Open Close
General Information
Symbol
Dmel\kccHMS01058
Species
D. melanogaster
Name
FlyBase ID
FBal0257722
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of kccHMS01058 under the control of Scer\GAL4Tsp42Ec leads to reduced acidity in the acidic region of the larval midgut, but no significant effect on larval development or survival under normal conditions, as compared with controls. Mutant larvae exposed to high dietary KCl, but not NaCl, exhibit decreased survival, as compared with controls.

Expression of kccHMS01058 under the control of any of the following drivers mushroom body drivers induces bang-sensitive paralytic behavior: Scer\GAL4Tab2-201Y, Scer\GAL4Mef2.247, Scer\GAL4ey-OK107 or Scer\GAL4c747. Scer\GAL4ey-OK107>kccHMS01058 flies have increased brain volume compared to controls. Giant fiber and lateral pace-making neurons are enlarged compared to controls. The ellipsoid body driver Scer\GAL4Aph-4-c507 and the motor neuron driver Scer\GAL4RapGAP1-OK6 cause lethality.

Expression of kccHMS01058 in glial cells under the control of Scer\GAL4repo.PU results in substantial bang-sensitive paralytic behavior. The threshold to evoked seizure-like discharges in these flies is reduced compared to controls and brain volume is increased by approximately 1.3-fold. Whereas in wild type brains mushroom body neuronal cell bodies and calyces are wrapped and interlaced with glial membranes, these associations are largely absent in Scer\GAL4repo.PU>kccHMS01058 flies. Neuropile glia do not envelop the calyx when glia express kccHMS01058 during development. Peripheral nerve swelling is also seen; third instar larval abdominal nerves display an increase in the average never cross-sectional area compared to controls. This phenotype is completely penetrant.

Expression of kccHMS01058 in subperineurial glia (SPG) cells under the control of Scer\GAL4Gli-rL82 or Scer\GAL4moody.PS results in 100% and 65% bang-sensitive paralytic behavior respectively. Additional phenotypes are seen in the kccHMS01058>Scer\GAL4Gli-rL82 flies. Flies often display malformed wings and are especially sensitive to behavioral seizure-like activity. Gentle handling of the vial is sufficient to trigger seizures and the threshold to evoked seizure-like discharges is reduced compared to controls.

Expression of kccHMS01058 in cortex glia surrounding neuronal cell bodies under the control of Scer\GAL4Akap200-NP2222 results in substantial bang-sensitive paralytic behavior. Bang-sensitive paralytic behavior is also seen when kccHMS01058 is expressed using Scer\GAL4nrv2.PS (cortex and neuropile glia), Scer\GAL4Mz709 (ensheathing glia) and Scer\GAL4alrm.PD (astrocyte-like glia).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
kccHMS01058
Name Synonyms
Secondary FlyBase IDs
    References (5)