FlyBase Allele Report: Dmel\drpr[I.UAS]

General Information

Symbol

Dmel\drpr^I.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0258200

Feature type

allele

Associated gene

Dmel\drpr

Associated Insertion(s)

Carried in Construct

P{UAS-drpr.I}

Also Known As

UAS-drpr-I, UAS-Draper-I, UAS-drpr

Key Links

Transgenic product class

cDNA

(McPhee et al., 2010)

wild_type

(McPhee et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(McPhee et al., 2010)

wild_type

(McPhee et al., 2010)

Mutagen

in vitro construct

(Logan et al., 2012, McPhee et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-drpr.I}

Cytology

Description

UASt regulatory sequences drive expression of isoform I of drpr. Isoform I of drpr encodes an immunoreceptor tyrosine-based activation motif (ITAM) (corresponding to GH24127).

(Logan et al., 2012)

UASt regulatory sequences drive expression of drpr isoform I full length cDNA.

(McPhee et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

drpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.E693G.UAS.VTR}

(Ray et al., 2017)

ameliorates fragile X syndrome

modeled by Fmr1^Δ50M

(Vita et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | adult stage, with Scer\GAL4^GR1

(Etchegaray et al., 2012)

Phenotype Manifest In

nurse cell, with Scer\GAL4^GR1

(Etchegaray et al., 2012)

Detailed Description

Statement

Reference

Expression of drpr^I.Scer\UAS under the control of Scer\GAL4^GR1 in otherwise wild type and well-fed flies induces nurse cell death. Nurse cells condense and fragment in stage 8 and 9 egg chambers, but instead of engulfing nurse cells, the follicle cells initially thin out. Engulfment of the nurse cell debris eventually begins and proceeds normally.

(Etchegaray et al., 2012)

Glial expression of drpr^I.Scer\UAS (under the control of Scer\GAL4^repo) does not affect glial engulfment of axonal debris.

(Logan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

drpr^I.UAS, Scer\GAL4^repo.PU, NimC4^UAS.GFP is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of repo⁰³⁷⁰²

(Shklyar et al., 2014)

NOT Suppressor of

Statement

Reference

drpr^I.UAS/Scer\GAL4^repo.PU is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of repo⁰³⁷⁰²

(Shklyar et al., 2014)

Other

Statement

Reference

Scer\GAL4^GR1, bsk^DN.UAS, drpr^I.UAS has lethal phenotype

(Etchegaray et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

drpr^I.UAS, Scer\GAL4^repo.PU, NimC4^UAS.GFP is a suppressor of glial cell phenotype of repo⁰³⁷⁰²

(Shklyar et al., 2014)

NOT Suppressor of

Statement

Reference

drpr^I.UAS/Scer\GAL4^repo.PU is a non-suppressor of glial cell phenotype of repo⁰³⁷⁰²

(Shklyar et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of drpr^I.Scer\UAS under the control of Scer\GAL4^repo.PU does not rescue the glial phagocytosis defects seen in repo⁰³⁷⁰² mutant embryos; the morphology and localisation of the glia still appear abnormal, and there is an increase in apoptotic particles outside of the glial cells.

Co-expression of NimC4^{Scer\UAS.T:Avic\GFP} and drpr^I.Scer\UAS under the control of Scer\GAL4^repo.PU fully rescues the phagocytosis defects seen in repo⁰³⁷⁰² mutant embryos.

(Shklyar et al., 2014)

Overexpression of drpr^I.Scer\UAS (under the control of Scer\GAL4^repo) in a EcR^{B1-ΔC655.W650A.Scer\UAS} background fails to transform astrocytes into phagocytes at puparium formation.

(Tasdemir-Yilmaz and Freeman, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^C587/drpr^I.UAS rescues drpr^Δ5

(Zohar-Fux et al., 2022)

drpr^I.UAS/Scer\GAL4^GR1 rescues drpr^Δ5

(Etchegaray et al., 2012)

drpr^I.UAS/Scer\GAL4^repo rescues drpr^Δ5

(Logan et al., 2012)

Scer\GAL4^fkh.PH/drpr^I.UAS rescues drpr^Δ5

(McPhee et al., 2010)

Comments

Expression of drpr^I.Scer\UAS in the follicle cells under the control of Scer\GAL4^GR1 rescues the enlargement and nurse cell debris uptake defects seen in starvation-induced degenerating egg chambers in drpr^Δ5 mutant flies. Unlike in drpr^Δ5 alone, no premature follicle cell death is seen.

(Etchegaray et al., 2012)

Glial expression of drpr^I.Scer\UAS rescues the engulfment defects found in degenerating ORN axons in drpr^Δ5 mutants.

(Logan et al., 2012)

Expression of drpr^I.Scer\UAS under the control of Scer\GAL4^fkh.PH in drpr^Δ5 mutant animals restores salivary gland degradation, and only 10% of animals display persistent gland material.

(McPhee et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67035

y¹ w^*; P{UAS-drpr.I}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

drpr^I.Scer\UAS

drpr^I.UAS

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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