FlyBase Allele Report: Dmel\Rheb[HMS00923]

General Information

Symbol

Dmel\Rheb^HMS00923

Species

D. melanogaster

Name

FlyBase ID

FBal0257596

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00923}

Also Known As

UAS-Rheb-RNAi, UAS-Rheb^RNAi, Rheb RNAi, HMS00923, UAS-Rheb RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00923}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00923

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates syndrome

modeled by RpS23^R67K

(Recasens-Alvarez et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Third instar larvae expressing Rheb^HMS00923 under the control of Scer\GAL4^AB1 show severely smaller salivary gland cells, as compared to controls.

(Sharma et al., 2019)

Expression of Rheb^HMS00923 driven by Scer\GAL4^{Act5C.Switch.PR} (in males fed RU486 during adulthood, to avoid developmental effects) significantly extends lifespan compared to controls.

(Parkhitko et al., 2016)

Scer\GAL4^bs.PM-mediated expression of Rheb^HMS00923 results in decreased terminal cell branching and induction of long, branched autocellular, seamed tubes extend well beyond the terminal cell nucleus.

(Francis and Ghabrial, 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rheb^HMS00923, Scer\GAL4^AB1 has decreased cell size | third instar larval stage phenotype, non-enhanceable by PIP4K²⁹

(Sharma et al., 2019)

NOT suppressed by

Statement

Reference

Rheb^HMS00923, Scer\GAL4^AB1 has decreased cell size | third instar larval stage phenotype, non-suppressible by PIP4K²⁹

(Sharma et al., 2019)

Suppressor of

Statement

Reference

Rheb^HMS00923, Scer\GAL4^Act5C.PI is a suppressor of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, dlg1¹⁴

(Cong et al., 2021)

Scer\GAL4^ci.PC/Rheb^HMS00923 is a suppressor | partially of increased cell death | somatic clone | larval stage phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

NOT Suppressor of

Statement

Reference

Rheb^HMS00923, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rheb^HMS00923, Scer\GAL4^AB1 has fat cell | third instar larval stage phenotype, non-enhanceable by PIP4K²⁹

(Sharma et al., 2019)

NOT suppressed by

Statement

Reference

Rheb^HMS00923, Scer\GAL4^AB1 has fat cell | third instar larval stage phenotype, non-suppressible by PIP4K²⁹

(Sharma et al., 2019)

Suppressor of

Statement

Reference

Rheb^HMS00923, Scer\GAL4^Act5C.PI is a suppressor of eye-antennal disc | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, dlg1¹⁴

(Cong et al., 2021)

Scer\GAL4^ci.PC/Rheb^HMS00923 is a suppressor | partially of imaginal disc phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

NOT Suppressor of

Statement

Reference

Rheb^HMS00923, Scer\GAL4^elav-C155 is a non-suppressor of terminal bouton phenotype of Scer\GAL4^elav-C155, Vap33^P58S.cRa.UAS

(Deivasigamani et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rheb^HMS00923 does not suppress the enlarged bouton phenotype seen when Vap-33A^{P58S.cRa.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33966