Allele Dmel\Gug^{75QN.Scer\UAS}

General Information
Symbol	Dmel\Gug^{75QN.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0258445
Feature type	allele	Associated gene	Dmel\Gug

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - site directed mutagenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Nisoli et al., 2010) in vitro construct - site directed mutagenesis (Nisoli et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a modified form of Gug carrying a 75Q stretch in the middle of a polyprolin domain. (Nisoli et al., 2010)
Carried in construct	P{UAS-Atro.75QN} (Nisoli et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Eaat1.PR (Nisoli et al., 2010) lethal, with Scer\GAL4^elav.PU (Nisoli et al., 2010)
Phenotype Manifest In
	autophagic vacuole, with Scer\GAL4^GMR.PU (Nisoli et al., 2010) eye, with Scer\GAL4^GMR.PU (Nisoli et al., 2010) eye, with Scer\GAL4^ninaE.PT (Nisoli et al., 2010) eye photoreceptor cell, with Scer\GAL4^ninaE.PT (Nisoli et al., 2010) eye photoreceptor cell \| drug conditional, with Scer\GAL4^GMR.PU (Nisoli et al., 2010) retina \| temperature conditional, with Scer\GAL4^GMR.PU (Nisoli et al., 2010)
Detailed Description
	Statement Reference Expression of Gug[75QN.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PU] results in progressive depigmentation. At 14 days old, degenerating photoreceptor cells display large autophagosomes. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[ninaE.PT] results in degeneration, characterised by loss of photoreceptor cells. Expression of Gug[75QN.Scer\UAS] in the glia under the control of Scer\GAL4[Eaat1.PR] results in reduced animal viability. Expression of Gug[75QN.Scer\UAS] in the neurons under the control of Scer\GAL4[elav.PU] results in reduced animal viability. Expression of Gug[75QN.Scer\UAS] in the photoreceptor neurons under the control of Scer\GAL4[GMR.PU] in flies fed on 1μM rapamycin for 2 or 7 days both result in a reduction in the number of photoreceptor cells per ommatidium. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[GMR.PU], aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue. (Nisoli et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Gug^{75QN.Scer\UAS}, Scer\GAL4^GMR.PU has autophagic vacuole phenotype, enhanceable by Tor^TED.Scer\UAS, Scer\GAL4^GMR.PU (Nisoli et al., 2010) Gug^{75QN.Scer\UAS}, Scer\GAL4^GMR.PU has retina \| temperature conditional phenotype, enhanceable by Atg1^GS10797, Scer\GAL4^GMR.PU (Nisoli et al., 2010) Gug^{75QN.Scer\UAS}, Scer\GAL4^GMR.PU has retina \| temperature conditional phenotype, enhanceable by Atg5^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PU (Nisoli et al., 2010) Gug^{75QN.Scer\UAS}, Scer\GAL4^GMR.PU has retina \| temperature conditional phenotype, enhanceable by Tor^TED.Scer\UAS, Scer\GAL4^GMR.PU (Nisoli et al., 2010)
Suppressed by
Statement Reference Gug^{75QN.Scer\UAS}, Scer\GAL4^GMR.PU has retina \| temperature conditional phenotype, suppressible by DnaJ-1^Scer\UAS.cKa, Scer\GAL4^GMR.PU (Nisoli et al., 2010)
NOT Suppressor of
Statement Reference Gug^{75QN.Scer\UAS}/Scer\GAL4^GMR.PU is a non-suppressor of eye \| somatic clone phenotype of Atg1^Δ3D (Nisoli et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Tor[TED.Scer\UAS] and Gug[75QN.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PU] results in the formation of gigantic autophagolysosomes. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[GMR.PU], aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is suppressed by co-expression of DnaJ-1[Scer\UAS.cKa]. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[GMR.PU], aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg5[dsRNA.Scer\UAS]. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[GMR.PU], aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Tor[TED.Scer\UAS]. Expression of Gug[75QN.Scer\UAS] in the adult eye under the control of Scer\GAL4[GMR.PU], aged at 29[o]C for 28 days, results in a significant degeneration of retinal tissue, which is enhanced by co-expression of Atg1[GS10797]. (Nisoli et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Gug^{75QN.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Nisoli et al., 2010, Cell Death Differ. 17(10): 1577--1587 Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy. [FBrf0212035]

Allele Dmel\Gug75QN.Scer\UAS

Allele Dmel\Gug^{75QN.Scer\UAS}