FlyBase Allele Report: Dmel\brat[HMS01121]

General Information

Symbol

Dmel\brat^HMS01121

Species

D. melanogaster

Name

FlyBase ID

FBal0257784

Feature type

allele

Associated gene

Dmel\brat

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01121}

Also Known As

UAS-brat RNAi, UAS-brat^RNAi, brat RNAi, UAS-brat-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01121}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01121

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Hussain et al., 2017)

model of brain cancer

CEA

(Hofstetter et al., 2024)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is ameliorated by Pepck1^GD9451

(Hussain et al., 2017)

is ameliorated by Mfe2^KK101732

(Hussain et al., 2017)

is ameliorated by Gpdh1^GD14102

(Hussain et al., 2017)

is ameliorated by Mdh1^GD11625

(Hussain et al., 2017)

is ameliorated by Men^GL01234

(Hussain et al., 2017)

model of brain cancer

is ameliorated by Spt5^HMS00153

(Hofstetter et al., 2024)

is ameliorated by Heatr1^GD7096

(Diaz et al., 2024)

is ameliorated by Myc^GD1419

(Diaz et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of brat^HMS01121 under the control of Scer\GAL4^slbo.2.6 does not lead to defects in border cell migration or cluster integrity.

(Raza et al., 2019)

Third instar larval brain tissue expressing brat^HMS01121 under the control of Scer\GAL4^da.PU exhibits overgrowth and no significant difference in cell death levels as compared to controls, and when this brain tissue (or that expressing brat^HMS01121 under the control of Scer\GAL4^Act5C.PI) is transplanted into the adult abdomen of a wild type host fly it exhibits strong tumorigenic growth, high levels of reactive oxygen species, metastasis, and a high proportion of defects in anaphase, as compared to control transplanted tissue.

(Hussain et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^wor.PA, brat^HMS01121 has short lived phenotype, enhanceable by Scer\GAL4^wor.PA/Spt5^UAS.Tag:FLAG

(Hofstetter et al., 2024)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, enhanceable by Scer\GAL4^da.PU/bsk^GD10555

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, enhanceable by Mfe2^KK101732, Scer\GAL4^da.PU

(Hussain et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-enhanceable by G6pd^KK108898/Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-enhanceable by Pepck1^GD9451, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-enhanceable by Scer\GAL4^da.PU/Wwox^KK108459

(Hussain et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, brat^HMS01121 has increased size | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has increased cell number | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has abnormal mitotic cell cycle | adult stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has short lived phenotype, suppressible | partially by Myc^RNAi.UAS.cUa, Scer\GAL4^wor.PA

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has short lived phenotype, suppressible | partially by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has short lived phenotype, suppressible | partially by Spt5^RNAi.Act5C

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal size phenotype, suppressible by G6pd^KK108898/Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal size phenotype, suppressible by Scer\GAL4^da.PU/bsk^GD10555

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal size phenotype, suppressible by Pepck1^GD9451, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal size phenotype, suppressible | partially by Mfe2^KK101732, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal size phenotype, suppressible | partially by Scer\GAL4^da.PU/Wwox^KK108459

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has neoplasia phenotype, suppressible by Pepck1^GD9451, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has neoplasia phenotype, suppressible by Mfe2^KK101732, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^Act5C.PI, brat^HMS01121 has neoplasia phenotype, suppressible by Gpdh1^GD14102, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

Scer\GAL4^Act5C.PI, brat^HMS01121 has neoplasia phenotype, suppressible by Mdh1^GD11625, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

Scer\GAL4^Act5C.PI, brat^HMS01121 has neoplasia phenotype, suppressible by Men^GL01234, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-suppressible by G6pd^KK108898/Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-suppressible by Pepck1^GD9451, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has abnormal cell cycle phenotype, non-suppressible by Scer\GAL4^da.PU/Wwox^KK108459

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has neoplasia phenotype, non-suppressible by G6pd^KK108898/Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has neoplasia phenotype, non-suppressible by Scer\GAL4^da.PU/bsk^GD10555

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has neoplasia phenotype, non-suppressible by Scer\GAL4^da.PU/Wwox^KK108459

(Hussain et al., 2017)

Other

Statement

Reference

G6pd^KK108898, Scer\GAL4^da.PU, brat^HMS01121 has increased cell death phenotype

(Hussain et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^wor.PA, brat^HMS01121 has adult brain phenotype, suppressible | partially by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has adult brain cell body rind phenotype, suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, brat^HMS01121 has type II neuroblast | increased number phenotype, suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Scer\GAL4^wor.PA, Scer\GAL80^ase.PN, brat^HMS01121 has larval brain | larval stage phenotype, suppressible by Delta^HMS01309, Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

Scer\GAL4^Act5C.PI, brat^HMS01121 has larval brain phenotype, suppressible by Mdh1^GD11625, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

Scer\GAL4^Act5C.PI, brat^HMS01121 has larval brain phenotype, suppressible by Men^GL01234, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has larval brain phenotype, suppressible | partially by Pepck1^GD9451, Scer\GAL4^da.PU

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121 has larval brain phenotype, suppressible | partially by Scer\GAL4^da.PU/Wwox^KK108459

(Hussain et al., 2017)

Scer\GAL4^Act5C.PI, brat^HMS01121 has larval brain phenotype, suppressible by Gpdh1^GD14102, Scer\GAL4^Act5C.PI

(Hussain et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^wor.PA, brat^HMS01121 has intermediate neuronal progenitor | decreased number phenotype, non-suppressible by Scer\GAL4^wor.PA/Spt5^HMS00153

(Hofstetter et al., 2024)

Other

Statement

Reference

G6pd^KK108898, Scer\GAL4^da.PU, brat^HMS01121 has larval brain phenotype

(Hussain et al., 2017)

Scer\GAL4^da.PU, brat^HMS01121, bsk^GD10555 has larval brain phenotype

(Hussain et al., 2017)

Mfe2^KK101732, Scer\GAL4^da.PU, brat^HMS01121 has larval brain phenotype

(Hussain et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The larval brain overgrowth exhibited upon expression of brat^HMS01121 under the control of Scer\GAL4^da.PU is suppressed when Zw^KK108898, bsk^GD10555 or Pepck^GD9451 is co-expressed, and partially suppressed when Mfe2^KK101732 or Wwox^KK108459 is co-expressed. Of these co-expressed alleles, none induce increased cell death levels in the larval brain tissue except for co-expression of Zw^KK108898, which leads to a significant increase in cell death. The proportion of defective anaphases seen in larval brains expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is significantly elevated upon co-expression of bsk^GD10555 or Mfe2^KK101732, but is not significantly changed upon co-expression of Zw^KK108898, Pepck^GD9451 or Wwox^KK108459. The tumor growth rate of larval brain explants expressing brat^HMS01121 under the control of Scer\GAL4^da.PU is suppressed upon co-expression of Pepck^GD9451 or Mfe2^KK101732, but not Zw^KK108898, bsk^GD10555 or Wwox^KK108459.

The tumor growth rate of larval brain explants expressing brat^HMS01121 under the control of Scer\GAL4^Act5C.PI is suppressed upon co-expression of Gpdh^GD14102, Mdh1^GD11625, or Men^GL01234.

(Hussain et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34646