FlyBase Allele Report: Dmel\Irbp[EX8]

General Information

Symbol

Dmel\Irbp^EX8

Species

D. melanogaster

Name

FlyBase ID

FBal0258426

Feature type

allele

Associated gene

Dmel\Irbp

Associated Insertion(s)

Carried in Construct

Also Known As

ku70^Ex8

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Johnson-Schlitz et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{lacW}l(3)S005818^S005818

(Johnson-Schlitz et al., 2007)

Cytology

Nature of the lesion

Statement

Reference

Deletion mutation.

(Johnson-Schlitz et al., 2007)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | recessive

(Johnson-Schlitz et al., 2007)

radiation sensitive | recessive

(Johnson-Schlitz et al., 2007)

viable

(Merigliano et al., 2017)

visible | recessive

(Johnson-Schlitz et al., 2007)

Phenotype Manifest In

adult thoracic sensillum | increased number

(Johnson-Schlitz et al., 2007)

macrochaeta | increased number

(Johnson-Schlitz et al., 2007)

Detailed Description

Statement

Reference

Irbp^EX8/Irbp^EX8 third instar larval brains exhibit a similar frequency of cells with chromosome aberrations and no telomeric fusions compared to controls.

(Merigliano et al., 2017)

Heterozygotes have a normal lifespan.

(Tamura et al., 2011)

Homozygotes for Irbp^EX8 are hypersensitive to the chemical mutagen methyl methanesulfonate.

Homozygotes for Irbp^EX8 are mildly hypersensitive to gamma irradiation.

Many of the homozygous Irbp^EX8 flies have an excess of macrochaetae (large thoracic bristles) that are deformed or reduced in size.

(Johnson-Schlitz et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Irbp[+]/Irbp^EX8 is an enhancer | female limited of short lived phenotype of Hsap\HTT^103Q.Ex1.UAS, Scer\GAL4^RapGAP1-OK6

(Tamura et al., 2011)

Other

Statement

Reference

Irbp^EX8, tws⁴³⁰ has lethal - all die before end of larval stage phenotype

(Merigliano et al., 2017)

Hsap\HTT^103Q.Ex1.UAS, Irbp^EX8/Irbp^7B2, Scer\GAL4^RapGAP1-OK6 has abnormal locomotor behavior phenotype

(Tamura et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Irbp^EX8/Irbp^EX8 is a suppressor of nuclear chromosome | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Irbp^EX8/Irbp^EX8 is a suppressor of embryonic/larval brain | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Irbp^{fTRG00107.sfGFP-TVPTBF}, Irbp^EX8/Irbp^EX8 is a suppressor | partially of nuclear chromosome | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Irbp^{fTRG00107.sfGFP-TVPTBF}, Irbp^EX8/Irbp^EX8 is a suppressor | partially of embryonic/larval brain | third instar larval stage phenotype of tws⁴³⁰

(Merigliano et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Heterozygosity for Irbp^EX8 enhances the shortened lifespan phenotype caused by expression of Hsap\HTT^{103Q.Ex1.Scer\UAS} under the control of Scer\GAL4^GMR.long. This enhancing effect is seen in females but not males.

Flies expressing Hsap\HTT^{103Q.Ex1.Scer\UAS} under the control of Scer\GAL4^Rapgap1-OK6 in a Irbp^7B2/Irbp^EX8 background show an enhanced age-dependent decline in climbing ability compared to control flies.

(Tamura et al., 2011)

Complementation and Rescue Data

Rescued by

Irbp^EX8 is rescued by Irbp^+t13

(Johnson-Schlitz et al., 2007)

Comments

Irbp^+t13 rescues the chemical mutagen and gamma irradiation hypersensitivity of Irbp^EX8 mutants.

Irbp^+t13 rescues the excess macrochaeta phenotype of Irbp^EX8 mutants.

(Johnson-Schlitz et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ex8

(Johnson-Schlitz et al., 2007)

Irbp^EX8

(Beaucher et al., 2012, Johnson-Schlitz et al., 2007)

Ku70^EX8

(Tamura et al., 2011)

ku70^EX8

(Johnson-Schlitz et al., 2007)

ku70^Ex8

(Merigliano et al., 2017, White and Lambowitz, 2012)

ku70^ex8

(Yu and McVey, 2010)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close