FlyBase Allele Report: Dmel\hppy[UAS.cLa]

General Information

Symbol

Dmel\hppy^UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0258364

Feature type

allele

Associated gene

Dmel\hppy

Associated Insertion(s)

Carried in Construct

P{UAS-hppy.L}

Key Links

Transgenic product class

wild_type

(Lam et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lam et al., 2010)

Mutagen

in vitro construct

(Lam et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-hppy.L}

Cytology

Description

UAS regulatory sequences drive expression of hppy coding sequences for the hppy-RB transcript.

(Lam et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

hppy

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^e22c

(Lam et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

(Lam et al., 2010)

some die during pupal stage, with Scer\GAL4^da.G32

(Lam et al., 2010)

Phenotype Manifest In

wing, with Scer\GAL4^da.G32

(Lam et al., 2010)

wing, with Scer\GAL4^e22c

(Lam et al., 2010)

wing margin bristle, with Scer\GAL4^e22c

(Lam et al., 2010)

wing vein, with Scer\GAL4^e22c

(Lam et al., 2010)

Detailed Description

Statement

Reference

Flies expressing hppy^Scer\UAS.cLa under the control of Scer\GAL4^da.G32 that survive pupation exhibit a reduced wing area. There is a clear decrease in the total number of bristle cells on the wing, indicating cell loss.

Overexpression of hppy^Scer\UAS.cLa driven by Scer\GAL4^e22c in the posterior compartment of the developing wing disc results in the appearance of TUNEL-positive cells, indicating apoptotic cell death. Resultant adult wings display wing vein defects and a clear reduction in the posterior area of the wing.

Ectopic expression of hppy^Scer\UAS.cLa driven by Scer\GAL4^e22c results in caspase activation in the posterior compartment of the developing wing discs.

(Lam et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^e22c, hppy^UAS.cLa has increased cell death phenotype, suppressible by Buffy^UAS.cQa, Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has increased cell death phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has increased cell death phenotype, suppressible by gig^UAS.cTa/Scer\GAL4^e22c/Tsc1^UAS.cTa

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Lam et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^e22c, hppy^UAS.cLa has increased cell death phenotype, non-suppressible by Thor^TA.UAS/Scer\GAL4^e22c

(Lam et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^e22c, hppy^UAS.cLa has wing phenotype, suppressible by gig^UAS.cTa/Scer\GAL4^e22c/Tsc1^UAS.cTa

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing margin bristle phenotype, suppressible by gig^UAS.cTa/Scer\GAL4^e22c/Tsc1^UAS.cTa

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing vein phenotype, suppressible by gig^UAS.cTa/Scer\GAL4^e22c/Tsc1^UAS.cTa

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing margin bristle phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing vein phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^e22c

(Lam et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^e22c, hppy^UAS.cLa has wing phenotype, non-suppressible by Thor^TA.UAS/Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing margin bristle phenotype, non-suppressible by Thor^TA.UAS/Scer\GAL4^e22c

(Lam et al., 2010)

Scer\GAL4^e22c, hppy^UAS.cLa has wing vein phenotype, non-suppressible by Thor^TA.UAS/Scer\GAL4^e22c

(Lam et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic caspase activation in the posterior compartment of the developing wing discs resulting from expression of hppy^Scer\UAS.cLa under the control of Scer\GAL4^e22c is moderately suppressed through co-expression of Buffy^Scer\UAS.cQa.

Inhibition of the JNK pathway through expression of bsk^DN.Scer\UAS under the control of Scer\GAL4^e22c suppresses the cell death induced by ectopic hppy^Scer\UAS.cLa expression.

Co-expression of Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa (together forming the tuberous sclerosis complex) under the control of Scer\GAL4^e22c does not affect cell death in the posterior compartment of the wing discs seen upon expression of hppy^Scer\UAS.cLa.

Expression of Thor^TA.Scer\UAS under the control of Scer\GAL4^e22c does not affect cell death in the posterior compartment of the wing discs seen upon expression of hppy^Scer\UAS.cLa.

(Lam et al., 2010)

Xenogenetic Interactions

Statement

Reference

Ectopic caspase activation in the posterior compartment of the developing wing discs resulting from expression of hppy^Scer\UAS.cLa under the control of Scer\GAL4^e22c is completely suppressed through co-expression of BacA\p35^Scer\UAS.cHa.

(Lam et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hppy^Scer\UAS.cLa

hppy^UAS.cLa

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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