FlyBase Allele Report: Dmel\Cam[HMS01318]

General Information

Symbol

Dmel\Cam^HMS01318

Species

D. melanogaster

Name

FlyBase ID

FBal0257911

Feature type

allele

Associated gene

Dmel\Cam

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01318}

Also Known As

UAS-cam RNAi, UAS-Cam-RNAi, TRiP.HMS01318

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01318}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01318

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

Phenotype Manifest In

adult mushroom body gamma-lobe | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

axon | P-stage, with Scer\GAL4^ey-OK107

(Rabinovich et al., 2016)

salivary gland | pupal stage, with Scer\GAL4^fkh.PU

(Nelson et al., 2014)

Detailed Description

Statement

Reference

Expression of Cam^HMS01318 under the control of Scer\GAL4^ey-OK107 results a subset of P-stage flies showing pruning defects and/or precocious regrowth of axons in the mushroom body gamma-lobe, as compared to controls.

(Rabinovich et al., 2016)

Animals expressing Cam^HMS01318 under the control of Scer\GAL4^fkh.PU show a reduction of autophagy in the salivary glands 14 hours after pupariation and salivary gland fragments persist in these animals 24 hours after puparium (no residual salivary gland material is present in wild-type animals at this stage).

(Nelson et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cam^HMS01318/Scer\GAL4^repo is a suppressor of abnormal neurophysiology | third instar larval stage | heat sensitive phenotype of zyd¹

(Melom and Littleton, 2013)

Cam^HMS01318/Scer\GAL4^repo is a suppressor of abnormal behavior | recessive | adult stage | heat sensitive phenotype of zyd¹

(Melom and Littleton, 2013)

Cam^HMS01318, Scer\GAL4^repo is a suppressor of paralytic | adult stage | heat sensitive phenotype of Scer\GAL4^repo, TrpA1^UAS.(B).cKa

(Melom and Littleton, 2013)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Cam^HMS01318, Scer\GAL4^fkh.PU has salivary gland | pupal stage phenotype, non-suppressible by IP3K2^Δ1

(Nelson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in salivary gland autophagy and persistent salivary gland phenotype which is seen in pupae expressing Cam^HMS01318 under the control of Scer\GAL4^fkh.PU is still seen if the animals are also mutant for IP3K2^Δ1.

(Nelson et al., 2014)

The temperature-sensitive seizure phenotype of zyd¹ larvae and adults is completely suppressed by expression of Cam^HMS01318 under the control of Scer\GAL4^repo.

Co-expression of Cam^HMS01318 lengthens the time required to induce paralysis at 30[o]C in flies expressing TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^repo, and the majority of flies are seen to recover from paralysis during incubation at the restrictive temperature.

(Melom and Littleton, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34609