Allele Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}

General Information
Symbol	Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}	Species	H. sapiens
Name		FlyBase ID	FBal0258493
Feature type	allele	Associated gene	Hsap\HTT

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Doumanis et al., 2009) in vitro construct - regulatory fusion (Doumanis et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Hsap\HTT coding sequences corresponding to exon 1 with a 98Q expansion, tagged at the N-terminal with T:Zzzz\nls1 and the C-terminal with T:Avic\GFP[EGFP]. (Doumanis et al., 2009)
Carried in construct	P{UAS-nls.Htt.98Q-EGFP} (Doumanis et al., 2009)
Tagged with	T:Avic\GFP^EGFP (Doumanis et al., 2009) T:Zzzz\nls1 (Doumanis et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4^elav-C155 (Doumanis et al., 2009)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] in the developing nervous system under the control of Scer\GAL4[elav-C155] generates a number of visible aggregates in the four week old male brain. (Doumanis et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^elav-C155 has neuroanatomy defective phenotype, enhanceable by Rheb^Scer\UAS.cPa, Scer\GAL4^elav-C155 (Doumanis et al., 2009)
Suppressed by
Statement Reference Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^elav-C155 has neuroanatomy defective phenotype, suppressible by CG5537^GD11686, Scer\GAL4^elav-C155 (Doumanis et al., 2009) Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^elav-C155 has neuroanatomy defective phenotype, suppressible by Nup160^GD11413, Scer\GAL4^elav-C155 (Doumanis et al., 2009)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of CG5537[GD11686] in a Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] mutant brain (with both transgenes under the control of Scer\GAL4[elav-C155]) significantly decreases the average number and size of brain aggregate inclusions compared to flies expressing Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] alone. Co-expression of Nup160[GD11413] in a Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] mutant brain (with both transgenes under the control of Scer\GAL4[elav-C155]) significantly decreases the average number and size of brain aggregate inclusions compared to flies expressing Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] alone. Co-expression of Rheb[Scer\UAS.cPa] in a Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] mutant brain (with both transgenes under the control of Scer\GAL4[elav-C155]) does not significantly increase the average number of brain aggregate inclusions compared to flies expressing Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] alone, but does increase the average aggregate size. (Doumanis et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Doumanis et al., 2009, PLoS ONE 4(9): e7275 RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. [FBrf0209128]

Allele Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1

Allele Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}