Allele Dmel\Nrx-1^Δ83

General Information
Symbol	Dmel\Nrx-1^Δ83	Species	D. melanogaster
Name		FlyBase ID	FBal0258010
Feature type	allele	Associated gene	Dmel\Nrx-1

Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Zeng et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Nrx-1^d08766 (Zeng et al., 2007)
Nature of the lesion	Statement Reference Mobilisation of P{XP}Nrx-1[d08766] by Δ2-3 transposase results in a 4066bp deletion. (Zeng et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Zeng et al., 2007, Sun et al., 2011) fertile (with Df(3R)Exel6191) (Zeng et al., 2007) learning defective (Zeng et al., 2007) neurophysiology defective \| adult stage (Tian et al., 2013) phototaxis defective \| adult stage (Tian et al., 2013) short lived (Zeng et al., 2007) short lived (with Nrx-1^Scer\UAS.cZa), with Scer\GAL4^elav-C155 (Zeng et al., 2007) viable (Zeng et al., 2007, Sun et al., 2011) viable (with Df(3R)Exel6191) (Zeng et al., 2007)
Phenotype Manifest In
	synapse (Zeng et al., 2007) synapse (with Df(3R)Exel6191) (Zeng et al., 2007)
Detailed Description
	Statement Reference Mutant flies show a reduced phototactic response to light compared to wild type. Mutant photoreceptors show approximately 10,000-fold light sensitivity compared to wild type. Mutant flies do not show a blue light-induced prolonged depolarization afterpotential. Mutant photoreceptors have a normal endoplasmic reticulum structure at 65% of pupal development. However, at 79% of pupal development, the endoplasmic reticulum appears expanded. (Tian et al., 2013) neuroanatomy defective, enhanceable { neuroligin[KO70]/+ } (Sun et al., 2011) Nrx-1[Δ83] and Nrx-1[Δ83]/Df(3R)Exel6191 mutants are viable and fertile, with no obvious developmental defects. Nrx-1[Δ83] mutants are short-lived. At 37 days, only 32.5% of Nrx-1[Δ83] mutants are alive, compared with 82.5% of wild-type and 79% of rescue flies (Scer\GAL4[elav-C155]/+; Nrx-1[Scer\UAS.cZa]/+; Nrx-1[Δ83]). Nrx-1[Δ83] and Nrx-1[Δ83]/Df(3R)Exel6191 mutants exhibit fewer synapses than controls (approximately half). Associative learning is reduced in Nrx-1[Δ83] mutants (testing for their ability to associate odors with a fructose reward in an individual-animal assay). (Zeng et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Nrx-1[+]/Nrx-1^Δ83 is an enhancer of neuroanatomy defective phenotype of neuroligin^KO70 (Sun et al., 2011) Nrx-1^Δ83 is an enhancer of neuroanatomy defective phenotype of neuroligin^KO70 (Sun et al., 2011)
Other
Statement Reference Nrx-1^Δ83, neuroligin^KO70 has lethal phenotype (Sun et al., 2011)
Phenotype Manifest In
Enhanced by
Statement Reference Nrx-1^Δ83 has bouton \| second instar larval stage phenotype, enhanceable by neuroligin[+]/neuroligin^KO70 (Sun et al., 2011) Nrx-1^Δ83 has synapse \| second instar larval stage phenotype, enhanceable by neuroligin[+]/neuroligin^KO70 (Sun et al., 2011)
Enhancer of
Statement Reference Nrx-1[+]/Nrx-1^Δ83 is an enhancer of bouton \| second instar larval stage phenotype of neuroligin^KO70 (Sun et al., 2011) Nrx-1[+]/Nrx-1^Δ83 is an enhancer of synapse \| second instar larval stage phenotype of neuroligin^KO70 (Sun et al., 2011) Nrx-1^Δ83 is an enhancer of bouton \| second instar larval stage phenotype of neuroligin^KO70 (Sun et al., 2011) Nrx-1^Δ83 is an enhancer of synapse \| second instar larval stage phenotype of neuroligin^KO70 (Sun et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Homozygous neuroligin[KO70];Nrx-1[Δ83] double mutants die during the second instar larval stage. In early second-instar larvae, neuroligin[KO70];Nrx-1[Δ83] double mutants show a significant reduction in bouton numbers compared with wild-type as in neuroligin[KO70] single mutants. However, the double mutants appear to demonstrate more severe synaptic morphology defects than in the single mutants. Consistent with this, neuroligin[KO70];Nrx-1[Δ83] double mutants show more severe defects in locomotion than either single mutant. Flies homozygous for neuroligin[KO70] and heterozygous for Nrx-1[Δ83] are viable and show more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than neuroligin[KO70] single mutants. Nrx-1[Δ83] homozygous mutants in a neuroligin[KO70] heterozygous background exhibit more severe defects in neuromuscular morphology at both muscles 6/7 and muscle 4 than Nrx-1[Δ83] single mutants. (Sun et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Nrx-1^Δ83 is rescued by Nrx-1^Δ83/Scer\GAL4^GMR.PU (Tian et al., 2013, Tian et al., 2013) Nrx-1^Δ83 is rescued by Scer\GAL4^elav-C155/Nrx-1^Scer\UAS.cZa (Zeng et al., 2007)
Not rescued by	Nrx-1^Δ83 is not rescued by Scer\GAL4^Tab2-201Y/Nrx-1^Scer\UAS.cZa (Zeng et al., 2007)
Rescues	Nrx-1^Δ83/Scer\GAL4^GMR.PU rescues Nrx-1^Δ83 (Tian et al., 2013, Tian et al., 2013)
Comments	The reduced light sensitivity of Nrx-1[Δ83] photoreceptors is rescued by expression of Nrx-1[Scer\UAS.cTa] under the control of Scer\GAL4[GMR.PU]. (Tian et al., 2013) Synapse number is rescued in Scer\GAL4[elav-C155]/+; Nrx-1[Scer\UAS.cZa]/+; Nrx-1[Δ83] flies. Longevity is rescued in Scer\GAL4[elav-C155]/+; Nrx-1[Scer\UAS.cZa]/+; Nrx-1[Δ83] flies. Associative learning is rescued inScer\GAL4[elav-C155]/+; Nrx-1[Scer\UAS.cZa]/+; Nrx-1[Δ83] flies. (Zeng et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Nrx-1^Δ83 nrx^Δ83 (Tian et al., 2013)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Tian et al., 2013, Neuron 77(2): 311--322 Neurexin Regulates Visual Function via Mediating Retinoid Transport to Promote Rhodopsin Maturation. [FBrf0220614] Sun et al., 2011, J. Neurosci. 31(2): 687--699 Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction. [FBrf0212770] Zeng et al., 2007, FEBS Lett. 581(13): 2509--2516 Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila. [FBrf0201905]

Allele Dmel\Nrx-1Δ83

Allele Dmel\Nrx-1^Δ83