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General Information
Symbol
Dmel\Atg16HMS01347
Species
D. melanogaster
Name
FlyBase ID
FBal0257940
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
HMS01347, UAS-Atg16 RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a short inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of Atg16HMS01347 under the control of Scer\GAL4esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

The expression of Atg16HMS01347 under the control of Scer\GAL4pros-V1 leads to a significant increase in the number of pre-enteroendocrine cells, as compared to controls.

Expression of Atg16HMS01347 under the control of Scer\GAL4Myo31DF-NP0001 results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, but no significant difference in autophagy levels 2 hrs after puparium formation, as compared to controls.

Expression of Atg16HMS01347 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

Expression of Atg16HMS01347 under the control of Scer\GAL4Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

Expression of Atg16HMS01347 using Scer\GAL4Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of pre-enteroendocrine cells in the adult midgut of individuals expressing Atg16HMS01347 under the control of Scer\GAL4pros-V1 is suppressed by the co-expression of sliEY10695.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Atg16HMS01347
CG31033HMS01347
Name Synonyms
Secondary FlyBase IDs
    References (10)