Allele Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}	Species	H. sapiens
Name		FlyBase ID	FBal0258488
Feature type	allele	Associated gene	Hsap\HTT

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Doumanis et al., 2009) in vitro construct - regulatory fusion (Doumanis et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\HTT exon 1 containing a 152Q expansion, fused to T:Avic\GFP[EGFP]. (Doumanis et al., 2009)
Carried in construct	P{UAS-Htt.152Q-EGFP} (Doumanis et al., 2009, Nishimura et al., 2010)
Tagged with	T:Avic\GFP^EGFP (Doumanis et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	melanotic necrosis, with Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009) melanotic necrosis, with Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Phenotype Manifest In
	eye, with Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009) eye, with Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009) pigment cell, with Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009) pigment cell, with Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Detailed Description
	Statement Reference Expression of Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] in larval body wall sensory neurons under the control of Scer\GAL4[RluA1] has no apparent effect on larval da neuron viability. Expression of Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] in larval body wall sensory neurons under the control of Scer\GAL4[RluA1] results in the formation of cytoplasmic aggregates in the axon and cell body, and in addition aggregates are seen throughout the extensive dendritic arbors of the da neurons. There is also some limited aggregation in the nucelus. Expression of Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] in larval body wall sensory neurons under the control of Scer\GAL4[RluA1] does not affect dendrite elaboration in da neurons of the dorsal cluster. Dendrites fully cover the field and project towards the dorsal midline to meet the contralateral dendrites, and also fully extend to segment boundaries. The speed of locomotion is not different in Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] expressing larva (driven by Scer\GAL4[RluA1]) compared to wild-type controls. The trajectory of larval locomotion is also not affected. (Nishimura et al., 2010) Expression of Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] in the developing eye using the Scer\GAL4[GMR.PF] driver does not produce a strong external eye phenotype. Expression of Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] in the developing eye using the Scer\GAL4[GMR.PF] driver produces a degenerative eye phenotype characterized by loss of pigment cells and an increased occurrence of dark necrotic spots on the external eye. (Doumanis et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has melanotic necrosis phenotype, suppressible by CG1109^GD7195, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has melanotic necrosis phenotype, suppressible by CG5537^GD11686, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has melanotic necrosis phenotype, suppressible by DnaJ-1^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has melanotic necrosis phenotype, suppressible by Nup160^GD11413, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by hiw^GD14104, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Rheb^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, enhanceable by hiw^GD14104, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, enhanceable by Rheb^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Suppressed by
Statement Reference Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by CG1109^GD7195, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by CG5537^GD11686, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by DnaJ-1^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Nup160^GD11413, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible by CG1109^GD7195, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible by CG5537^GD11686, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible by DnaJ-1^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF has pigment cell phenotype, suppressible by Nup160^GD11413, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Enhancer of
Statement Reference Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}/Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, hiw^GD14101 (Doumanis et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of DnaJ-1[Scer\UAS.cKa] in the developing eye under the control of Scer\GAL4[GMR.PF] suppresses the eye phenotype found in flies mutant for both Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1]. Overexpression of Rheb[Scer\UAS.cPa] in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF], with a severe rough eye phenotype and enhancement of pigment cell loss. Expression of CG1109[GD7195] in the developing eye under the control of Scer\GAL4[GMR.PF] strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF]. There is a clear decrease in the number of necrotic fly eyes in these progeny. Expression of CG5537[GD11686] in the developing eye under the control of Scer\GAL4[GMR.PF] suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF]. There is a clear decrease in the number of necrotic fly eyes in these progeny. Expression of Nup160[GD11413] in the developing eye under the control of Scer\GAL4[GMR.PF] strongly suppresses the loss of pigment seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF]. There is a clear decrease in the number of necrotic fly eyes in these progeny. Expression of hiw[GD14104] in the developing eye under the control of Scer\GAL4[GMR.PF] enhances the loss of pigment seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF]. (Doumanis et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Nishimura et al., 2010, J. Neurogenet. 24(4): 194--206 Selection of Behaviors and Segmental Coordination During Larval Locomotion Is Disrupted by Nuclear Polyglutamine Inclusions in a New Drosophila Huntington's Disease-Like Model. [FBrf0212327] Doumanis et al., 2009, PLoS ONE 4(9): e7275 RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. [FBrf0209128]

Allele Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP

Allele Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}