FB2021_05, released October 15, 2021

Allele: Dmel\Vps15HMS00908

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General Information
Symbol
Dmel\Vps15HMS00908
Species
D. melanogaster
Name
FlyBase ID
FBal0257582
Feature type
allele
Associated gene
Dmel\Vps15
Associated Insertion(s)
Carried in Construct
P{TRiP.HMS00908}
Key Links
Genomic Maps
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - RNAi
(Ni et al., 2010.12.1)
in vitro construct - regulatory fusion
(Ni et al., 2010.12.1)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{TRiP.HMS00908}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UAS regulatory sequences drive expression of a short inverted repeat.

      (Ni et al., 2010.12.1)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      Vps15
      Also carries
      loxP
      Associated Sequence Features
      dsRNA-HMS00908
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Vps15HMS00908 under the control of Scer\GAL4Myo31DF-NP0001 results in defective midgut degradation after pupariation, with significantly increased size of the gastric caeca 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

      Expression of Vps15HMS00908 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

      (Xu et al., 2015)

      Females heterozygous for ird1HMS00908 and MTD-Gal4 (consists of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16) crossed to ird1HMS00908 males results in: 69% hatching; non-maternal effect.

      (Sopko et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Vps15HMS00908
      (Billes et al., 2018)
      ird1HMS00908
      Name Synonyms
      Secondary FlyBase IDs
        References (5)