Allele SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}

General Information
Symbol	SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}	Species	P. Simian virus 40
Name		FlyBase ID	FBal0258003
Feature type	allele	Associated gene	SV40\ST

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Kotadia et al., 2008) in vitro construct - regulatory fusion (Kotadia et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory and P\T promoter sequences drive expression of SV40\ST which is tagged at the C-terminal end with T:Zzzz\FLAG. (Kotadia et al., 2008)
Carried in construct	P{UASp-ST.FLAG} (Kotadia et al., 2008)
Tagged with	T:Zzzz\FLAG (Kotadia et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Kotadia et al., 2008)
Phenotype Manifest In
	centrosome \| supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Kotadia et al., 2008) cleavage furrow, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} (Kotadia et al., 2008)
Detailed Description
	Statement Reference Approximately 70% of embryos expressing SV40\ST[Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] fail to hatch, with most embryos failing to progress beyond the early syncytial and blastoderm cleavage cycles of embryogenesis. Expression of SV40\ST[Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16] causes centrosome overduplication by the first mitotic cycle in embryos, and also causes other spindle assembly defects, including a failure to organise or maintain chromosomes within the spindle. The extra centrosomes are often free and not associated with a mitotic spindle. During cleavage stage divisions, larger astral microtubules containing longer microtubules than normal are seen. Actin frequently fails to assemble into cleavage furrows, but instead organises into clumps near the centrosomes. The cleavage cycles retain synchrony and progress on a similar timescale as control embryos in embryos expressing SV40\ST[Scer\UAS.P\T.T:Zzzz\FLAG] under the control of Scer\GAL4[nos.UTR.T:Hsim\VP16], but some nuclei fail to complete cleavage, causing daughter chromosomes to collapse back together after anaphase and producing polyploid nuclei associated with four centrosomes that fail to enter the next cleavage cycle. (Kotadia et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, enhanceable by mts^XE-2258/mts[+] (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, enhanceable by Pp2A-29B^EP2332/Pp2A-29B[+] (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, enhanceable by PP2A-B'[+]/wdb[+]/wdb^12-1/PP2A-B'^KG01108 (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, enhanceable by tws[+]/tws⁰²⁴¹⁴ (Kotadia et al., 2008)
NOT Enhanced by
Statement Reference SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, non-enhanceable by PP2A-B'[+]/PP2A-B'^KG01108 (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has lethal \| embryonic stage phenotype, non-enhanceable by wdb[+]/wdb^12-1 (Kotadia et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has centrosome \| supernumerary phenotype, enhanceable by mts^XE-2258/mts[+] (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has centrosome \| supernumerary phenotype, enhanceable by Pp2A-29B^EP2332/Pp2A-29B[+] (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has centrosome \| supernumerary phenotype, enhanceable by tws[+]/tws⁰²⁴¹⁴ (Kotadia et al., 2008) SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}, Scer\GAL4^{nos.UTR.T:Hsim\VP16} has centrosome \| supernumerary phenotype, enhanceable by wdb[+]/wdb^12-1/PP2A-B'^KG01108 (Kotadia et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Kotadia et al., 2008, Oncogene 27(49): 6334--6346 PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen. [FBrf0206924]

Allele SV40\STScer\UAS.P\T.T:Zzzz\FLAG

Allele SV40\ST^{Scer\UAS.P\T.T:Zzzz\FLAG}