Allele Dmel\Gr64f^Scer\UAS.cJa

General Information
Symbol	Dmel\Gr64f^Scer\UAS.cJa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Jiao	FlyBase ID	FBal0258334
Feature type	allele	Associated gene	Dmel\Gr64f

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Jiao et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Gr64f. (Jiao et al., 2008)
Carried in construct	P{UAS-Gr64f.J} (Jiao et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Gr64f[Scer\UAS.cJa] in Df(3L)ΔGr64 mutants under the control of Scer\GAL4[Gr5a.1] fully restores trehalose sensation in a behavioural assay. Similarly to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1]display high frequencies of action potentials in response to trehalose. (Jiao et al., 2008)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference In contrast to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] do not show a behavioural preference for sucrose over fructose. In contrast to wild-type flies, the mutant flies display only a minimal increase in action potentials in response to sucrose. Df(3L)ΔGr64 mutants expressing both Gr64f[Scer\UAS.cJa] and Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] show a behavioural preference for sucrose over fructose that is very similar to that of wild-type flies. The wild-type frequency of action potentials in response to sucrose is also restored in these animals. In contrast to wild-type flies, Df(3L)ΔGr64 mutant flies expressing Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] display only a minimal increase in action potentials in response to maltose and they do not show a behavioural preference for maltose over fructose. Df(3L)ΔGr64 mutants expressing both Gr64f[Scer\UAS.cJa] and Gr64a[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] show a behavioural preference for maltose over fructose that is very similar to that of wild-type flies. The wild-type frequency of action potentials in response to maltose is also restored in these animals. Expression of Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] partially restores the preference for glucose over fructose and action potentials in response to glucose in Df(3L)ΔGr64 mutants. Co-expression of Gr64a[Scer\UAS.cJa] and Gr64f[Scer\UAS.cJa] under the control of Scer\GAL4[Gr5a.1] restores normal preference for glucose over fructose and wild-type frequencies of glucose dependent actin potentials. (Jiao et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Gr64f^Scer\UAS.cJa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Jiao
Secondary FlyBase IDs

References ( 1 )
Research paper	Jiao et al., 2008, Curr. Biol. 18(22): 1797--1801 Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila. [FBrf0206328]

Allele Dmel\Gr64fScer\UAS.cJa

Allele Dmel\Gr64f^Scer\UAS.cJa