FlyBase Allele Report: Dmel\sima[HMS00833]

General Information

Symbol

Dmel\sima^HMS00833

Species

D. melanogaster

Name

FlyBase ID

FBal0257513

Feature type

allele

Associated gene

Dmel\sima

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00833}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00833}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00833

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Leigh disease

modeled by Surf1^RNAi.UAS

(Cagin et al., 2015)

ameliorates Parkinson's disease

modeled by park²⁵

(Cagin et al., 2015)

ameliorates acute myeloid leukemia

modeled by Hsap\KRAS^G12V.UAS

(Zhu et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^da.PU

(Cagin et al., 2015)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Cagin et al., 2015)

Phenotype Manifest In

mitochondrion | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Cagin et al., 2015)

neuromuscular junction | third instar larval stage, with Scer\GAL4^VGlut1-OK371

(Cagin et al., 2015)

Detailed Description

Statement

Reference

Expression of sima^HMS00833 under the control of Scer\GAL4^Toll-6-D42 does not significantly affect adult climbing ability or survival nor does it induce wing inflation defects. The number as well as volume of mitochondria at neuromuscular junctions in third instar larvae is reduced upon Scer\GAL4^VGlut-OK371-driven expression of sima^HMS00833 but the number of active zones is unaffected.

Expression of sima^HMS00833 has no significant affect on the climbing ability of adult flies when controlled by Scer\GAL4^nSyb.PU but the locomotion is strongly reduced when driven by Scer\GAL4^da.PU.

(Cagin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^da.PU, sima^HMS00833 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by park²⁵

(Cagin et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PU/sima^HMS00833 is a suppressor | partially of abnormal locomotor behavior | male | adult stage phenotype of Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS

(Cagin et al., 2015)

Scer\GAL4^nSyb.PU/sima^HMS00833 is a suppressor of visible | adult stage phenotype of Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS

(Cagin et al., 2015)

Scer\GAL4^da.PU/sima^HMS00833 is a suppressor of abnormal locomotor behavior | adult stage phenotype of park²⁵

(Cagin et al., 2015)

sima^HMS00833/Scer\GAL4^Toll-6-D42 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^Toll-6-D42, TFAM^UAS.cCa

(Cagin et al., 2015)

sima^HMS00833/Scer\GAL4^Toll-6-D42 is a suppressor of visible | adult stage phenotype of Scer\GAL4^Toll-6-D42, TFAM^UAS.cCa

(Cagin et al., 2015)

sima^HMS00833/Scer\GAL4^Toll-6-D42 is a suppressor of short lived phenotype of Scer\GAL4^Toll-6-D42, TFAM^UAS.cCa

(Cagin et al., 2015)

Scer\GAL4^VGlut1-OK371/sima^HMS00833 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^VGlut1-OK371, TFAM^UAS.cCa

(Cagin et al., 2015)

sima^HMS00833, Scer\GAL4^Toll-6-D42 is a suppressor | partially of visible | adult stage phenotype of ATPsynCF6^KK108581, Scer\GAL4^Toll-6-D42

(Cagin et al., 2015)

Scer\GAL4^nSyb.PU/sima^HMS00833 is a suppressor of abnormal locomotor behavior | female | adult stage phenotype of Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS

(Cagin et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/sima^HMS00833 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^VGlut1-OK371, TFAM^UAS.cCa

(Cagin et al., 2015)

sima^HMS00833, Scer\GAL4^Toll-6-D42 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of ATPsynCF6^KK108581, Scer\GAL4^Toll-6-D42

(Cagin et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

sima^HMS00833/Scer\GAL4^Toll-6-D42 is a suppressor of wing blade phenotype of Scer\GAL4^Toll-6-D42, TFAM^UAS.cCa

(Cagin et al., 2015)

Scer\GAL4^VGlut1-OK371/sima^HMS00833 is a suppressor of presynaptic active zone | third instar larval stage phenotype of Scer\GAL4^VGlut1-OK371, TFAM^UAS.cCa

(Cagin et al., 2015)

sima^HMS00833, Scer\GAL4^Toll-6-D42 is a suppressor | partially of wing blade phenotype of ATPsynCF6^KK108581, Scer\GAL4^Toll-6-D42

(Cagin et al., 2015)

Scer\GAL4^nSyb.PU/sima^HMS00833 is a suppressor of wing blade phenotype of Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS

(Cagin et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/sima^HMS00833 is a non-suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^VGlut1-OK371, TFAM^UAS.cCa

(Cagin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced climbing ability phenotype observed in park²⁵ mutant adults or in flies expressing sima^HMS00833 under the control of Scer\GAL4^da.PU is (partially) mutually suppressible.

The reduced climbing ability as well as the wing inflation defects characteristic for adult flies expressing Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nSyb.PU are significantly suppressed by co-expression of sima^HMS00833; while only the wing inflation defects but not the climbing deficiency observed flies expressing ATPsynCF6^KK108581 driven by Scer\GAL4^Toll-6-D42 are significantly improved by co-expression of sima^HMS00833.

The adult locomotion and wing inflation defects as well as the reduced lifespan characteristic for flies expressing TFAM^Scer\UAS.cCa under the control of Scer\GAL4^Toll-6-D42 are significantly improved by co-expression of sima^HMS00833. The loss of mitochondria at the larval neuromuscular junction induced by Scer\GAL4^VGlut-OK371-driven expression of TFAM^Scer\UAS.cCa is not affected by co-expression of sima^HMS00833, while the reduction in the number of active zones at the NMJ is fully rescued.

(Cagin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33895