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General Information
Symbol
Dmel\Atg8aHMS01328
Species
D. melanogaster
Name
FlyBase ID
FBal0257921
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Atg8a RNAi, UAS-Atg8aRNAi, Atg8a-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of Atg8aHMS01328 under the control of Scer\GAL4esg.PU leads to a severe increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

The expression of Atg8aHMS01328 under the control of Scer\GAL4Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls.

Expression of Atg8aHMS01328 under the control of Scer\GAL4phm.PO leads to massive accumulation of lipid droplets in the prothoracic gland cells of third instar larvae.

Expression of Atg8aHMS01328 under the control of Scer\GAL4Myo31DF-NP0001 results in significantly delayed midgut degradation after pupariation, with significantly increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, and significantly decreased autophagy levels 2 hrs after puparium formation, as compared to controls.

Expression of Atg8aHMS01328 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

Scer\GAL4Mhc.PW-mediated expression of Atg8aHMS01328 has no effect on lifespan.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Suppressed by
Enhancer of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Atg8aHMS01328 blocks the longevity induced by Scer\GAL4Mhc.PW-mediated expression of dawHMS01110.

Xenogenetic Interactions
Statement
Reference

Co-expression of Atg8aHMS01328 does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Co-expression of Atg8aHMS01328 enhances eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (16)