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General Information
Symbol
Hsap\HTT152Q.Ex1.UAS.EGFP
Species
H. sapiens
Name
FlyBase ID
FBal0258488
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Hsap\HTT exon 1 containing a 152Q expansion, fused to EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 has no apparent effect on larval da neuron viability.

Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 results in the formation of cytoplasmic aggregates in the axon and cell body, and in addition aggregates are seen throughout the extensive dendritic arbors of the da neurons. There is also some limited aggregation in the nucelus.

Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in larval body wall sensory neurons under the control of Scer\GAL4RluA1 does not affect dendrite elaboration in da neurons of the dorsal cluster. Dendrites fully cover the field and project towards the dorsal midline to meet the contralateral dendrites, and also fully extend to segment boundaries.

The speed of locomotion is not different in Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP expressing larva (driven by Scer\GAL4RluA1) compared to wild-type controls. The trajectory of larval locomotion is also not affected.

Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP in the developing eye using the Scer\GAL4GMR.PF driver does not produce a strong external eye phenotype.

Expression of Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 in the developing eye using the Scer\GAL4GMR.PF driver produces a degenerative eye phenotype characterized by loss of pigment cells and an increased occurrence of dark necrotic spots on the external eye.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of DnaJ-1Scer\UAS.cKa in the developing eye under the control of Scer\GAL4GMR.PF suppresses the eye phenotype found in flies mutant for both Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1.

Overexpression of RhebScer\UAS.cPa in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF, with a severe rough eye phenotype and enhancement of pigment cell loss.

Expression of CG1109GD7195 in the developing eye under the control of Scer\GAL4GMR.PF strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.

Expression of CG5537GD11686 in the developing eye under the control of Scer\GAL4GMR.PF suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.

Expression of Nup160GD11413 in the developing eye under the control of Scer\GAL4GMR.PF strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF. There is a clear decrease in the number of necrotic fly eyes in these progeny.

Expression of hiwGD14104 in the developing eye under the control of Scer\GAL4GMR.PF enhances the loss of pigment seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP
Hsap\HTT152Q.Ex1.UAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (3)