FlyBase Allele Report: Dmel\Atg16[HMS01347]

General Information

Symbol

Dmel\Atg16^HMS01347

Species

D. melanogaster

Name

FlyBase ID

FBal0257940

Feature type

allele

Associated gene

Dmel\Atg16

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01347}

Also Known As

HMS01347, UAS-Atg16 RNAi, UAS-Atg16^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01347}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01347

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

adult posterior midgut epithelium, with Scer\GAL4^pros-V1

(Nagy et al., 2017)

autophagosome | :chemical conditional | nutrition conditional, with Scer\GAL4^Mef2.PR

(Zirin et al., 2013)

cytoplasmic vesicle | early third instar larval stage, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

embryonic/larval fat body | larval stage, with Scer\GAL4^Cg.PU

(Xu et al., 2015)

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PP

(Pircs et al., 2012)

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

enteroblast | adult stage, with Scer\GAL4^pros-V1

(Nagy et al., 2017)

fat cell | early third instar larval stage | somatic clone, with Scer\GAL4^Cg.PA

(Liu et al., 2018)

female germline stem cell, with Scer\GAL4^nanos.PG

(Taslim et al., 2023)

gastric caecum | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

Detailed Description

Statement

Reference

Adulthood-only expression of Atg16^HMS01347 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

The expression of Atg16^HMS01347 under the control of Scer\GAL4^Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

(Liu et al., 2018)

The expression of Atg16^HMS01347 under the control of Scer\GAL4^pros-V1 leads to a significant increase in the number of pre-enteroendocrine cells, as compared to controls.

(Nagy et al., 2017)

Expression of Atg16^HMS01347 under the control of Scer\GAL4^{Myo31DF-NP0001} results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, but no significant difference in autophagy levels 2 hrs after puparium formation, as compared to controls.

Expression of Atg16^HMS01347 under the control of Scer\GAL4^cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

(Xu et al., 2015)

Expression of Atg16^HMS01347 under the control of Scer\GAL4^Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

(Zirin et al., 2013)

Expression of Atg16^HMS01347 using Scer\GAL4^Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

(Pircs et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Atg16^HMS01347, Scer\GAL4^pros-V1 has increased cell number | adult stage phenotype, suppressible by sli^EY10695, Scer\GAL4^pros-V1

(Nagy et al., 2017)

NOT Suppressor of

Statement

Reference

Atg16^HMS01347, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg16^HMS01347, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg16^HMS01347, Scer\GAL4^pros-V1 has adult posterior midgut epithelium phenotype, suppressible by sli^EY10695, Scer\GAL4^pros-V1

(Nagy et al., 2017)

Atg16^HMS01347, Scer\GAL4^pros-V1 has enteroblast | adult stage phenotype, suppressible by sli^EY10695, Scer\GAL4^pros-V1

(Nagy et al., 2017)

Suppressor of

Statement

Reference

Atg16^HMS01347, Scer\GAL4^Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg16^HMS01347, Scer\GAL4^Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

NOT Suppressor of

Statement

Reference

Atg16^HMS01347, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of pre-enteroendocrine cells in the adult midgut of individuals expressing Atg16^HMS01347 under the control of Scer\GAL4^pros-V1 is suppressed by the co-expression of sli^EY10695.

(Nagy et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34358

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01347}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg16^HMS01347

(Billes et al., 2018)

CG31033^HMS01347

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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