or Scer\GAL4^C587 does not result in any apparent phenotype, as compared with controls.

Expression of Iswi^HMS00628 under the control of Scer\GAL4^tj.PU results in increased apoptosis in the egg chamber; cell packaging defects in the egg chamber including compound egg chambers, egg chambers with 3 or more oocytes in non-opposing positions, or egg chambers with abnormal cell numbers ranging from only one to eight cells with a decreased number of ring canals, observable in the germarium; gaps in the follicle cell epithelium; additional stalk-like structures; and yields a "dumpless" phenotype with short eggs, as compared with controls.

(Börner et al., 2016)

Expression of Iswi^HMS00628 results in: small rough eye phenotype (with Scer\GAL4^ey.PH), occasional lack of anterior crossvein (with Scer\GAL4^nub.PK), wing notches and occasional blisters at 25[o]C (with Scer\GAL4^en.PU) - no phenotype at 18[o]C. Scer\GAL4^Act5C.PI-driven expression is lethal; no overt adult phenotype is observed with the Scer\GAL4^GMR.PF driver.

(Dietz et al., 2015)

Expression of Iswi^HMS00628 under the control of Scer\GAL4^bbg-C96 does not result in any obvious wing phenotype.

(Ellis et al., 2015)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, suppressible by Hsap\SMARCA1^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased efficacy of memory | adult stage phenotype, suppressible by Hsap\SMARCA1^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased sleep | adult stage phenotype, suppressible by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased sleep | adult stage phenotype, suppressible | partially by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021, Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased size | adult stage phenotype, suppressible | partially by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage phenotype, suppressible by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^en.PU has visible | adult stage phenotype, suppressible | partially by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

NOT suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased sleep | adult stage phenotype, non-suppressible by Hsap\SMARCA1^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased efficacy of memory | adult stage phenotype, non-suppressible by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\SMARCA5^R592Q.UAS, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased size | adult stage phenotype, non-suppressible by Hsap\SMARCA5^R592Q.UAS, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has decreased size | adult stage phenotype, non-suppressible by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\SMARCA5^R592Q.UAS, Scer\GAL4^elav.PU

(Li et al., 2021)

Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Ellis et al., 2015)

NOT Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-enhancer of increased cell death | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-enhancer of increased cell death | larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^HMS01490

(Sharp et al., 2021)

Suppressor of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^Switch1.106 is a suppressor of long lived | female phenotype of Scer\GAL4^Switch1.106, foxo^UAS.cFa

(Martínez Corrales et al., 2022)

Iswi^HMS00628, Scer\GAL4^Switch1.106 is a suppressor of abnormal adult locomotory behavior phenotype of Scer\GAL4^Switch1.106, foxo^UAS.cFa

(Martínez Corrales et al., 2022)

Iswi^HMS00628, Scer\GAL4^Switch1.106 is a suppressor of chemical resistant phenotype of Scer\GAL4^Switch1.106, foxo^UAS.cFa

(Martínez Corrales et al., 2022)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a suppressor of increased size | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^hh-Gal4 is a suppressor of increased size | larval stage phenotype of Scer\GAL4^hh-Gal4, dlg1^HMS01954

(Sharp et al., 2021)

NOT Suppressor of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of increased size | larval stage phenotype of Scer\GAL4^ptc-559.1, yki^S168A.UAS

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of increased size | larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^HMS01490

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of increased cell death | larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^HMS01490

(Sharp et al., 2021)

Other

Statement

Reference

Hsap\SMARCA5^R592Q.UAS, Iswi^HMS00628, Scer\GAL4^elav.PU has short lived phenotype

(Gong et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^elav.PU has lobe system of adult mushroom body phenotype, suppressible by Hsap\SMARCA1^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has lobe system of adult mushroom body phenotype, suppressible | partially by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Gong et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has mushroom body vertical lobe | adult stage phenotype, suppressible | partially by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^en.PU has wing blade phenotype, suppressible | partially by htt^GD14943, Scer\GAL4^en.PU

(Dietz et al., 2015)

NOT suppressed by

Statement

Reference

Iswi^HMS00628, Scer\GAL4^elav.PU has medial lobe of adult mushroom body phenotype, non-suppressible by Hsap\SMARCA5^UAS.cGa, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has medial lobe of adult mushroom body phenotype, non-suppressible by Hsap\SMARCA5^R592Q.UAS, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has medial lobe of adult mushroom body phenotype, non-suppressible by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has mushroom body vertical lobe | adult stage phenotype, non-suppressible by Hsap\SMARCA5^R592Q.UAS, Scer\GAL4^elav.PU

(Li et al., 2021)

Iswi^HMS00628, Scer\GAL4^elav.PU has mushroom body vertical lobe | adult stage phenotype, non-suppressible by Hsap\SMARCA5^{del268-319.UAS}, Scer\GAL4^elav.PU

(Li et al., 2021)

Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^bbg-C96 is an enhancer of wing phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Ellis et al., 2015)

NOT Enhancer of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-enhancer of wing disc | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-enhancer of wing disc | larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^HMS01490

(Sharp et al., 2021)

Suppressor of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a suppressor of wing hinge primordium | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^hh-Gal4 is a suppressor | partially of wing disc | larval stage phenotype of Scer\GAL4^hh-Gal4, dlg1^HMS01954

(Sharp et al., 2021)

NOT Suppressor of

Statement

Reference

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of wing disc | larval stage phenotype of Scer\GAL4^ptc-559.1, dlg1^GD4689

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of wing disc | larval stage phenotype of Scer\GAL4^ptc-559.1, yki^S168A.UAS

(Sharp et al., 2021)

Iswi^HMS00628, Scer\GAL4^ptc-559.1 is a non-suppressor of wing disc | larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^HMS01490

(Sharp et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotype observed in adult flies expressing Iswi^HMS00628 under the control of Scer\GAL4^en.PU is weakly suppressed by co-expression of htt^GD14943.

(Dietz et al., 2015)

Co-expression of Iswi^HMS00628 enhances the wing nicking phenotype of flies expressing dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96.

(Ellis et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Iswi^HMS00628 is rescued by Scer\GAL4^elav.PU/Iswi^{UAS.RR.Tag:FLAG,Tag:HA}

(Gong et al., 2021)

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Mutant

Wild-type