FB2025_01 , released February 20, 2025
Allele: Dmel\Drp1UAS.cDa
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General Information
Symbol
Dmel\Drp1UAS.cDa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Deng
FlyBase ID
FBal0258603
Feature type
allele
Associated gene
Dmel\Drp1
Associated Insertion(s)
Carried in Construct
P{UAS-Drp1.D}
Also Known As
uas-drp1, UAS Drp1, DRP1OE
Key Links
Transgenic product class
cDNA
(Deng et al., 2008)
wild_type
(Deng et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Deng et al., 2008)
wild_type
(Deng et al., 2008)
Mutagen
in vitro construct
(Deng et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Drp1.D}
Cytology
Description

UAS regulatory sequences drive expression of Drp1 cDNA clone AT04516.

(Deng et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Drp1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Drp1UAS.cDa under the control of Scer\GAL4e33C results in the third instar larval lymph gland showing an increase in crystal cells in the primary lobe.

      (Ray et al., 2021)

      Expressing Drp1UAS.cDa under the control of Scer\GAL4Cg.PA leads to small and fragmented mitochondria in the early third instar larval fat body.

      (Liu et al., 2020)

      Expressing Drp1UAS.cDa under the control of Scer\GAL4Mef2.PR leads to a small but significant decrease in adult climbing ability, but does not have an obvious effect in the thorax muscles; muscle mitochondria also do not show obvious defects.

      Expressing Drp1UAS.cDa under the control of Scer\GAL4Cg.PA does not seem to lead to mitochondrial defects in the third instar larval fat body (as assessed by MitoGFP).

      (Zhou et al., 2019)

      Expression of Drp1UAS.cDa under the control of Scer\GAL4Mef2.PR has no significant effect on larval locomotor activity or adult eclosion rate.

      (Wang et al., 2016)

      Flies expressing Drp1Scer\UAS.cDa under the control of Scer\GAL4Bx-MS1096 does not result in any wing defects.

      (Ratnaparkhi, 2013)

      Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4Mef2.PR results in mitochondrial fragmentation (they are smaller and rounder than normal) in the indirect flight muscles.

      (Deng et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      Statement
      Reference

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Cg.PA is a suppressor of abnormal size | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Scer\GAL4Cg.PA, PuHMC04085/Drp1UAS.cDa is a suppressor | partially of abnormal size | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor of abnormal size | adult stage phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Drp1UAS.cDa/Scer\GAL4GMR.PF is a suppressor of abnormal neuroanatomy | dominant | adult stage | progressive phenotype of trpP365

      (Huang et al., 2016)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor | partially of partially lethal - majority die phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)

      Drp1UAS.cDa, Scer\GAL4Act5C.PU is a suppressor | partially of partially lethal - majority die phenotype of PolG1KK100485, Scer\GAL4Act5C.PU

      (Humphrey et al., 2012)

      Drp1UAS.cDa, Scer\GAL4Act5C.PU is a suppressor | partially of some die during larval stage phenotype of PolG1KK100485, Scer\GAL4Act5C.PU

      (Humphrey et al., 2012)

      Drp1UAS.cDa, Scer\GAL4Act5C.PU is a suppressor | partially of some die during pupal stage phenotype of PolG1KK100485, Scer\GAL4Act5C.PU

      (Humphrey et al., 2012)

      Drp1UAS.cDa/Scer\GAL4Mef2.PR is a suppressor of visible phenotype of Pink15

      (Deng et al., 2008)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      Drp1UAS.cDa/Scer\GAL4GMR.PU is an enhancer of eye photoreceptor cell | adult stage | decreased number phenotype of Hsap\MAPTGMR.Ex.PJ

      (Zhou et al., 2024)

      Drp1UAS.cDa, Scer\GAL4GMR.PU is an enhancer of eye photoreceptor cell | adult stage | decreased number phenotype of Scer\GAL4GMR.PU, TrabdUAS.Tag:V5

      (Zhou et al., 2024)

      Scer\GAL4e33C/Drp1UAS.cDa is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of asrij9

      (Ray et al., 2021)

      Scer\GAL4e33C/Drp1UAS.cDa is an enhancer of primary lobe of embryonic/larval lymph gland | third instar larval stage phenotype of asrij9

      (Ray et al., 2021)

      Drp1UAS.cDa, Scer\GAL4dome-PG14 is an enhancer of embryonic/larval crystal cell | third instar larval stage | increased number phenotype of Scer\GAL4dome-PG14, asrijGD2167

      (Ray et al., 2021)

      Drp1UAS.cDa, Scer\GAL4dome-PG14 is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of Scer\GAL4dome-PG14, asrijGD2167

      (Ray et al., 2021)

      Drp1UAS.cDa/nanosHMS00785, Scer\GAL4Cg.PA is an enhancer of mitochondrion | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa/nanosHMS00785, Scer\GAL4Cg.PA is an enhancer of embryonic/larval fat body | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4Bx-MS1096, fogUAS.cRa

      (Ratnaparkhi, 2013)

      Drp1UAS.cDa, Scer\GAL4Bx-MS1096 is an enhancer of wing | male limited phenotype of Scer\GAL4Bx-MS1096, fogUAS.cRa

      (Ratnaparkhi, 2013)
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      Drp1UAS.cDa/Scer\GAL4Mhc.PU is a suppressor of indirect flight muscle cell phenotype of Pink1B9

      (Li et al., 2023)

      Drp1UAS.cDa/Scer\GAL4Mhc.PU is a suppressor of mitochondrial crista | adult stage phenotype of Pink1B9

      (Li et al., 2023)

      Drp1UAS.cDa/Scer\GAL4Mhc.PU is a suppressor of mitochondrion | adult stage phenotype of Pink1B9

      (Li et al., 2023)

      Drp1UAS.cDa/Scer\GAL4Mhc.PU is a suppressor of mitochondrial membrane | adult stage phenotype of Pink1B9

      (Li et al., 2023)

      Drp1UAS.cDa, Scer\GAL4Appl.G1a is a suppressor of mitochondrion | larval stage phenotype of Hsap\SNCAUAS.cFa, Scer\GAL4Appl.G1a

      (Krzystek et al., 2021)

      Scer\GAL4e33C/Drp1UAS.cDa is a suppressor | partially of mitochondrion | third instar larval stage phenotype of asrij9

      (Ray et al., 2021)

      Scer\GAL4e33C/Drp1UAS.cDa is a suppressor | partially of prohemocyte | third instar larval stage phenotype of asrij9

      (Ray et al., 2021)

      Scer\GAL4e33C/Drp1UAS.cDa is a suppressor | partially of embryonic/larval lymph gland | third instar larval stage phenotype of asrij9

      (Ray et al., 2021)

      Drp1UAS.cDa, Scer\GAL4Cg.PA is a suppressor of mitochondrion | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Cg.PA is a suppressor of embryonic/larval fat body | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor of adult somatic muscle cell of thorax | adult stage phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of DhprHMC05508, Scer\GAL4Mef2.PR

      (Zhou et al., 2019)

      Scer\GAL4Cg.PA, PuHMC04085/Drp1UAS.cDa is a suppressor | partially of mitochondrion | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Scer\GAL4Cg.PA, PuHMC04085/Drp1UAS.cDa is a suppressor | partially of embryonic/larval fat body | third instar larval stage phenotype of DhprHMC05508, Scer\GAL4Cg.PA

      (Zhou et al., 2019)

      Drp1UAS.cDa/Scer\GAL4GMR.PF is a suppressor of rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype of trpP365

      (Huang et al., 2016)

      Drp1UAS.cDa, Scer\GAL4Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4Mef2.PR, cluGD13926

      (Wang et al., 2016)

      Drp1UAS.cDa/Scer\GAL4how-24B is a suppressor of mitochondrion phenotype of park25

      (Deng et al., 2008)

      Drp1UAS.cDa/Scer\GAL4how-24B is a suppressor of indirect flight muscle cell phenotype of park25

      (Deng et al., 2008)

      Drp1UAS.cDa/Scer\GAL4Mef2.PR is a suppressor of mitochondrion phenotype of Pink15

      (Deng et al., 2008)

      Drp1UAS.cDa/Scer\GAL4Mef2.PR is a suppressor of wing phenotype of Pink15

      (Deng et al., 2008)

      Drp1UAS.cDa/Scer\GAL4Mef2.PR is a suppressor of indirect flight muscle cell phenotype of Pink15

      (Deng et al., 2008)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Drp1Scer\UAS.cDa (positive regulator of mitofission) driven by Scer\GAL4GMR.PF suppresses retinal degeneration (loss of rhabdomeres from photoreceptors) in 8 day old trpP365/+ flies.

      (Huang et al., 2016)

      The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing cluGD13926 under the control of Scer\GAL4Mef2.PR can be suppressed to a varying degree by co-expression of Drp1UAS.cDa.

      (Wang et al., 2016)

      Overexpression of Drp1Scer\UAS.cDa under the control of Scer\GAL4Bx-MS1096, enhances the wing phenotype of Scer\GAL4Bx-MS1096>fogScer\UAS.cRa flies, with males appearing similar to Scer\GAL4Bx-MS1096>fogScer\UAS.cRa males, with corresponding females showing strong wing defects ranging from relatively mild margin defects to strong wing defects that are similar to those in males.

      (Ratnaparkhi, 2013)

      Expression of Drp1Scer\UAS.cDa partially suppresses the lethality seen when tamKK100485 is expressed under the control of Scer\GAL4Act5C.PU.

      (Humphrey et al., 2012)

      Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4elav.PU does not rescue mitochondrial morphology in Df(3L)Pmi-PGRP-LD flies.

      (Rival et al., 2011)

      Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4Mef2.PR suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink15 mutants. The defects in wing posture and indirect flight muscle degeneration phenotypes are also suppressed.

      Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4how-24B suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of park25 mutants. The indirect flight muscle degeneration phenotype is also suppressed.

      (Deng et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference

      Overexpression of Drp1Scer\UAS.cDa in flies also expressing Scer\GAL4elav.PU>Hsap\HTTQ93.ex1p.Scer\UAS results in an about 23% further reduction in lifespan.

      (Khalil et al., 2015)
      Complementation and Rescue Data
      Rescues

      Drp1UAS.cDa/Scer\GAL4Cg.PA rescues Drp1HMC03230

      (Zhou et al., 2019)

      Drp1UAS.cDa/Scer\GAL4elav.PU rescues Drp12/Drp11

      (Rival et al., 2011)
      Comments

      Expression of Drp1Scer\UAS.cDa under the control of Scer\GAL4elav.PU rescues mitochondrial morphology in Drp11/Drp12 flies.

      (Rival et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Drp1Scer\UAS.cDa
      Drp1UAS.cDa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Deng
      Secondary FlyBase IDs
        References (27)