Allele Dmel\p38b^d27

General Information
Symbol	Dmel\p38b^d27	Species	D. melanogaster
Name		FlyBase ID	FBal0258646
Feature type	allele	Associated gene	Dmel\p38b

Allele class	amorphic allele - molecular evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Cully et al., 2010)
Mutagen	P-element activity (Cully et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}p38b^GE3128 (Cully et al., 2010)
Nature of the lesion	Statement Reference Imprecise excision of P{EP}p38b[GE3128] resulting in removal of 862 nucleotides, including the first exon and half of the second exon, which includes the initiating methionine and the first 539 nucleotides of the coding sequence. (Cully et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| adult stage \| recessive (Cully et al., 2010) heat stress response defective \| recessive (Cully et al., 2010) melanotic mass phenotype \| larval stage (Seisenbacher et al., 2011) melanotic mass phenotype \| larval stage \| nutrition conditional (Seisenbacher et al., 2011) osmotic stress response defective \| recessive (Cully et al., 2010) partially lethal - majority die (Seisenbacher et al., 2011) partially lethal - majority die \| recessive (Cully et al., 2010) small body \| adult stage \| recessive (Cully et al., 2010) starvation stress response defective \| recessive (Cully et al., 2010)
Phenotype Manifest In
	embryonic/larval hindgut \| larval stage \| posterior (Seisenbacher et al., 2011) embryonic/larval hindgut \| larval stage \| posterior \| nutrition conditional (Seisenbacher et al., 2011)
Detailed Description
	Statement Reference p38b[d27] mutant larvae exhibit a melanotic phenotype under normal food conditions. A "black dot" is seen in the posterior hindgut. The number of larvae exhibiting melanotic masses increases under salt stress conditions. Survival rate is reduced compared to controls in both normal conditions and in response to salt stress. (Seisenbacher et al., 2011) Approximately 20% of homozygous larvae die before pupariation, with an additional 20% of homozygous pupae failing to emerge. Lethality is accentuated by raising larvae on low-nutrient medium: only 20% of homozygotes survive to adulthood under these conditions. Few adults survive when homozygous larvae are raised on food containing high salt. Adult homozygotes are abnormally sensitive to hydrogen peroxide, dry starvation, and high temperatures. Bristle density on the wings of p38b[d27] flies is increased by approximately 10%, indicating a decrease in cell size. (Cully et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference p38b^d27 has melanotic mass phenotype \| larval stage phenotype, enhanceable by MAPk-Ak2^Δ43 (Seisenbacher et al., 2011)
NOT Enhanced by
Statement Reference p38b^d27 has melanotic mass phenotype \| larval stage \| nutrition conditional phenotype, non-enhanceable by MAPk-Ak2^Δ43 (Seisenbacher et al., 2011) p38b^d27 has partially lethal - majority die phenotype, non-enhanceable by MAPk-Ak2^Δ43 (Seisenbacher et al., 2011)
Suppressed by
Statement Reference p38b^d27 has melanotic mass phenotype \| larval stage phenotype, suppressible \| partially by Scer\GAL4^Act.PU/Mpk2^{DPTD.Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
NOT suppressed by
Statement Reference p38b^d27 has melanotic mass phenotype \| larval stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Mpk2^{Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
Other
Statement Reference Mpk2¹, p38b^d27 has decreased cell size \| somatic clone \| cell autonomous phenotype (Cully et al., 2010) Mpk2¹, p38b^d27 has lethal \| third instar larval stage phenotype (Cully et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference p38b^d27 has embryonic/larval hindgut \| posterior \| larval stage phenotype, enhanceable by MAPk-Ak2^Δ43 (Seisenbacher et al., 2011)
NOT Enhanced by
Statement Reference p38b^d27 has embryonic/larval hindgut \| posterior \| larval stage \| nutrition conditional phenotype, non-enhanceable by MAPk-Ak2^Δ43 (Seisenbacher et al., 2011)
Suppressed by
Statement Reference p38b^d27 has embryonic/larval hindgut \| posterior \| larval stage phenotype, suppressible \| partially by Scer\GAL4^Act.PU/Mpk2^{DPTD.Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
NOT suppressed by
Statement Reference p38b^d27 has embryonic/larval hindgut \| posterior \| larval stage phenotype, non-suppressible by Scer\GAL4^Act.PU/Mpk2^{Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference MAPk-Ak2[Δ43] slightly enhances the melanotic phenotype seen in p38b[d27] larvae under normal food conditions. A "black dot" (BD) is seen in the posterior hindgut in approximately 28% of larvae. However, MAPk-Ak2[Δ43] does not increase the number of p38b[d27] mutant larvae displaying the BD phenotype in salt stress conditions. The semi-lethality seen in p38b[d27] is not enhanced by MAPk-Ak2[Δ43]. Expression of Mpk2[Scer\UAS.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] is unable to suppress the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl. Expression of Mpk2[DPTD.Scer\UAS.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] partially suppresses the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl. (Seisenbacher et al., 2011) p38b[d27], Mpk2[1] double mutants die 96-120 hours after egg lay. p38b[d27] wing disc clones induced in a Mpk2[1] background contain cells that are approximately 15% smaller than heterozygous cells in the same tissue. In contrast, their cell cycle profiles are wild type. (Cully et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	p38b^d27 is rescued by Scer\GAL4^Act.PU/p38b^{Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
Partially rescued by	p38b^d27 is partially rescued by Scer\GAL4^Act.PU/p38b^{Scer\UAS.EPSV.T:Sjap\GST} (Seisenbacher et al., 2011)
Not rescued by	p38b^d27 is not rescued by Scer\GAL4^Act.PU/p38b^{AGF.Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011) p38b^d27 is not rescued by Scer\GAL4^Act.PU/p38b^{KR.Scer\UAS.T:Sjap\GST} (Seisenbacher et al., 2011)
Comments	Expression of p38b[Scer\UAS.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] rescues the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl. Expression of p38b[AGF.Scer\UAS.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] fails to rescue the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with 0.1M NaCl. The larvae die when raised on 0.2M NaCl. Expression of p38b[KR.Scer\UAS.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] fails to rescue the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with 0.1M NaCl. The larvae die when raised on 0.2M NaCl. Expression of p38b[Scer\UAS.EPSV.T:Sjap\GST] under the control of Scer\GAL4[Act.PU] partially rescues the "black dot" phenotype seen in the hindgut of p38b[d27] mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl. (Seisenbacher et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	p38b^d27 (Cully et al., 2010, Seisenbacher et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Seisenbacher et al., 2011, PLoS Genet. 7(8): e1002168 MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic Stress. [FBrf0214607] Cully et al., 2010, Mol. Cell. Biol. 30(2): 481--495 A role for p38 stress-activated protein kinase in regulation of cell growth via TORC1. [FBrf0209636]

Allele Dmel\p38bd27

Allele Dmel\p38b^d27