FlyBase Allele Report: Dmel\mEFG1[Del-EY2]

General Information

Symbol

Dmel\mEFG1^Del-EY2

Species

D. melanogaster

Name

FlyBase ID

FBal0258154

Feature type

allele

Associated gene

Dmel\mEFG1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Trivigno and Haerry, 2011)

Progenitor genotype

P{EPgy2}CG30265^EY05983

(Trivigno and Haerry, 2011)

Caused by aberration

Df(2L)ico^Del-EY2

Cytology

Description

Imprecise excision of P{EPgy2}EY05983 resulting in the deletion of 3663 nucleotides including the C-terminal RNA binding domain of ico and a portion of CG13784. The deletion creates a stop codon 19 residues past C367 of ico.

(Trivigno and Haerry, 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of first instar larval stage (with mEFG1^GA1)

(Trivigno and Haerry, 2011)

lethal - all die before end of larval stage (with mEFG1^Del-EY1)

(Trivigno and Haerry, 2011)

some die during first instar larval stage (with mEFG1^GA1)

(Trivigno and Haerry, 2011)

some die during third instar larval stage (with mEFG1^Del-EY1)

(Trivigno and Haerry, 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous ico^Del-EY2 animals die as early third instar larvae.

ico^Del-EY1/ico^Del-EY2 animals die as early third instar larvae.

ico^GA1/ico^Del-EY2 animals die as malformed first instar larvae.

Homozygous clones of ico^Del-EY2 mutant cells do not cause any pattern defects in the eye.

(Trivigno and Haerry, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mEFG1^Del-EY2/mEFG1^Del-EY1 has lethal | early third instar larval stage phenotype, suppressible by Scer\GAL4^da.G32/Scer\GAL4^arm.PS/Hsap\GFM1^UAS.cTa

(Trivigno and Haerry, 2011)

mEFG1^Del-EY2/mEFG1^Del-EY1 has lethal | early third instar larval stage phenotype, suppressible | partially by Hsap\GFM1^N174S.UAS/Scer\GAL4^da.G32/Scer\GAL4^arm.PS

(Trivigno and Haerry, 2011)

mEFG1^Del-EY2/mEFG1^Del-EY1 has lethal | early third instar larval stage phenotype, suppressible | partially by Hsap\GFM1^S321P.UAS/Scer\GAL4^da.G32/Scer\GAL4^arm.PS

(Trivigno and Haerry, 2011)

NOT suppressed by

Statement

Reference

mEFG1^Del-EY2/mEFG1^Del-EY1 has lethal | early third instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Scer\GAL4^arm.PS/mRRF2^UAS.cTa

(Trivigno and Haerry, 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of EF-G2^Scer\UAS.cTa under the control of Scer\GAL4^arm.PS and Scer\GAL4^da.G32 fails to rescue the lethality of ico^Del-EY1/ico^Del-EY2.

(Trivigno and Haerry, 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\GFM1^Scer\UAS.cTa under the control of Scer\GAL4^arm.PS and Scer\GAL4^da.G32 rescues the lethality of ico^Del-EY1/ico^Del-EY2.

Expression of Hsap\GFM1^{N174S.Scer\UAS} under the control of Scer\GAL4^arm.PS and Scer\GAL4^da.G32 partially rescues the lethality of ico^Del-EY1/ico^Del-EY2. The first animals rescued by this transgene hatch three to five days later than heterozygous controls.

Expression of Hsap\GFM1^{S321P.Scer\UAS} under the control of Scer\GAL4^arm.PS and Scer\GAL4^da.G32 partially rescues the lethality of ico^Del-EY1/ico^Del-EY2. The first animals rescued by this transgene hatch at least five days later than heterozygous controls.

(Trivigno and Haerry, 2011)

Complementation and Rescue Data

Rescued by

mEFG1^Del-EY2/mEFG1^Del-EY1 is rescued by mEFG1^UAS.cTa/Scer\GAL4^da.G32/Scer\GAL4^arm.PS

(Trivigno and Haerry, 2011)

mEFG1^Del-EY2/mEFG1^Del-EY1 is rescued by mEFG1^UAS.GFP/Scer\GAL4^da.G32/Scer\GAL4^arm.PS

(Trivigno and Haerry, 2011)

mEFG1^Del-EY2/mEFG1^Del-EY1 is rescued by Scer\GAL4^da.G32/Scer\GAL4^arm.PS/mEFG1^ΔCstop.UAS

(Trivigno and Haerry, 2011)

Not rescued by

mEFG1^Del-EY2/mEFG1^Del-EY1 is not rescued by mEFG1^G538E.UAS/Scer\GAL4^da.G32/Scer\GAL4^arm.PS

(Trivigno and Haerry, 2011)