FlyBase Allele Report: Dmel\Uvrag[HMS01357]

General Information

Symbol

Dmel\Uvrag^HMS01357

Species

D. melanogaster

Name

FlyBase ID

FBal0257950

Feature type

allele

Associated gene

Dmel\Uvrag

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01357}

Also Known As

uvrag^shR, UVRAG RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01357}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01357

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Nagy et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of colorectal cancer

is ameliorated by Stat92E^HJ

(Nagy et al., 2016)

is ameliorated by bsk^DN.UAS.cUa

(Nagy et al., 2016)

is ameliorated by Stat92E^JF01265

(Nagy et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

abnormal immune response, with Scer\GAL4^NP5130

(Nagy et al., 2016)

abnormal size | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

abnormal wound healing | adult stage, with Scer\GAL4^repo

(Szabó et al., 2023)

increased cell number | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

increased cell size | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

short lived, with Scer\GAL4^NP5130

(Nagy et al., 2016)

Phenotype Manifest In

adult marginal nerve wrapping glial cell, with Scer\GAL4^repo

(Szabó et al., 2023)

adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

endosome | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

enteroblast of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

enterocyte of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Nagy et al., 2016)

intracellular vesicle | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

major mitochondrial derivative, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Politi et al., 2014)

marginal nerve, with Scer\GAL4^repo

(Szabó et al., 2023)

mitochondrial derivative | fertilized egg stage, with Scer\GAL4^{VP16.mat.αTub67C}

(Ben-Hur et al., 2024)

Detailed Description

Statement

Reference

Expressing Uvrag^HMS01357 under the control of Scer\GAL4^GMR71G10 does not induce mushroom body gamma neuron pruning defects.

(Meltzer et al., 2019)

The adulthood-only expression of Uvrag^HMS01357 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) lead to significant increases in the thickness and in the number of cells in the adult midgut epithelium, as compared to controls; stem cells and enteroblasts are significantly more numerous, are significantly bigger, show a significant increase in mitotic index, show protrusions that extended towards the underlying muscle layer, show a strong increase in endosomes (Rab7-positive) and show a strong decrease in the number of PI3P-positive vesicles (FYVE-labelled puncta), but do not show a significant change in the number of autophagosomes/autolysosomes (Atg8a--labelled puncta), as compared to controls; the expressing stem cells and enteroblasts show signs of failure to differentiate into enterocytes (e.g. retain esg expression and Notch signaling even after becoming polyploid and fail to express the enterocyte marker Myo1A); this expression does not significantly affect the number of enteroendocrine cells in the adult midgut, as compared to controls. These adults are short lived and defecate significantly less, despite of a similar food intake, as compared to controls.

(Nagy et al., 2016)

Embryos derived from females expressing Uvrag^HMS01357 under the simultaneous control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16} show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type. Early fertilised eggs have multivesicular clusters which are largely devoid of microvesicles.

(Politi et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased cell number | adult stage phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased cell number | adult stage phenotype, suppressible by Scer\GAL4^NP5130/Stat92E^JF01265

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4^NP5130/Stat92E^JF01265

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased cell number | adult stage phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased cell number | adult stage phenotype, suppressible by Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased cell number | adult stage phenotype, suppressible by Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by bsk¹/bsk[+]

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by bsk²/bsk[+]

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, suppressible by Stat92E^HJ/Stat92E[+]

(Nagy et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, non-suppressible by wg[+]/wg^l-8

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has increased occurrence of cell division | adult stage phenotype, non-suppressible by wg^l-12/wg[+]

(Nagy et al., 2016)

NOT Suppressor of

Statement

Reference

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of decreased cell number | adult stage phenotype of Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of decreased occurrence of cell division | adult stage phenotype of Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of decreased cell number | adult stage phenotype of Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of decreased occurrence of cell division | adult stage phenotype of Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357/Scer\GAL4^NP5130 is a non-suppressor of decreased cell number | adult stage phenotype of Scer\GAL4^NP5130, pan^ΔN.UAS

(Nagy et al., 2016)

Uvrag^HMS01357/Scer\GAL4^NP5130 is a non-suppressor of decreased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, pan^ΔN.UAS

(Nagy et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has enteroblast of posterior adult midgut epithelium phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by Scer\GAL4^NP5130/Stat92E^JF01265

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Scer\GAL4^NP5130/Stat92E^JF01265

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has enteroblast of posterior adult midgut epithelium phenotype, suppressible by Scer\GAL4^NP5130/Stat92E^JF01265

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has intestinal stem cell of posterior adult midgut epithelium phenotype, suppressible by Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by bsk¹/bsk[+]

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by bsk²/bsk[+]

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, suppressible by Stat92E^HJ/Stat92E[+]

(Nagy et al., 2016)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, non-suppressible by wg[+]/wg^l-8

(Nagy et al., 2016)

Scer\GAL4^NP5130, Uvrag^HMS01357 has adult midgut epithelium phenotype, non-suppressible by wg^l-12/wg[+]

(Nagy et al., 2016)

NOT Suppressor of

Statement

Reference

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of adult midgut epithelium phenotype of Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Atg14^KK100903, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357/Scer\GAL4^NP5130 is a non-suppressor of adult midgut epithelium phenotype of Scer\GAL4^NP5130, pan^ΔN.UAS

(Nagy et al., 2016)

Uvrag^HMS01357/Scer\GAL4^NP5130 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, pan^ΔN.UAS

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of adult midgut epithelium phenotype of Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Uvrag^HMS01357, Scer\GAL4^NP5130 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Atg5^JF02703, Scer\GAL4^NP5130

(Nagy et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased numbers of intestinal stem cells/enteroblast and the increased mitotic index induced in the adult midgut epithelium by the adulthood-only expression of Uvrag^HMS01357 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) are suppressed by the co-expression of either bsk^DN.UAS.cUa or Stat92E^JF01265. The increased mitotic index in the adult midgut epithelium is also suppressed by heterozygosity for either Stat92E⁰⁶³⁴⁶, Stat92E^HJ, bsk¹ or bsk², but not by heterozygosity for wg^l-8 or wg^l-12.

The adulthood-only co-expression of Uvrag^HMS01357 with either pan^ΔN.UAS, Atg5^JF02703 or Atg14^KK100903 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same severe decreased numbers of intestinal stem cells and mitotic figures in the adult midgut epithelium as upon the single expression of pan^ΔN.UAS, Atg5^JF02703 or Atg14^KK100903, rather than the converse increases induced by the single expression of Uvrag^HMS01357.

(Nagy et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34368

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01357}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG6116^HMS01357

UVRAG^HMS01357

(Szabó et al., 2023)

Uvrag^HMS01357

uvrag^shR

(Politi et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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