FlyBase Allele Report: Dmel\slmb[HMS00946]

General Information

Symbol

Dmel\slmb^HMS00946

Species

D. melanogaster

Name

FlyBase ID

FBal0257616

Feature type

allele

Associated gene

Dmel\slmb

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00946}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00946}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00946

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

increased cell death, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

wing, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

wing disc, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

Detailed Description

Statement

Reference

The expression of slmb^HMS00946 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

Scer\GAL4^sd.PU-mediated expression of slmb^HMS00946 results in wings that are improperly formed and reduced in size.

Scer\GAL4^sd.PU-mediated expression of slmb^HMS00946 induces cell death in wing discs.

(Fereres et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, slmb^HMS00946 has abnormal size phenotype, enhanceable by RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU

(Fereres et al., 2013)

Enhancer of

Statement

Reference

slmb^HMS00946, Scer\GAL4^sd.PU is an enhancer of abnormal size phenotype of RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU

(Fereres et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd.PU, slmb^HMS00946 has wing phenotype, enhanceable by RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU

(Fereres et al., 2013)

Enhancer of

Statement

Reference

slmb^HMS00946, Scer\GAL4^sd.PU is an enhancer of wing phenotype of RYBP^RNAi.UAS.cGa, Scer\GAL4^sd.PU

(Fereres et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^sd.PU-mediated expression of both slmb^HMS00946 and RYBP^{dsRNA.Scer\UAS.cGa} results in a more severe wing phenotype than expression of either transgene alone.

(Fereres et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33986