Open Close
General Information
Symbol
Dmel\maskHMS01045
Species
D. melanogaster
Name
FlyBase ID
FBal0257709
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-mask RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing maskHMS01045 under the control of Scer\GAL4ey.PU induces a small eye phenotype.

Expression under the control of Scer\GAL4en.PU induces substantial apoptosis in the wing disc.

Expression under the control of Scer\GAL4repo.PU induces significant apoptosis in the larval brain.

Expression under the control of Scer\GAL4moody.PU induces a compromised blood-brain barrier, as dye injected into the body cavity is able to diffuse into the adult retina or third instar larval brain. In agreement, larval subperineurial glial cells show disrupted/discontinuous septate junctions (Dlg immunostaining) and significant decreases in nuclear size and ploidy, as compared to controls. There is also a significant decrease in larval brain volume only at higher expression conditions (i.e. at 25[o]C but not at 18[o]C), as compared to controls.

Expressing maskHMS01045 under the control of Scer\GAL4how-24B results in the formation of significantly more autophagosomes and fewer autolysosomes (assessed by the red:green fluorescence ration of Atg8-GFP-mCherry puncta) in muscles throughout third instar larval stage. There is no change in pupal thoracic morphology and body size.

Flies expressing maskHMS01045 under the control of Scer\GAL4how-24B are pupal stage lethal.

Ectopic expression of maskHMS01045 under the control of Scer\GAL4how-24B results in defects in the mitochondrial connectivity in third instar larvae compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference

maskHMS01045, Scer\GAL4how-24B is a non-enhancer of pupal thorax | heat sensitive phenotype of Prosβ61.B.UAS, Scer\GAL4how-24B

maskHMS01045/Scer\GAL4GMR.PU is a non-enhancer of eye phenotype of Atg1UAS.cSa

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of maskHMS01045 RNAi suppresses the short lived phenotype of park25/parkΔ21 transheterozygote adults.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
maskHMS01045
Name Synonyms
Secondary FlyBase IDs
    References (4)