FlyBase Allele Report: Dmel\p38b[d27]

General Information

Symbol

Dmel\p38b^d27

Species

D. melanogaster

Name

FlyBase ID

FBal0258646

Feature type

allele

Associated gene

Dmel\p38b

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Cully et al., 2010)

Mutagen

P-element activity

(Cully et al., 2010)

Progenitor genotype

P{EP}p38b^GE3128

(Cully et al., 2010)

Cytology

Description

Imprecise excision of P{EP}p38b^GE3128 resulting in removal of 862 nucleotides, including the first exon and half of the second exon, which includes the initiating methionine and the first 539 nucleotides of the coding sequence.

(Cully et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:13,780,680..13,781,541 [+]

Comment:

862 bp deletion resulting from the imprecise excision of P{EP}p38b^GE3128, which removes exon 1, the intron, exon 2 and the first 539 bp of the coding sequence of p38b.

(Cully et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hindgut | larval stage | posterior

(Seisenbacher et al., 2011)

embryonic/larval hindgut | larval stage | posterior | nutrition conditional

(Seisenbacher et al., 2011)

Detailed Description

Statement

Reference

p38b^d27 mutant larvae exhibit a melanotic phenotype under normal food conditions. A "black dot" is seen in the posterior hindgut. The number of larvae exhibiting melanotic masses increases under salt stress conditions. Survival rate is reduced compared to controls in both normal conditions and in response to salt stress.

(Seisenbacher et al., 2011)

Approximately 20% of homozygous larvae die before pupariation, with an additional 20% of homozygous pupae failing to emerge. Lethality is accentuated by raising larvae on low-nutrient medium: only 20% of homozygotes survive to adulthood under these conditions.

Few adults survive when homozygous larvae are raised on food containing high salt.

Adult homozygotes are abnormally sensitive to hydrogen peroxide, dry starvation, and high temperatures.

Bristle density on the wings of p38b^d27 flies is increased by approximately 10%, indicating a decrease in cell size.

(Cully et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

p38b^d27 has melanotic mass phenotype | larval stage phenotype, enhanceable by MAPk-Ak2^Δ43

(Seisenbacher et al., 2011)

NOT Enhanced by

Statement

Reference

p38b^d27 has melanotic mass phenotype | larval stage | nutrition conditional phenotype, non-enhanceable by MAPk-Ak2^Δ43

(Seisenbacher et al., 2011)

p38b^d27 has partially lethal - majority die phenotype, non-enhanceable by MAPk-Ak2^Δ43

(Seisenbacher et al., 2011)

Suppressed by

Statement

Reference

p38b^d27 has melanotic mass phenotype | larval stage phenotype, suppressible | partially by Scer\GAL4^Act.PU/p38a^{DPTD.UAS.Tag:GST}

(Seisenbacher et al., 2011)

NOT suppressed by

Statement

Reference

p38b^d27 has melanotic mass phenotype | larval stage phenotype, non-suppressible by Scer\GAL4^Act.PU/p38a^UAS.Tag:GST

(Seisenbacher et al., 2011)

Other

Statement

Reference

p38a¹, p38b^d27 has lethal | third instar larval stage phenotype

(Cully et al., 2010)

p38a¹, p38b^d27 has decreased cell size | somatic clone | cell autonomous phenotype

(Cully et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

p38b^d27 has embryonic/larval hindgut | larval stage | posterior phenotype, enhanceable by MAPk-Ak2^Δ43

(Seisenbacher et al., 2011)

NOT Enhanced by

Statement

Reference

p38b^d27 has embryonic/larval hindgut | larval stage | posterior | nutrition conditional phenotype, non-enhanceable by MAPk-Ak2^Δ43

(Seisenbacher et al., 2011)

Suppressed by

Statement

Reference

p38b^d27 has embryonic/larval hindgut | posterior | larval stage phenotype, suppressible | partially by Scer\GAL4^Act.PU/p38a^{DPTD.UAS.Tag:GST}

(Seisenbacher et al., 2011)

NOT suppressed by

Statement

Reference

p38b^d27 has embryonic/larval hindgut | posterior | larval stage phenotype, non-suppressible by Scer\GAL4^Act.PU/p38a^UAS.Tag:GST

(Seisenbacher et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

MAPk-Ak2^Δ43 slightly enhances the melanotic phenotype seen in p38b^d27 larvae under normal food conditions. A "black dot" (BD) is seen in the posterior hindgut in approximately 28% of larvae. However, MAPk-Ak2^Δ43 does not increase the number of p38b^d27 mutant larvae displaying the BD phenotype in salt stress conditions. The semi-lethality seen in p38b^d27 is not enhanced by MAPk-Ak2^Δ43.

Expression of Mpk2^{Scer\UAS.T:Sjap\GST} under the control of Scer\GAL4^Act.PU is unable to suppress the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl.

Expression of Mpk2^{DPTD.Scer\UAS.T:Sjap\GST} under the control of Scer\GAL4^Act.PU partially suppresses the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl.

(Seisenbacher et al., 2011)

p38b^d27, Mpk2¹ double mutants die 96-120 hours after egg lay.

p38b^d27 wing disc clones induced in a Mpk2¹ background contain cells that are approximately 15% smaller than heterozygous cells in the same tissue. In contrast, their cell cycle profiles are wild type.

(Cully et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

p38b^d27 is rescued by Scer\GAL4^Act.PU/p38b^UAS.Tag:GST

(Seisenbacher et al., 2011)

Partially rescued by

p38b^d27 is partially rescued by Scer\GAL4^Act.PU/p38b^{EPSV.UAS.Tag:GST}

(Seisenbacher et al., 2011)

Not rescued by

p38b^d27 is not rescued by Scer\GAL4^Act.PU/p38b^{AGF.UAS.Tag:GST}

(Seisenbacher et al., 2011)

p38b^d27 is not rescued by Scer\GAL4^Act.PU/p38b^{KR.UAS.Tag:GST}

(Seisenbacher et al., 2011)

Comments

Expression of p38b^{Scer\UAS.T:Sjap\GST} under the control of Scer\GAL4^Act.PU rescues the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl.

Expression of p38b^{AGF.Scer\UAS.T:Sjap\GST} under the control of Scer\GAL4^Act.PU fails to rescue the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with 0.1M NaCl. The larvae die when raised on 0.2M NaCl.

Expression of p38b^{KR.Scer\UAS.T:Sjap\GST} under the control of Scer\GAL4^Act.PU fails to rescue the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with 0.1M NaCl. The larvae die when raised on 0.2M NaCl.

Expression of p38b^{Scer\UAS.EPSV.T:Sjap\GST} under the control of Scer\GAL4^Act.PU partially rescues the "black dot" phenotype seen in the hindgut of p38b^d27 mutant larvae on normal food and food supplemented with either 0.1M or 0.2M NaCl.

(Seisenbacher et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

p38b^d27

(Santabárbara-Ruiz et al., 2015, Seisenbacher et al., 2011, Cully et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (3)

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